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Effectiveness of Ceftriaxone Treatment in Preventing Relapse-like Drinking Behavior Following Long-term Ethanol Dependence in P Rats.

Rao P, Sari Y - J Addict Res Ther (2014)

Bottom Line: Importantly, ceftriaxone treatment at both doses did not cause any significant changes in body weight compared to saline treated group.We report here that ceftriaxone at higher dose has been found to be effective in the attenuation of relapse-like ethanol-drinking behavior in chronic ethanol intake model.This is in accordance with previous data from our lab in cocaine animal model demonstrating that only higher dose of ceftriaxone has been effective in attenuating cocaine relapse.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Pharmacology, College of Pharmacy and Pharmaceutical Sciences, University of Toledo, Toledo, OH, USA.

ABSTRACT

Objective: To evaluate the effectiveness of ceftriaxone treatment in attenuating relapse-like ethanol drinking behavior in male P rats following 14-weeks of continuous ethanol consumption.

Methods: After 14-weeks of continuous access to free choice of 15% and 30% ethanol, male P rats were deprived of ethanol for two weeks. On the last five days of abstinence period, P rats were treated, once a day, with either saline or ceftriaxone (50 or 200 mg/kg; i.p.). This was followed by re-exposure to ethanol for the next 10 days to simulate the relapse-like ethanol drinking behavior.

Results: Ceftriaxone treatment (during abstinence) reduced ethanol intake upon re-exposure to ethanol, compared to the saline treated P rats. This statistically significant reduction in ethanol consumption in P rats following treatment with ceftriaxone (200 mg/kg/day) was observed from Day 2 to Day 9. Similarly, water consumption in P rats treated with ceftriaxone was significantly higher than the saline treated group between Day 2 and Day 7. Importantly, ceftriaxone treatment at both doses did not cause any significant changes in body weight compared to saline treated group.

Conclusions: We report here that ceftriaxone at higher dose has been found to be effective in the attenuation of relapse-like ethanol-drinking behavior in chronic ethanol intake model. This is in accordance with previous data from our lab in cocaine animal model demonstrating that only higher dose of ceftriaxone has been effective in attenuating cocaine relapse.

No MeSH data available.


Related in: MedlinePlus

Graph represents average water intake (ml/kg/day) during the 10 days of re-exposure to ethanol. Based on GLM repeated measures followed by one-way ANOVA, ceftriaxone treatment resulted in significantly higher water consumption compared to saline-treated control group on Days 2 and 5 for 50 mg/kg, and on Days, 2, 3, and 7 for 200 mg/kg. Data are expressed as mean ± SEM (*: p<0.05). Saline group (n=5); ceftriaxone groups (50 and 100 mg/kg, i.p. body weight, n=6 for each group)
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Figure 2: Graph represents average water intake (ml/kg/day) during the 10 days of re-exposure to ethanol. Based on GLM repeated measures followed by one-way ANOVA, ceftriaxone treatment resulted in significantly higher water consumption compared to saline-treated control group on Days 2 and 5 for 50 mg/kg, and on Days, 2, 3, and 7 for 200 mg/kg. Data are expressed as mean ± SEM (*: p<0.05). Saline group (n=5); ceftriaxone groups (50 and 100 mg/kg, i.p. body weight, n=6 for each group)

Mentions: Following treatment with ceftriaxone, P rats consumed significantly higher amounts of water when compared to saline treated animals (Figure 2). A significant main effect of Day was observed [F(1,10)=3.53, p<0.05)] along with a significant Day X Treatment interaction effect [F(2,20)=2.93, p<0.05)]. Interestingly, one-way ANOVA revealed that water consumption was statistically different (p<0.05) in ceftriaxone (50 mg/kg/day) treated group as compared to saline treated group only on Day 2 and Day 5. Alternatively, animals treated with higher dose of ceftriaxone (200 mg/kg/day) consumed significantly higher amounts of water as compared to saline treated group, on Days 2, 3, and 7. These findings suggest that increase in water intake is considered as a compensatory mechanism for the decrease in ethanol intake, which is reflecting the amount body fluid intake.


