Limits...
Mechanism of enhanced Bombyx mori nucleopolyhedrovirus-resistance by titanium dioxide nanoparticles in silkworm.

Xu K, Li F, Ma L, Wang B, Zhang H, Ni M, Hong F, Shen W, Li B - PLoS ONE (2015)

Bottom Line: We found that low doses of TiO2 NPs improved the resistance of silkworm against BmNPV by 14.88-fold, with the mortalities of the experimental group and control group being 0.56% and 8.33% at 144 h, respectively.Immune signaling pathway analysis revealed that TiO2 NPs inhibited the proliferation of silkworm BmNPV to reduce the activation levels of janus kinase/signal transducer and activator of transcription (JAK/STAT) and phosphatidylinositol 3-kinase (PI3K)-Akt signaling pathway, while promoting the expression of Bmakt to improve the immunity.Overall, our results demonstrate that TiO2 NPs increase silkworm resistance against BmNPV by inhibiting virus proliferation and improving immunity in silkworms.

View Article: PubMed Central - PubMed

Affiliation: School of Basic Medicine and Biological Sciences, Soochow University, Suzhou, Jiangsu 215123, P.R. China.

ABSTRACT
The infection of Bombyx mori nucleopolyhedrovirus (BmNPV) in silkworms is often lethal. It is difficult to prevent, and its lethality is correlated with both viral particle characteristics and silkworm strains. Low doses of titanium dioxide nanoparticles (TiO2 NPs) can promote silkworm growth and improve its resistance to organophosphate pesticides. In this study, TiO2 NPs' effect on BmNPV resistance was investigated by analyzing the characteristics of BmNPV proliferation and transcriptional differences in silkworm midgut and the transcriptional changes of immunity related genes after feeding with TiO2 NPs. We found that low doses of TiO2 NPs improved the resistance of silkworm against BmNPV by 14.88-fold, with the mortalities of the experimental group and control group being 0.56% and 8.33% at 144 h, respectively. The proliferation of BmNPV in the midgut was significantly increased 72 h after infection in both experimental and control groups; the control group reached the peak at 120 h, while the experimental group took 24 more hours to reach the maximal value that was 12.63 times lower than the control, indicating that TiO2 NPs can inhibit BmNPV proliferation in the midgut. Consistently, the expression of the BmNPV-resistant gene Bmlipase-1 had the same increase pattern as the proliferation changes. Immune signaling pathway analysis revealed that TiO2 NPs inhibited the proliferation of silkworm BmNPV to reduce the activation levels of janus kinase/signal transducer and activator of transcription (JAK/STAT) and phosphatidylinositol 3-kinase (PI3K)-Akt signaling pathway, while promoting the expression of Bmakt to improve the immunity. Overall, our results demonstrate that TiO2 NPs increase silkworm resistance against BmNPV by inhibiting virus proliferation and improving immunity in silkworms.

Show MeSH

Related in: MedlinePlus

Relative copies of gp64.Black and red histograms represent the control group and the experimental group, respectively. X and Y axes represent the time after infection and the relative copy numbers BmNPV gp64, respectively. The bars in the figure with different letters indicate statistically significant differences (p<0.05).
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4333570&req=5

pone.0118222.g005: Relative copies of gp64.Black and red histograms represent the control group and the experimental group, respectively. X and Y axes represent the time after infection and the relative copy numbers BmNPV gp64, respectively. The bars in the figure with different letters indicate statistically significant differences (p<0.05).

Mentions: BmNPV envelope protein gene gp64 showed similar amplification pattern as that of lef-1 (Fig. 5). The relative copys of gp64 of the control group were all higher than those of the experimental group at all periods after BmNPV infection. 24 h and 48 h after the BmNPV infection, the relative copys of gp64 were 2.18- and 1.13-fold of those of the experimental group, respectively. At 72 h, significant differences started to be observed, with the relative copys of gp64 of control group showing 6.98-fold of the experimental group. Similar to the amplification of lef-1, gp64 entered the rapid growth period also from 96 h to 120 h and reached the maximum at 120 h and maintained the level until mounting. In the experimental group, the rapid growth period was from 120 h to 144 h and the maximum value was reached at 144 h; it was reduced at 168 h to only 17.78% of the maximal level; the peak value of the control group was 12.76-fold higher than that of the experimental group.


