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Maternal Deprivation of Lewis Rat Pups Increases the Severity of Experi-mental Periodontitis in Adulthood.

Breivik T, Gundersen Y, Murison R, Turner JD, Muller CP, Gjermo P, Opstad K - Open Dent J (2015)

Bottom Line: Early life adverse events may influence susceptibility/resistance to chronic inflammatory diseases later in life by permanently dysregulating brain-controlled immune-regulatory systems.LPS provoked a significantly lower increase in circulating levels of the cytokine TGF-1β in MD and HD rats, but there were no significant differences in levels of the stress hormone corticosterone.Stressful environmental exposures in very early life may alter immune responses in a manner that influences susceptibility/resistance to periodontitis.

View Article: PubMed Central - PubMed

Affiliation: Department of Periodontology, Faculty of Dentistry, University of Oslo, Norway ; Norwegian Defence Research Establishment, Division for Protection, Kjeller, Norway.

ABSTRACT

Background and objective: Early life adverse events may influence susceptibility/resistance to chronic inflammatory diseases later in life by permanently dysregulating brain-controlled immune-regulatory systems. We have investigated the impact of infant-mother separation during early postnatal life on the severity of experimental periodontitis, as well as systemic stress and immune responses, in adulthood.

Material and methods: Pups of periodontitis resistant Lewis rats were separated from their mothers for 3 h daily during postnatal days 2-14 (termed maternal deprivation; MD), separated for 15 min daily during the same time period (termed handling; HD), or left undisturbed. As adults, their behaviour was tested in a novel stressful situation, and ligature-induced periodontitis applied for 21 days. Two h before sacrifice all rats were exposed to a gram-negative bacterial lipopolysaccharide (LPS) challenge to induce a robust immune and stress response.

Results: Compared to undisturbed controls, MD rats developed significantly more periodontal bone loss as adults, whereas HD rats showed a tendency to less disease. MD and HD rats exhibited depression-like behaviour in a novel open field test, while MD rats showed higher glucocorticoid receptor (Gr) expression in the hippocampus, and HD rats had altered methylation of genes involved in the expression of hippocampal Gr. LPS provoked a significantly lower increase in circulating levels of the cytokine TGF-1β in MD and HD rats, but there were no significant differences in levels of the stress hormone corticosterone.

Conclusion: Stressful environmental exposures in very early life may alter immune responses in a manner that influences susceptibility/resistance to periodontitis.

No MeSH data available.


Related in: MedlinePlus

Levels of transforming growth factor (TGF)-1beta in serum 2 h after intraperitoneal injection of LPS (150 microg/kg) in male andfemale control rats, handled (HD) rats, and maternal deprived (MD) rats. p < 0.05 between controls and HD rats as well as MD rats in bothmales and females.
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Figure 2: Levels of transforming growth factor (TGF)-1beta in serum 2 h after intraperitoneal injection of LPS (150 microg/kg) in male andfemale control rats, handled (HD) rats, and maternal deprived (MD) rats. p < 0.05 between controls and HD rats as well as MD rats in bothmales and females.

Mentions: Overall, serum TGF-1β levels were higher in males than in females rats, p < 0.05. The MD rats had significantly lower serum TGF-1β levels 2 h after LPS stimulation compared with controls in both males and females (Fig. 2). In MD male rats the serum TGF-1β levels were 56.3 ± 3.3 pg/ml versus 63.2 ± 7.0 pg/ml in male controls (p < 0.05), while the values for female rats were 51.0 ± 5.2 pg/ml versus 57.9 ± 7.1 pg/ml (p < 0.05).


Maternal Deprivation of Lewis Rat Pups Increases the Severity of Experi-mental Periodontitis in Adulthood.

Breivik T, Gundersen Y, Murison R, Turner JD, Muller CP, Gjermo P, Opstad K - Open Dent J (2015)

Levels of transforming growth factor (TGF)-1beta in serum 2 h after intraperitoneal injection of LPS (150 microg/kg) in male andfemale control rats, handled (HD) rats, and maternal deprived (MD) rats. p < 0.05 between controls and HD rats as well as MD rats in bothmales and females.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4333617&req=5

Figure 2: Levels of transforming growth factor (TGF)-1beta in serum 2 h after intraperitoneal injection of LPS (150 microg/kg) in male andfemale control rats, handled (HD) rats, and maternal deprived (MD) rats. p < 0.05 between controls and HD rats as well as MD rats in bothmales and females.
Mentions: Overall, serum TGF-1β levels were higher in males than in females rats, p < 0.05. The MD rats had significantly lower serum TGF-1β levels 2 h after LPS stimulation compared with controls in both males and females (Fig. 2). In MD male rats the serum TGF-1β levels were 56.3 ± 3.3 pg/ml versus 63.2 ± 7.0 pg/ml in male controls (p < 0.05), while the values for female rats were 51.0 ± 5.2 pg/ml versus 57.9 ± 7.1 pg/ml (p < 0.05).

Bottom Line: Early life adverse events may influence susceptibility/resistance to chronic inflammatory diseases later in life by permanently dysregulating brain-controlled immune-regulatory systems.LPS provoked a significantly lower increase in circulating levels of the cytokine TGF-1β in MD and HD rats, but there were no significant differences in levels of the stress hormone corticosterone.Stressful environmental exposures in very early life may alter immune responses in a manner that influences susceptibility/resistance to periodontitis.

View Article: PubMed Central - PubMed

Affiliation: Department of Periodontology, Faculty of Dentistry, University of Oslo, Norway ; Norwegian Defence Research Establishment, Division for Protection, Kjeller, Norway.

ABSTRACT

Background and objective: Early life adverse events may influence susceptibility/resistance to chronic inflammatory diseases later in life by permanently dysregulating brain-controlled immune-regulatory systems. We have investigated the impact of infant-mother separation during early postnatal life on the severity of experimental periodontitis, as well as systemic stress and immune responses, in adulthood.

Material and methods: Pups of periodontitis resistant Lewis rats were separated from their mothers for 3 h daily during postnatal days 2-14 (termed maternal deprivation; MD), separated for 15 min daily during the same time period (termed handling; HD), or left undisturbed. As adults, their behaviour was tested in a novel stressful situation, and ligature-induced periodontitis applied for 21 days. Two h before sacrifice all rats were exposed to a gram-negative bacterial lipopolysaccharide (LPS) challenge to induce a robust immune and stress response.

Results: Compared to undisturbed controls, MD rats developed significantly more periodontal bone loss as adults, whereas HD rats showed a tendency to less disease. MD and HD rats exhibited depression-like behaviour in a novel open field test, while MD rats showed higher glucocorticoid receptor (Gr) expression in the hippocampus, and HD rats had altered methylation of genes involved in the expression of hippocampal Gr. LPS provoked a significantly lower increase in circulating levels of the cytokine TGF-1β in MD and HD rats, but there were no significant differences in levels of the stress hormone corticosterone.

Conclusion: Stressful environmental exposures in very early life may alter immune responses in a manner that influences susceptibility/resistance to periodontitis.

No MeSH data available.


Related in: MedlinePlus