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Effectiveness of Ceftriaxone Treatment in Preventing Relapse-like Drinking Behavior Following Long-term Ethanol Dependence in P Rats.

Rao P, Sari Y - J Addict Res Ther (2014)

Bottom Line: Importantly, ceftriaxone treatment at both doses did not cause any significant changes in body weight compared to saline treated group.We report here that ceftriaxone at higher dose has been found to be effective in the attenuation of relapse-like ethanol-drinking behavior in chronic ethanol intake model.This is in accordance with previous data from our lab in cocaine animal model demonstrating that only higher dose of ceftriaxone has been effective in attenuating cocaine relapse.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Pharmacology, College of Pharmacy and Pharmaceutical Sciences, University of Toledo, Toledo, OH, USA.

ABSTRACT

Objective: To evaluate the effectiveness of ceftriaxone treatment in attenuating relapse-like ethanol drinking behavior in male P rats following 14-weeks of continuous ethanol consumption.

Methods: After 14-weeks of continuous access to free choice of 15% and 30% ethanol, male P rats were deprived of ethanol for two weeks. On the last five days of abstinence period, P rats were treated, once a day, with either saline or ceftriaxone (50 or 200 mg/kg; i.p.). This was followed by re-exposure to ethanol for the next 10 days to simulate the relapse-like ethanol drinking behavior.

Results: Ceftriaxone treatment (during abstinence) reduced ethanol intake upon re-exposure to ethanol, compared to the saline treated P rats. This statistically significant reduction in ethanol consumption in P rats following treatment with ceftriaxone (200 mg/kg/day) was observed from Day 2 to Day 9. Similarly, water consumption in P rats treated with ceftriaxone was significantly higher than the saline treated group between Day 2 and Day 7. Importantly, ceftriaxone treatment at both doses did not cause any significant changes in body weight compared to saline treated group.

Conclusions: We report here that ceftriaxone at higher dose has been found to be effective in the attenuation of relapse-like ethanol-drinking behavior in chronic ethanol intake model. This is in accordance with previous data from our lab in cocaine animal model demonstrating that only higher dose of ceftriaxone has been effective in attenuating cocaine relapse.

No MeSH data available.


Related in: MedlinePlus

Graph represents average ethanol intake (g/kg/day) during the 10 days of re-exposure to ethanol. Based on GLM repeated measures followed by one-way ANOVA, ceftriaxone treatment (200 mg/kg/day) resulted in a significant reduction in ethanol consumption compared to saline vehicle-treated control group from Day 2 to Day 9. Lower dose of ceftriaxone (50 mg/kg/ day) did not cause any reduction in ethanol intake by P rats. Data are expressed as mean ± SEM (*: p<0.05). Saline group (n=5); ceftriaxone groups (50 and 100 mg/kg, i.p. body weight, n=6 for each group)
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Figure 1: Graph represents average ethanol intake (g/kg/day) during the 10 days of re-exposure to ethanol. Based on GLM repeated measures followed by one-way ANOVA, ceftriaxone treatment (200 mg/kg/day) resulted in a significant reduction in ethanol consumption compared to saline vehicle-treated control group from Day 2 to Day 9. Lower dose of ceftriaxone (50 mg/kg/ day) did not cause any reduction in ethanol intake by P rats. Data are expressed as mean ± SEM (*: p<0.05). Saline group (n=5); ceftriaxone groups (50 and 100 mg/kg, i.p. body weight, n=6 for each group)

Mentions: The effect of ceftriaxone treatment on relapse-like ethanol drinking behavior was monitored for 10 days following abstinence. Figure 1 represents the average ethanol consumption by P rats (g/kg/day) treated with either ceftriaxone (50 or 200 mg/kg/day) or saline vehicle. Baseline value represents the average ethanol consumed over the two weeks preceding abstinence (weeks 13 and 14). A GLM repeated measures analysis comparing ethanol consumption after re-exposure between the three groups revealed a significant main effect of Day [F(1,10)=7.65, p<0.05)] along with a significant Day X Treatment interaction effect [F(2,20)=2.11, p<0.05)]. One-way ANOVA, post hoc dunnett’s test, revealed that ceftriaxone treatment (during ethanol deprivation) induced reduction of ethanol intake upon re-exposure to ethanol, compared to saline treated group, was statistically significant (p<0.05) only for the higher dose (200 mg/kg). This effect was observed from Day 2 to Day 9. The lower dose of ceftriaxone used in this study (50 mg/kg/day) did not induce any significant decrease in ethanol consumption. These findings suggest that higher dose, which is known to upregulate GLT1 levels in key reward brain regions, has been effective in reducing ethanol intake.