Effectiveness of Ceftriaxone Treatment in Preventing Relapse-like Drinking Behavior Following Long-term Ethanol Dependence in P Rats.

Rao P, Sari Y - J Addict Res Ther (2014)

Graph represents average water intake (ml/kg/day) during the 10 days of re-exposure to ethanol. Based on GLM repeated measures followed by one-way ANOVA, ceftriaxone treatment resulted in significantly higher water consumption compared to saline-treated control group on Days 2 and 5 for 50 mg/kg, and on Days, 2, 3, and 7 for 200 mg/kg. Data are expressed as mean ± SEM (*: p<0.05). Saline group (n=5); ceftriaxone groups (50 and 100 mg/kg, i.p. body weight, n=6 for each group)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4326063&req=5

Figure 2: Graph represents average water intake (ml/kg/day) during the 10 days of re-exposure to ethanol. Based on GLM repeated measures followed by one-way ANOVA, ceftriaxone treatment resulted in significantly higher water consumption compared to saline-treated control group on Days 2 and 5 for 50 mg/kg, and on Days, 2, 3, and 7 for 200 mg/kg. Data are expressed as mean ± SEM (*: p<0.05). Saline group (n=5); ceftriaxone groups (50 and 100 mg/kg, i.p. body weight, n=6 for each group)
Mentions: Following treatment with ceftriaxone, P rats consumed significantly higher amounts of water when compared to saline treated animals (Figure 2). A significant main effect of Day was observed [F(1,10)=3.53, p<0.05)] along with a significant Day X Treatment interaction effect [F(2,20)=2.93, p<0.05)]. Interestingly, one-way ANOVA revealed that water consumption was statistically different (p<0.05) in ceftriaxone (50 mg/kg/day) treated group as compared to saline treated group only on Day 2 and Day 5. Alternatively, animals treated with higher dose of ceftriaxone (200 mg/kg/day) consumed significantly higher amounts of water as compared to saline treated group, on Days 2, 3, and 7. These findings suggest that increase in water intake is considered as a compensatory mechanism for the decrease in ethanol intake, which is reflecting the amount body fluid intake.

Bottom Line: Importantly, ceftriaxone treatment at both doses did not cause any significant changes in body weight compared to saline treated group.We report here that ceftriaxone at higher dose has been found to be effective in the attenuation of relapse-like ethanol-drinking behavior in chronic ethanol intake model.This is in accordance with previous data from our lab in cocaine animal model demonstrating that only higher dose of ceftriaxone has been effective in attenuating cocaine relapse.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Pharmacology, College of Pharmacy and Pharmaceutical Sciences, University of Toledo, Toledo, OH, USA.

ABSTRACT

Objective: To evaluate the effectiveness of ceftriaxone treatment in attenuating relapse-like ethanol drinking behavior in male P rats following 14-weeks of continuous ethanol consumption.

Methods: After 14-weeks of continuous access to free choice of 15% and 30% ethanol, male P rats were deprived of ethanol for two weeks. On the last five days of abstinence period, P rats were treated, once a day, with either saline or ceftriaxone (50 or 200 mg/kg; i.p.). This was followed by re-exposure to ethanol for the next 10 days to simulate the relapse-like ethanol drinking behavior.

Results: Ceftriaxone treatment (during abstinence) reduced ethanol intake upon re-exposure to ethanol, compared to the saline treated P rats. This statistically significant reduction in ethanol consumption in P rats following treatment with ceftriaxone (200 mg/kg/day) was observed from Day 2 to Day 9. Similarly, water consumption in P rats treated with ceftriaxone was significantly higher than the saline treated group between Day 2 and Day 7. Importantly, ceftriaxone treatment at both doses did not cause any significant changes in body weight compared to saline treated group.

Conclusions: We report here that ceftriaxone at higher dose has been found to be effective in the attenuation of relapse-like ethanol-drinking behavior in chronic ethanol intake model. This is in accordance with previous data from our lab in cocaine animal model demonstrating that only higher dose of ceftriaxone has been effective in attenuating cocaine relapse.

No MeSH data available.


Related in: MedlinePlus