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Solomonseal polysaccharide and sulfated Codonopsis pilosula polysaccharide synergistically resist Newcastle disease virus.

Liu C, Chen J, Li E, Fan Q, Wang D, Zhang C, Li P, Li X, Chen X, Qiu S, Gao Z, Li H, Hu Y - PLoS ONE (2015)

Bottom Line: The results indicated that SP9-sCP1 presented the lowest morbidity and mortality and the highest cure rate.These results indicate that Solomonseal polysaccharide and sulfated Codonopsis pilosula polysaccharide synergistically resist NDV.Moreover, SP9-sCP1 had the highest efficacy and may be used as a new antiviral drug.

View Article: PubMed Central - PubMed

Affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, PR China.

ABSTRACT
Five combinations of three ratios (PS9-sPS1, PS7-sPS3 and PS6-sPS4) were prepared with polysaccharide (PS) and sulfated polysaccharide (sPS). The antiviral activities of these compounds were subsequently compared in vitro using the MTT assay, observation of the virus structure and immunofluorescence. The results demonstrated that SP9-sCP1, CP7-sCA3, EP7-sAP3, CA9-sEP1 and EP7-sCA3 presented higher activities, and SP9-sCP1 displayed the highest virus inhibition rate and clearly killed the virus and inhibited viral antigen expression. In an in vivo test, 28-day-old chickens were challenged with Newcastle disease virus (NDV) and were administered the five drug combinations. On day 14 after the challenge, the morbidity, mortality and cure rate in each group were calculated. The results indicated that SP9-sCP1 presented the lowest morbidity and mortality and the highest cure rate. These results indicate that Solomonseal polysaccharide and sulfated Codonopsis pilosula polysaccharide synergistically resist NDV. Moreover, SP9-sCP1 had the highest efficacy and may be used as a new antiviral drug.

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Related in: MedlinePlus

The highest virus inhibitory rates in all prescription groups.a-h Bars marked without the same letters differ significantly (P <0.05).
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pone.0117916.g002: The highest virus inhibitory rates in all prescription groups.a-h Bars marked without the same letters differ significantly (P <0.05).

Mentions: The highest virus inhibitory rates of all combinations are illustrated in Fig. 2. For the three ratios of each prescription, the highest virus inhibitory rates were observed in the CP7-sCA3, EP7-sAP3, EP7-sCA3 and SP9-sCP1 groups and were significantly higher than those in other two ratio groups (P <0.05). Among the fifteen combinations, the virus inhibitory rate in SP9-sCP1 group was the highest (66.12%) and significantly higher than those in other fourteen groups (P <0.05). The second highest was the CP7-sCA3 group (52.89%) and was significantly higher than the other thirteen groups (P<0.05), followed by the EP7-sAP3 (42.18%), CA9-sEP1 (41.20%) and EP7-sCA3 (39.0%) groups.


Solomonseal polysaccharide and sulfated Codonopsis pilosula polysaccharide synergistically resist Newcastle disease virus.

Liu C, Chen J, Li E, Fan Q, Wang D, Zhang C, Li P, Li X, Chen X, Qiu S, Gao Z, Li H, Hu Y - PLoS ONE (2015)

The highest virus inhibitory rates in all prescription groups.a-h Bars marked without the same letters differ significantly (P <0.05).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4333568&req=5

pone.0117916.g002: The highest virus inhibitory rates in all prescription groups.a-h Bars marked without the same letters differ significantly (P <0.05).
Mentions: The highest virus inhibitory rates of all combinations are illustrated in Fig. 2. For the three ratios of each prescription, the highest virus inhibitory rates were observed in the CP7-sCA3, EP7-sAP3, EP7-sCA3 and SP9-sCP1 groups and were significantly higher than those in other two ratio groups (P <0.05). Among the fifteen combinations, the virus inhibitory rate in SP9-sCP1 group was the highest (66.12%) and significantly higher than those in other fourteen groups (P <0.05). The second highest was the CP7-sCA3 group (52.89%) and was significantly higher than the other thirteen groups (P<0.05), followed by the EP7-sAP3 (42.18%), CA9-sEP1 (41.20%) and EP7-sCA3 (39.0%) groups.

Bottom Line: The results indicated that SP9-sCP1 presented the lowest morbidity and mortality and the highest cure rate.These results indicate that Solomonseal polysaccharide and sulfated Codonopsis pilosula polysaccharide synergistically resist NDV.Moreover, SP9-sCP1 had the highest efficacy and may be used as a new antiviral drug.

View Article: PubMed Central - PubMed

Affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, PR China.

ABSTRACT
Five combinations of three ratios (PS9-sPS1, PS7-sPS3 and PS6-sPS4) were prepared with polysaccharide (PS) and sulfated polysaccharide (sPS). The antiviral activities of these compounds were subsequently compared in vitro using the MTT assay, observation of the virus structure and immunofluorescence. The results demonstrated that SP9-sCP1, CP7-sCA3, EP7-sAP3, CA9-sEP1 and EP7-sCA3 presented higher activities, and SP9-sCP1 displayed the highest virus inhibition rate and clearly killed the virus and inhibited viral antigen expression. In an in vivo test, 28-day-old chickens were challenged with Newcastle disease virus (NDV) and were administered the five drug combinations. On day 14 after the challenge, the morbidity, mortality and cure rate in each group were calculated. The results indicated that SP9-sCP1 presented the lowest morbidity and mortality and the highest cure rate. These results indicate that Solomonseal polysaccharide and sulfated Codonopsis pilosula polysaccharide synergistically resist NDV. Moreover, SP9-sCP1 had the highest efficacy and may be used as a new antiviral drug.

Show MeSH
Related in: MedlinePlus