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Chemopreventive and therapeutic activity of dietary blueberry against estrogen-mediated breast cancer.

Jeyabalan J, Aqil F, Munagala R, Annamalai L, Vadhanam MV, Gupta RC - J. Agric. Food Chem. (2014)

Bottom Line: Berries are gaining increasing importance lately for their chemopreventive and therapeutic potential against several cancers.The effect on mammary tumorigenesis was largely due to down-regulation of CYP 1A1 and ER-α gene expression and also favorable modulation of microRNA (miR-18a and miR-34c) levels.These data suggest that the blueberry blend tested is effective in inhibiting E2-mediated mammary tumorigenesis in both preventive and therapeutic modes.

View Article: PubMed Central - PubMed

Affiliation: James Graham Brown Cancer Center, ‡Department of Medicine, and #Department of Pharmacology and Toxicology, University of Louisville , Louisville, Kentucky 40202, United States.

ABSTRACT
Berries are gaining increasing importance lately for their chemopreventive and therapeutic potential against several cancers. In earlier studies, a blueberry-supplemented diet has shown protection against 17β-estradiol (E2)-mediated mammary tumorigenesis. This study tested both preventive and therapeutic activities of diet supplemented with whole blueberry powder (50:50 blend of Tifblue and Rubel). Animals received 5% blueberry diet, either 2 weeks prior to or 12 weeks after E2 treatment in preventive and therapeutic groups, respectively. Both interventions delayed the tumor latency for palpable mammary tumors by 28 and 37 days, respectively. Tumor volume and multiplicity were also reduced significantly in both modes. The effect on mammary tumorigenesis was largely due to down-regulation of CYP 1A1 and ER-α gene expression and also favorable modulation of microRNA (miR-18a and miR-34c) levels. These data suggest that the blueberry blend tested is effective in inhibiting E2-mediated mammary tumorigenesis in both preventive and therapeutic modes.

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Related in: MedlinePlus

Proliferative index and effect of blueberry diet with or withoutestrogen (E2) treatment. (A) Immunohistochemical stainingfor proliferating cell nuclear antigen (PCNA). Representative photomicrographsare 20× magnification of normal and hyperplastic mammary tissue.(B) Graph denotes the percentage of deeply stained cells for PCNAin mammary tissues (n = 5). a Statisticallysignificant from untreated control (p = 0.0095). b Statistically significant from E2-treatedcontrol (p = 0.0373 and p = 0.0251).
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fig4: Proliferative index and effect of blueberry diet with or withoutestrogen (E2) treatment. (A) Immunohistochemical stainingfor proliferating cell nuclear antigen (PCNA). Representative photomicrographsare 20× magnification of normal and hyperplastic mammary tissue.(B) Graph denotes the percentage of deeply stained cells for PCNAin mammary tissues (n = 5). a Statisticallysignificant from untreated control (p = 0.0095). b Statistically significant from E2-treatedcontrol (p = 0.0373 and p = 0.0251).

Mentions: Cell proliferation was analyzedby staining tumor and adjacent mammary tissue for PCNA protein markersusing antibody-based assay. After 25 weeks, there was no significantdifference in cell proliferation among animals receiving either controlor 2.5 and 5% BB diets. However, the E2 treatment increasedthe cell proliferation to nearly 6-fold (p = 0.0095)(Figure 4). This increase in the cell proliferationwas significantly offset by 34 and 35% with 5% BB diet administeredin chemopreventive and therapeutic mode, respectively (Figure 4); the effect was insignificant with the 2.5% BBdiet.


Chemopreventive and therapeutic activity of dietary blueberry against estrogen-mediated breast cancer.

Jeyabalan J, Aqil F, Munagala R, Annamalai L, Vadhanam MV, Gupta RC - J. Agric. Food Chem. (2014)

Proliferative index and effect of blueberry diet with or withoutestrogen (E2) treatment. (A) Immunohistochemical stainingfor proliferating cell nuclear antigen (PCNA). Representative photomicrographsare 20× magnification of normal and hyperplastic mammary tissue.(B) Graph denotes the percentage of deeply stained cells for PCNAin mammary tissues (n = 5). a Statisticallysignificant from untreated control (p = 0.0095). b Statistically significant from E2-treatedcontrol (p = 0.0373 and p = 0.0251).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4334276&req=5

fig4: Proliferative index and effect of blueberry diet with or withoutestrogen (E2) treatment. (A) Immunohistochemical stainingfor proliferating cell nuclear antigen (PCNA). Representative photomicrographsare 20× magnification of normal and hyperplastic mammary tissue.(B) Graph denotes the percentage of deeply stained cells for PCNAin mammary tissues (n = 5). a Statisticallysignificant from untreated control (p = 0.0095). b Statistically significant from E2-treatedcontrol (p = 0.0373 and p = 0.0251).
Mentions: Cell proliferation was analyzedby staining tumor and adjacent mammary tissue for PCNA protein markersusing antibody-based assay. After 25 weeks, there was no significantdifference in cell proliferation among animals receiving either controlor 2.5 and 5% BB diets. However, the E2 treatment increasedthe cell proliferation to nearly 6-fold (p = 0.0095)(Figure 4). This increase in the cell proliferationwas significantly offset by 34 and 35% with 5% BB diet administeredin chemopreventive and therapeutic mode, respectively (Figure 4); the effect was insignificant with the 2.5% BBdiet.

Bottom Line: Berries are gaining increasing importance lately for their chemopreventive and therapeutic potential against several cancers.The effect on mammary tumorigenesis was largely due to down-regulation of CYP 1A1 and ER-α gene expression and also favorable modulation of microRNA (miR-18a and miR-34c) levels.These data suggest that the blueberry blend tested is effective in inhibiting E2-mediated mammary tumorigenesis in both preventive and therapeutic modes.

View Article: PubMed Central - PubMed

Affiliation: James Graham Brown Cancer Center, ‡Department of Medicine, and #Department of Pharmacology and Toxicology, University of Louisville , Louisville, Kentucky 40202, United States.

ABSTRACT
Berries are gaining increasing importance lately for their chemopreventive and therapeutic potential against several cancers. In earlier studies, a blueberry-supplemented diet has shown protection against 17β-estradiol (E2)-mediated mammary tumorigenesis. This study tested both preventive and therapeutic activities of diet supplemented with whole blueberry powder (50:50 blend of Tifblue and Rubel). Animals received 5% blueberry diet, either 2 weeks prior to or 12 weeks after E2 treatment in preventive and therapeutic groups, respectively. Both interventions delayed the tumor latency for palpable mammary tumors by 28 and 37 days, respectively. Tumor volume and multiplicity were also reduced significantly in both modes. The effect on mammary tumorigenesis was largely due to down-regulation of CYP 1A1 and ER-α gene expression and also favorable modulation of microRNA (miR-18a and miR-34c) levels. These data suggest that the blueberry blend tested is effective in inhibiting E2-mediated mammary tumorigenesis in both preventive and therapeutic modes.

Show MeSH
Related in: MedlinePlus