Mechanism of enhanced Bombyx mori nucleopolyhedrovirus-resistance by titanium dioxide nanoparticles in silkworm.

Xu K, Li F, Ma L, Wang B, Zhang H, Ni M, Hong F, Shen W, Li B - PLoS ONE (2015)

Relative copies of gp64.Black and red histograms represent the control group and the experimental group, respectively. X and Y axes represent the time after infection and the relative copy numbers BmNPV gp64, respectively. The bars in the figure with different letters indicate statistically significant differences (p<0.05).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4333570&req=5

pone.0118222.g005: Relative copies of gp64.Black and red histograms represent the control group and the experimental group, respectively. X and Y axes represent the time after infection and the relative copy numbers BmNPV gp64, respectively. The bars in the figure with different letters indicate statistically significant differences (p<0.05).
Mentions: BmNPV envelope protein gene gp64 showed similar amplification pattern as that of lef-1 (Fig. 5). The relative copys of gp64 of the control group were all higher than those of the experimental group at all periods after BmNPV infection. 24 h and 48 h after the BmNPV infection, the relative copys of gp64 were 2.18- and 1.13-fold of those of the experimental group, respectively. At 72 h, significant differences started to be observed, with the relative copys of gp64 of control group showing 6.98-fold of the experimental group. Similar to the amplification of lef-1, gp64 entered the rapid growth period also from 96 h to 120 h and reached the maximum at 120 h and maintained the level until mounting. In the experimental group, the rapid growth period was from 120 h to 144 h and the maximum value was reached at 144 h; it was reduced at 168 h to only 17.78% of the maximal level; the peak value of the control group was 12.76-fold higher than that of the experimental group.

Bottom Line: We found that low doses of TiO2 NPs improved the resistance of silkworm against BmNPV by 14.88-fold, with the mortalities of the experimental group and control group being 0.56% and 8.33% at 144 h, respectively.Immune signaling pathway analysis revealed that TiO2 NPs inhibited the proliferation of silkworm BmNPV to reduce the activation levels of janus kinase/signal transducer and activator of transcription (JAK/STAT) and phosphatidylinositol 3-kinase (PI3K)-Akt signaling pathway, while promoting the expression of Bmakt to improve the immunity.Overall, our results demonstrate that TiO2 NPs increase silkworm resistance against BmNPV by inhibiting virus proliferation and improving immunity in silkworms.

View Article: PubMed Central - PubMed

Affiliation: School of Basic Medicine and Biological Sciences, Soochow University, Suzhou, Jiangsu 215123, P.R. China.

ABSTRACT
The infection of Bombyx mori nucleopolyhedrovirus (BmNPV) in silkworms is often lethal. It is difficult to prevent, and its lethality is correlated with both viral particle characteristics and silkworm strains. Low doses of titanium dioxide nanoparticles (TiO2 NPs) can promote silkworm growth and improve its resistance to organophosphate pesticides. In this study, TiO2 NPs' effect on BmNPV resistance was investigated by analyzing the characteristics of BmNPV proliferation and transcriptional differences in silkworm midgut and the transcriptional changes of immunity related genes after feeding with TiO2 NPs. We found that low doses of TiO2 NPs improved the resistance of silkworm against BmNPV by 14.88-fold, with the mortalities of the experimental group and control group being 0.56% and 8.33% at 144 h, respectively. The proliferation of BmNPV in the midgut was significantly increased 72 h after infection in both experimental and control groups; the control group reached the peak at 120 h, while the experimental group took 24 more hours to reach the maximal value that was 12.63 times lower than the control, indicating that TiO2 NPs can inhibit BmNPV proliferation in the midgut. Consistently, the expression of the BmNPV-resistant gene Bmlipase-1 had the same increase pattern as the proliferation changes. Immune signaling pathway analysis revealed that TiO2 NPs inhibited the proliferation of silkworm BmNPV to reduce the activation levels of janus kinase/signal transducer and activator of transcription (JAK/STAT) and phosphatidylinositol 3-kinase (PI3K)-Akt signaling pathway, while promoting the expression of Bmakt to improve the immunity. Overall, our results demonstrate that TiO2 NPs increase silkworm resistance against BmNPV by inhibiting virus proliferation and improving immunity in silkworms.

Show MeSH
Related in: MedlinePlus