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Detection of anthocyanins/anthocyanidins in animal tissues.

Aqil F, Vadhanam MV, Jeyabalan J, Cai J, Singh IP, Gupta RC - J. Agric. Food Chem. (2014)

Bottom Line: All five anthocyanidins present in the blueberry were detected in the lung tissue using improved methods.The effect of various solvents on the stability of anthocyanins and their recovery from the biomatrix was analyzed.Although anthocyanins were not detected, cyanidin was detected by UPLC-PDA and other anthocyanidins were detected by LC-MS, following conversion to anthocyanidins and selective extraction in isoamyl alcohol.

View Article: PubMed Central - PubMed

Affiliation: James Graham Brown Cancer Center, ‡Department of Medicine, and #Department of Pharmacology and Toxicology, University of Louisville , Louisville, Kentucky 40202, United States.

ABSTRACT
Dietary polyphenols may contribute to the prevention of several degenerative diseases, including cancer. Anthocyanins have been shown to possess potential anticancer activity. The aim of this study was to determine anthocyanin bioavailability in lung tissue of mice fed a blueberry diet (5% w/w) for 10 days or a bolus dose (10 mg/mouse; po) of a native mixture of bilberry anthocyanidins. All five anthocyanidins present in the blueberry were detected in the lung tissue using improved methods. The effect of various solvents on the stability of anthocyanins and their recovery from the biomatrix was analyzed. Detection of anthocyanins and their metabolites was performed by UPLC and LC-MS. Although anthocyanins were not detected, cyanidin was detected by UPLC-PDA and other anthocyanidins were detected by LC-MS, following conversion to anthocyanidins and selective extraction in isoamyl alcohol. The results show that anthocyanins can be detected in lung tissue of blueberry-fed mice and thus are bioavailable beyond the gastrointestinal tract.

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Related in: MedlinePlus

Blueberry fruits andchemical structure of anthocyanins and anthocyanidinsfound in blueberry/bilberry. Shaded portion shows the difference inanthocyanidins and anthocyanins.
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fig1: Blueberry fruits andchemical structure of anthocyanins and anthocyanidinsfound in blueberry/bilberry. Shaded portion shows the difference inanthocyanidins and anthocyanins.

Mentions: Blueberryextract was applied onto the XAD-761/HP-20 column (1:1)for the enrichment of anthocyanins and other polyphenols, and theenriched extract was hydrolyzed to convert the glycones to aglycones(i.e., anthocyanins to anthocyanidins; Figure 1). The enriched anthocyanidins were extracted in isoamyl alcoholand further purified by C18 column chromatography. The final extractcontained highly pure (>94%) anthocyanidins (Figure 2). When analyzed by UPLC, the purified extract showed fiveanthocyanidins in the following descending order: Dp (33%), Cy (28%),Pt (16%), Ma (16%), and Pe (7%); a small amount of quercetin was alsofound in the sample (Figure 2).


Detection of anthocyanins/anthocyanidins in animal tissues.

Aqil F, Vadhanam MV, Jeyabalan J, Cai J, Singh IP, Gupta RC - J. Agric. Food Chem. (2014)

Blueberry fruits andchemical structure of anthocyanins and anthocyanidinsfound in blueberry/bilberry. Shaded portion shows the difference inanthocyanidins and anthocyanins.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4334289&req=5

fig1: Blueberry fruits andchemical structure of anthocyanins and anthocyanidinsfound in blueberry/bilberry. Shaded portion shows the difference inanthocyanidins and anthocyanins.
Mentions: Blueberryextract was applied onto the XAD-761/HP-20 column (1:1)for the enrichment of anthocyanins and other polyphenols, and theenriched extract was hydrolyzed to convert the glycones to aglycones(i.e., anthocyanins to anthocyanidins; Figure 1). The enriched anthocyanidins were extracted in isoamyl alcoholand further purified by C18 column chromatography. The final extractcontained highly pure (>94%) anthocyanidins (Figure 2). When analyzed by UPLC, the purified extract showed fiveanthocyanidins in the following descending order: Dp (33%), Cy (28%),Pt (16%), Ma (16%), and Pe (7%); a small amount of quercetin was alsofound in the sample (Figure 2).

Bottom Line: All five anthocyanidins present in the blueberry were detected in the lung tissue using improved methods.The effect of various solvents on the stability of anthocyanins and their recovery from the biomatrix was analyzed.Although anthocyanins were not detected, cyanidin was detected by UPLC-PDA and other anthocyanidins were detected by LC-MS, following conversion to anthocyanidins and selective extraction in isoamyl alcohol.

View Article: PubMed Central - PubMed

Affiliation: James Graham Brown Cancer Center, ‡Department of Medicine, and #Department of Pharmacology and Toxicology, University of Louisville , Louisville, Kentucky 40202, United States.

ABSTRACT
Dietary polyphenols may contribute to the prevention of several degenerative diseases, including cancer. Anthocyanins have been shown to possess potential anticancer activity. The aim of this study was to determine anthocyanin bioavailability in lung tissue of mice fed a blueberry diet (5% w/w) for 10 days or a bolus dose (10 mg/mouse; po) of a native mixture of bilberry anthocyanidins. All five anthocyanidins present in the blueberry were detected in the lung tissue using improved methods. The effect of various solvents on the stability of anthocyanins and their recovery from the biomatrix was analyzed. Detection of anthocyanins and their metabolites was performed by UPLC and LC-MS. Although anthocyanins were not detected, cyanidin was detected by UPLC-PDA and other anthocyanidins were detected by LC-MS, following conversion to anthocyanidins and selective extraction in isoamyl alcohol. The results show that anthocyanins can be detected in lung tissue of blueberry-fed mice and thus are bioavailable beyond the gastrointestinal tract.

Show MeSH
Related in: MedlinePlus