Limits...
Anti-Candida activity of Quercus infectoria gall extracts against Candida species.

Baharuddin NS, Abdullah H, Abdul Wahab WN - J Pharm Bioallied Sci (2015 Jan-Mar)

Bottom Line: Potential phytochemical group in the crude extracts was screened by phytochemical qualitative tests and subsequently subjected to the gas chromatography-mass spectrometry analysis.Both methanol and aqueous extracts displayed substantial anti-Candida activity and pyrogallol was the major component of both crude extracts.Data from current study suggested that Q. infectoria gall extracts are a potential source to be developed as anti-candidiasis.

View Article: PubMed Central - PubMed

Affiliation: Biomedicine Programme, School of Health Sciences, Universiti Sains Malaysia, Kubang Kerian 16150, Kelantan.

ABSTRACT

Background: Galls of Quercus infectoria have been traditionally used to treat common ailments, including yeast infections caused by Candida species.

Objective: This study aimed to evaluate the in vitro anti-Candida activity of Q. infectoria gall extracts against selected Candida species.

Materials and methods: Methanol and aqueous extracts of Q. infectoria galls were tested for anti-Candida activity against Candida albicans, Candida krusei, Candida glabrata, Candida parapsilosis and Candida tropicalis. The minimum inhibitory concentrations were determined using the two-fold serial dilution technique of concentrations ranging from 16 mg/ml to 0.03 mg/ml. After 24 h, the minimum fungicidal concentrations were determined by subculturing the wells, which showed no turbidity on the agar plate. Potential phytochemical group in the crude extracts was screened by phytochemical qualitative tests and subsequently subjected to the gas chromatography-mass spectrometry analysis.

Results: Both methanol and aqueous extracts displayed substantial anti-Candida activity and pyrogallol was the major component of both crude extracts.

Conclusions: Data from current study suggested that Q. infectoria gall extracts are a potential source to be developed as anti-candidiasis.

No MeSH data available.


Related in: MedlinePlus

Chromatogram of methanol extract (a) and aquoeus extract (b) of Quercus infectoria gall by gas chromatography-mass spectrometry (GC-MS) Analysis. The GC-MS spectrum at retention time 14.50 min of major compound of the 1,2,3-Benzenetriol (pyrogallol) with molecular formula of C6H6O3 and molecular weight of 126.0 g/mol (c)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4333621&req=5

Figure 2: Chromatogram of methanol extract (a) and aquoeus extract (b) of Quercus infectoria gall by gas chromatography-mass spectrometry (GC-MS) Analysis. The GC-MS spectrum at retention time 14.50 min of major compound of the 1,2,3-Benzenetriol (pyrogallol) with molecular formula of C6H6O3 and molecular weight of 126.0 g/mol (c)

Mentions: Preliminary phytochemical screening was performed to establish the profile of gall extracts for its chemical composition. Both methanol and aqueous Q. infectoria gall extracts showed the presence of tannins [Table 3]. The result of GC-mass spectrometry (GC-MS) analysis of the crude extracts of Q. infectoria revealed two major compounds scanned by the NISTO5a.L database. The active principle, retention time (RT), molecular weight and molecular structure were presented in Figure 2. 1, 2, 3 - benzenetriol (pyrogallol) in both methanol and aqueous extracts of Q. infectoria gall was identified in high percentage, which accounted for 81.66% and 100% of the total respectively.


Anti-Candida activity of Quercus infectoria gall extracts against Candida species.

Baharuddin NS, Abdullah H, Abdul Wahab WN - J Pharm Bioallied Sci (2015 Jan-Mar)

Chromatogram of methanol extract (a) and aquoeus extract (b) of Quercus infectoria gall by gas chromatography-mass spectrometry (GC-MS) Analysis. The GC-MS spectrum at retention time 14.50 min of major compound of the 1,2,3-Benzenetriol (pyrogallol) with molecular formula of C6H6O3 and molecular weight of 126.0 g/mol (c)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4333621&req=5

Figure 2: Chromatogram of methanol extract (a) and aquoeus extract (b) of Quercus infectoria gall by gas chromatography-mass spectrometry (GC-MS) Analysis. The GC-MS spectrum at retention time 14.50 min of major compound of the 1,2,3-Benzenetriol (pyrogallol) with molecular formula of C6H6O3 and molecular weight of 126.0 g/mol (c)
Mentions: Preliminary phytochemical screening was performed to establish the profile of gall extracts for its chemical composition. Both methanol and aqueous Q. infectoria gall extracts showed the presence of tannins [Table 3]. The result of GC-mass spectrometry (GC-MS) analysis of the crude extracts of Q. infectoria revealed two major compounds scanned by the NISTO5a.L database. The active principle, retention time (RT), molecular weight and molecular structure were presented in Figure 2. 1, 2, 3 - benzenetriol (pyrogallol) in both methanol and aqueous extracts of Q. infectoria gall was identified in high percentage, which accounted for 81.66% and 100% of the total respectively.

Bottom Line: Potential phytochemical group in the crude extracts was screened by phytochemical qualitative tests and subsequently subjected to the gas chromatography-mass spectrometry analysis.Both methanol and aqueous extracts displayed substantial anti-Candida activity and pyrogallol was the major component of both crude extracts.Data from current study suggested that Q. infectoria gall extracts are a potential source to be developed as anti-candidiasis.

View Article: PubMed Central - PubMed

Affiliation: Biomedicine Programme, School of Health Sciences, Universiti Sains Malaysia, Kubang Kerian 16150, Kelantan.

ABSTRACT

Background: Galls of Quercus infectoria have been traditionally used to treat common ailments, including yeast infections caused by Candida species.

Objective: This study aimed to evaluate the in vitro anti-Candida activity of Q. infectoria gall extracts against selected Candida species.

Materials and methods: Methanol and aqueous extracts of Q. infectoria galls were tested for anti-Candida activity against Candida albicans, Candida krusei, Candida glabrata, Candida parapsilosis and Candida tropicalis. The minimum inhibitory concentrations were determined using the two-fold serial dilution technique of concentrations ranging from 16 mg/ml to 0.03 mg/ml. After 24 h, the minimum fungicidal concentrations were determined by subculturing the wells, which showed no turbidity on the agar plate. Potential phytochemical group in the crude extracts was screened by phytochemical qualitative tests and subsequently subjected to the gas chromatography-mass spectrometry analysis.

Results: Both methanol and aqueous extracts displayed substantial anti-Candida activity and pyrogallol was the major component of both crude extracts.

Conclusions: Data from current study suggested that Q. infectoria gall extracts are a potential source to be developed as anti-candidiasis.

No MeSH data available.


Related in: MedlinePlus