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Assesment of endocrinal and biochemical entities through liquid chromatography-tandem mass spectrometry/mass spectrometer: Inter-relative investigation of the interaction based cardiovascular formulation.

Das R, Pal TK - J Pharm Bioallied Sci (2015 Jan-Mar)

Bottom Line: The selectivity, specificity, linearity, precision, accuracy, extraction recovery, limit of detection and limit of quantification, stability were the essential points of validation of the developed methodology.And the significance of each endogenous analyte data were based on P ≥ 0.001.This concludes that the cardiovascular dosage formulation entrenched in the market are not synergistic and effective compared with a single drug as antihypertensive drug.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutical Technology, Bioequivalence Study Center, Jadavpur University, Kolkata, West Bengal, India.

ABSTRACT

Background: Combinatory oral dosage treatment of atorvastatin (ATVS) and olmesartan (OLM) drugs to cardiovascular patients reflects unpredicted results instead of its individual therapy, which was accessed on quantification of endocrinal and biochemicals of plasma through liquid chromatography-tandem mass spectrometry/mass spectrometer (LCMS/MS).

Objective: Mission was to track the remarkable biochemical variation in the plasma after induction of the combined formulation, to evaluate the pharma-market rumor on its efficiency.

Methods: To fulfil undergoing research objectives for digging-up of market insult, human patient volunteers were chosen according to the required criteria along with bioethical regulation. A sensitive, rapid and precise method was developed and validated to estimate aldosterone (ALD), angiotensin (ANG-II) and the Mevalonate (MVA) not Mevalonic acid through LCMS/MS over least samples of cardiovascular patients. Level of each endogenous biochemicals were determined in three stages - without drugs, with a single drug (OLM/ATVS) and with their combination that was then correlate with blood pressure of respective volunteers.

Result and discussion: Comparative and correlative studies panaroma among these analytes was detected. The selectivity, specificity, linearity, precision, accuracy, extraction recovery, limit of detection and limit of quantification, stability were the essential points of validation of the developed methodology. And the significance of each endogenous analyte data were based on P ≥ 0.001. Thus, low value of ALD and reciprocally higher in ANG-II on administered single drug than its combination and equal concentration of mevalonate in both stages, was discovered.

Conclusion: This concludes that the cardiovascular dosage formulation entrenched in the market are not synergistic and effective compared with a single drug as antihypertensive drug.

No MeSH data available.


Comparative chromatograms trace of standard and internal standard for concentration of aldosterone, angiotensin II and MVA at 50, 5, 50 ng/ml respectively under plotted recovered area versus retention time (minutes) with respect to multiple reaction monitoring scan
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Figure 1: Comparative chromatograms trace of standard and internal standard for concentration of aldosterone, angiotensin II and MVA at 50, 5, 50 ng/ml respectively under plotted recovered area versus retention time (minutes) with respect to multiple reaction monitoring scan

Mentions: A constitutional studies of data among all biochemical analysis behalf of linearity, accuracy and precision, extraction recovery, stability and sensitivity exhibits a best validator for endogenous biochemical quantity as well as qualities. The recovered area and retention time for specific concentration ranges was calibrated with standard versus internal standard chromatograms [Figure 1] to validated and trace/scan the chromatograms of plasma biochemical analytes of 12 patients.


Assesment of endocrinal and biochemical entities through liquid chromatography-tandem mass spectrometry/mass spectrometer: Inter-relative investigation of the interaction based cardiovascular formulation.

Das R, Pal TK - J Pharm Bioallied Sci (2015 Jan-Mar)

Comparative chromatograms trace of standard and internal standard for concentration of aldosterone, angiotensin II and MVA at 50, 5, 50 ng/ml respectively under plotted recovered area versus retention time (minutes) with respect to multiple reaction monitoring scan
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4333628&req=5

Figure 1: Comparative chromatograms trace of standard and internal standard for concentration of aldosterone, angiotensin II and MVA at 50, 5, 50 ng/ml respectively under plotted recovered area versus retention time (minutes) with respect to multiple reaction monitoring scan
Mentions: A constitutional studies of data among all biochemical analysis behalf of linearity, accuracy and precision, extraction recovery, stability and sensitivity exhibits a best validator for endogenous biochemical quantity as well as qualities. The recovered area and retention time for specific concentration ranges was calibrated with standard versus internal standard chromatograms [Figure 1] to validated and trace/scan the chromatograms of plasma biochemical analytes of 12 patients.

Bottom Line: The selectivity, specificity, linearity, precision, accuracy, extraction recovery, limit of detection and limit of quantification, stability were the essential points of validation of the developed methodology.And the significance of each endogenous analyte data were based on P ≥ 0.001.This concludes that the cardiovascular dosage formulation entrenched in the market are not synergistic and effective compared with a single drug as antihypertensive drug.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutical Technology, Bioequivalence Study Center, Jadavpur University, Kolkata, West Bengal, India.

ABSTRACT

Background: Combinatory oral dosage treatment of atorvastatin (ATVS) and olmesartan (OLM) drugs to cardiovascular patients reflects unpredicted results instead of its individual therapy, which was accessed on quantification of endocrinal and biochemicals of plasma through liquid chromatography-tandem mass spectrometry/mass spectrometer (LCMS/MS).

Objective: Mission was to track the remarkable biochemical variation in the plasma after induction of the combined formulation, to evaluate the pharma-market rumor on its efficiency.

Methods: To fulfil undergoing research objectives for digging-up of market insult, human patient volunteers were chosen according to the required criteria along with bioethical regulation. A sensitive, rapid and precise method was developed and validated to estimate aldosterone (ALD), angiotensin (ANG-II) and the Mevalonate (MVA) not Mevalonic acid through LCMS/MS over least samples of cardiovascular patients. Level of each endogenous biochemicals were determined in three stages - without drugs, with a single drug (OLM/ATVS) and with their combination that was then correlate with blood pressure of respective volunteers.

Result and discussion: Comparative and correlative studies panaroma among these analytes was detected. The selectivity, specificity, linearity, precision, accuracy, extraction recovery, limit of detection and limit of quantification, stability were the essential points of validation of the developed methodology. And the significance of each endogenous analyte data were based on P ≥ 0.001. Thus, low value of ALD and reciprocally higher in ANG-II on administered single drug than its combination and equal concentration of mevalonate in both stages, was discovered.

Conclusion: This concludes that the cardiovascular dosage formulation entrenched in the market are not synergistic and effective compared with a single drug as antihypertensive drug.

No MeSH data available.