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Apparent histological changes of adipocytes after treatment with CL 316,243, a β-3-adrenergic receptor agonist.

Ghorbani M, Teimourian S, Farzad R, Asl NN - Drug Des Devel Ther (2015)

Bottom Line: There was no effect of CL on food intake.Detection of uncoupling protein 1 (UCP1) in RWAT was confirmed qualitatively by both immunohistochemistry and immunofluorescence using a rabbit anti rat UCP1 antibody which showed the appearance of a marked increase of this protein in the adipose tissue.Stained semi-thin sections (0.5 μm) also demonstrated abundant nuclei in multilocular adipocytes, in endothelial cells associated with the vasculature, and in interstitial cells.

View Article: PubMed Central - PubMed

Affiliation: Research and Development Department, Pasteur Institute of Iran, Tehran, Iran ; Department of Biochemistry, University of Ottawa, Ottawa, ON, Canada.

ABSTRACT

Background and objectives: The objective of this experiment was to study the effect of CL 316,243 (CL) (a highly selective β3-adrenergic receptor agonist) on cellular changes occurring in retroperitoneal white adipose tissue (RWAT) of lean and obese rats.

Methods: Ten-month-old lean and obese Zucker rats were implanted subcutaneously with osmotic mini-pumps, infusing either saline or CL (1 mg/kg body weight/day) for 4 weeks.

Results: There was no effect of CL on food intake. However, the resting metabolic rate in lean and obese rats increased by 55% and 96% per rat, respectively. Total RWAT weight decreased in both lean and obese rats under influence of CL treatment by 65% and 38%, respectively. Total body weight and body fat were lower in CL treated rats. Detection of uncoupling protein 1 (UCP1) in RWAT was confirmed qualitatively by both immunohistochemistry and immunofluorescence using a rabbit anti rat UCP1 antibody which showed the appearance of a marked increase of this protein in the adipose tissue. Stained semi-thin sections (0.5 μm) also demonstrated abundant nuclei in multilocular adipocytes, in endothelial cells associated with the vasculature, and in interstitial cells. In CL-treated obese rats, a clustering of several multilocular cells around the periphery of a white adipocyte was seen.

Conclusion: These results indicate that treatment of both lean and obese Zucker rats with CL induces extensive remodeling of RWAT that includes shrinkage of white adipose tissue, appearance of abundant multilocular cells in RWAT together with the appearance of a marked increase of UCP, preferentially in lean rats.

No MeSH data available.


Related in: MedlinePlus

Serum fatty acids level in lean and obese rats treated with CL 316,243.Notes: Bars are mean ± SEM for ten rats. *Significant values are based on P<0.05.Abbreviations: CL, CL 316,243; SEM, standard error of the mean; FAs, fatty acids.
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f11-dddt-9-669: Serum fatty acids level in lean and obese rats treated with CL 316,243.Notes: Bars are mean ± SEM for ten rats. *Significant values are based on P<0.05.Abbreviations: CL, CL 316,243; SEM, standard error of the mean; FAs, fatty acids.

Mentions: Treatment with CL also decreased the serum insulin level in fa/fa rats. In oral glucose tolerance test, serum insulin and blood glucose levels were significantly higher in the obese rats treated with saline after feeding with 2 mg glucose/g body weight as compared with lean rats treated with saline. Treatment with CL significantly decreased both serum insulin and blood glucose levels in fa/fa rats (Figure 10A and B). Measurement of serum FAs also showed a marked decrease in obese rats treated with CL which was accompanied by normoglycemia and improvement of insulin resistance indicating that β3-adrenergic responsiveness is present in these rats (Figure 11).


Apparent histological changes of adipocytes after treatment with CL 316,243, a β-3-adrenergic receptor agonist.

Ghorbani M, Teimourian S, Farzad R, Asl NN - Drug Des Devel Ther (2015)

Serum fatty acids level in lean and obese rats treated with CL 316,243.Notes: Bars are mean ± SEM for ten rats. *Significant values are based on P<0.05.Abbreviations: CL, CL 316,243; SEM, standard error of the mean; FAs, fatty acids.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4334338&req=5

f11-dddt-9-669: Serum fatty acids level in lean and obese rats treated with CL 316,243.Notes: Bars are mean ± SEM for ten rats. *Significant values are based on P<0.05.Abbreviations: CL, CL 316,243; SEM, standard error of the mean; FAs, fatty acids.
Mentions: Treatment with CL also decreased the serum insulin level in fa/fa rats. In oral glucose tolerance test, serum insulin and blood glucose levels were significantly higher in the obese rats treated with saline after feeding with 2 mg glucose/g body weight as compared with lean rats treated with saline. Treatment with CL significantly decreased both serum insulin and blood glucose levels in fa/fa rats (Figure 10A and B). Measurement of serum FAs also showed a marked decrease in obese rats treated with CL which was accompanied by normoglycemia and improvement of insulin resistance indicating that β3-adrenergic responsiveness is present in these rats (Figure 11).

Bottom Line: There was no effect of CL on food intake.Detection of uncoupling protein 1 (UCP1) in RWAT was confirmed qualitatively by both immunohistochemistry and immunofluorescence using a rabbit anti rat UCP1 antibody which showed the appearance of a marked increase of this protein in the adipose tissue.Stained semi-thin sections (0.5 μm) also demonstrated abundant nuclei in multilocular adipocytes, in endothelial cells associated with the vasculature, and in interstitial cells.

View Article: PubMed Central - PubMed

Affiliation: Research and Development Department, Pasteur Institute of Iran, Tehran, Iran ; Department of Biochemistry, University of Ottawa, Ottawa, ON, Canada.

ABSTRACT

Background and objectives: The objective of this experiment was to study the effect of CL 316,243 (CL) (a highly selective β3-adrenergic receptor agonist) on cellular changes occurring in retroperitoneal white adipose tissue (RWAT) of lean and obese rats.

Methods: Ten-month-old lean and obese Zucker rats were implanted subcutaneously with osmotic mini-pumps, infusing either saline or CL (1 mg/kg body weight/day) for 4 weeks.

Results: There was no effect of CL on food intake. However, the resting metabolic rate in lean and obese rats increased by 55% and 96% per rat, respectively. Total RWAT weight decreased in both lean and obese rats under influence of CL treatment by 65% and 38%, respectively. Total body weight and body fat were lower in CL treated rats. Detection of uncoupling protein 1 (UCP1) in RWAT was confirmed qualitatively by both immunohistochemistry and immunofluorescence using a rabbit anti rat UCP1 antibody which showed the appearance of a marked increase of this protein in the adipose tissue. Stained semi-thin sections (0.5 μm) also demonstrated abundant nuclei in multilocular adipocytes, in endothelial cells associated with the vasculature, and in interstitial cells. In CL-treated obese rats, a clustering of several multilocular cells around the periphery of a white adipocyte was seen.

Conclusion: These results indicate that treatment of both lean and obese Zucker rats with CL induces extensive remodeling of RWAT that includes shrinkage of white adipose tissue, appearance of abundant multilocular cells in RWAT together with the appearance of a marked increase of UCP, preferentially in lean rats.

No MeSH data available.


Related in: MedlinePlus