Effectiveness of Ceftriaxone Treatment in Preventing Relapse-like Drinking Behavior Following Long-term Ethanol Dependence in P Rats.

Rao P, Sari Y - J Addict Res Ther (2014)

Graph represents average ethanol intake (g/kg/day) during the 10 days of re-exposure to ethanol. Based on GLM repeated measures followed by one-way ANOVA, ceftriaxone treatment (200 mg/kg/day) resulted in a significant reduction in ethanol consumption compared to saline vehicle-treated control group from Day 2 to Day 9. Lower dose of ceftriaxone (50 mg/kg/ day) did not cause any reduction in ethanol intake by P rats. Data are expressed as mean ± SEM (*: p<0.05). Saline group (n=5); ceftriaxone groups (50 and 100 mg/kg, i.p. body weight, n=6 for each group)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4326063&req=5

Figure 1: Graph represents average ethanol intake (g/kg/day) during the 10 days of re-exposure to ethanol. Based on GLM repeated measures followed by one-way ANOVA, ceftriaxone treatment (200 mg/kg/day) resulted in a significant reduction in ethanol consumption compared to saline vehicle-treated control group from Day 2 to Day 9. Lower dose of ceftriaxone (50 mg/kg/ day) did not cause any reduction in ethanol intake by P rats. Data are expressed as mean ± SEM (*: p<0.05). Saline group (n=5); ceftriaxone groups (50 and 100 mg/kg, i.p. body weight, n=6 for each group)
Mentions: The effect of ceftriaxone treatment on relapse-like ethanol drinking behavior was monitored for 10 days following abstinence. Figure 1 represents the average ethanol consumption by P rats (g/kg/day) treated with either ceftriaxone (50 or 200 mg/kg/day) or saline vehicle. Baseline value represents the average ethanol consumed over the two weeks preceding abstinence (weeks 13 and 14). A GLM repeated measures analysis comparing ethanol consumption after re-exposure between the three groups revealed a significant main effect of Day [F(1,10)=7.65, p<0.05)] along with a significant Day X Treatment interaction effect [F(2,20)=2.11, p<0.05)]. One-way ANOVA, post hoc dunnett’s test, revealed that ceftriaxone treatment (during ethanol deprivation) induced reduction of ethanol intake upon re-exposure to ethanol, compared to saline treated group, was statistically significant (p<0.05) only for the higher dose (200 mg/kg). This effect was observed from Day 2 to Day 9. The lower dose of ceftriaxone used in this study (50 mg/kg/day) did not induce any significant decrease in ethanol consumption. These findings suggest that higher dose, which is known to upregulate GLT1 levels in key reward brain regions, has been effective in reducing ethanol intake.

Bottom Line: Importantly, ceftriaxone treatment at both doses did not cause any significant changes in body weight compared to saline treated group.We report here that ceftriaxone at higher dose has been found to be effective in the attenuation of relapse-like ethanol-drinking behavior in chronic ethanol intake model.This is in accordance with previous data from our lab in cocaine animal model demonstrating that only higher dose of ceftriaxone has been effective in attenuating cocaine relapse.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Pharmacology, College of Pharmacy and Pharmaceutical Sciences, University of Toledo, Toledo, OH, USA.

ABSTRACT

Objective: To evaluate the effectiveness of ceftriaxone treatment in attenuating relapse-like ethanol drinking behavior in male P rats following 14-weeks of continuous ethanol consumption.

Methods: After 14-weeks of continuous access to free choice of 15% and 30% ethanol, male P rats were deprived of ethanol for two weeks. On the last five days of abstinence period, P rats were treated, once a day, with either saline or ceftriaxone (50 or 200 mg/kg; i.p.). This was followed by re-exposure to ethanol for the next 10 days to simulate the relapse-like ethanol drinking behavior.

Results: Ceftriaxone treatment (during abstinence) reduced ethanol intake upon re-exposure to ethanol, compared to the saline treated P rats. This statistically significant reduction in ethanol consumption in P rats following treatment with ceftriaxone (200 mg/kg/day) was observed from Day 2 to Day 9. Similarly, water consumption in P rats treated with ceftriaxone was significantly higher than the saline treated group between Day 2 and Day 7. Importantly, ceftriaxone treatment at both doses did not cause any significant changes in body weight compared to saline treated group.

Conclusions: We report here that ceftriaxone at higher dose has been found to be effective in the attenuation of relapse-like ethanol-drinking behavior in chronic ethanol intake model. This is in accordance with previous data from our lab in cocaine animal model demonstrating that only higher dose of ceftriaxone has been effective in attenuating cocaine relapse.

No MeSH data available.


Related in: MedlinePlus