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Development of noncytotoxic chitosan-gold nanocomposites as efficient antibacterial materials.

Regiel-Futyra A, Kus-Liśkiewicz M, Sebastian V, Irusta S, Arruebo M, Stochel G, Kyzioł A - ACS Appl Mater Interfaces (2015)

Bottom Line: The resulting nanocomposites did not show any cytotoxicity against mammalian somatic and tumoral cells.They produced a disruptive effect on the bacteria wall while their internalization was hindered on the eukaryotic cells.This selectivity and safety make them potentially applicable as antimicrobial coatings in the biomedical field.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Chemistry, Jagiellonian University , Ingardena 3, 30-060 Kraków, Poland.

ABSTRACT
This work describes the synthesis and characterization of noncytotoxic nanocomposites either colloidal or as films exhibiting high antibacterial activity. The biocompatible and biodegradable polymer chitosan was used as reducing and stabilizing agent for the synthesis of gold nanoparticles embedded in it. Herein, for the first time, three different chitosan grades varying in the average molecular weight and deacetylation degree (DD) were used with an optimized gold precursor concentration. Several factors were analyzed in order to obtain antimicrobial but not cytotoxic nanocomposite materials. Films based on chitosan with medium molecular weight and the highest DD exhibited the highest antibacterial activity against biofilm forming strains of Staphylococcus aureus and Pseudomonas aeruginosa. The resulting nanocomposites did not show any cytotoxicity against mammalian somatic and tumoral cells. They produced a disruptive effect on the bacteria wall while their internalization was hindered on the eukaryotic cells. This selectivity and safety make them potentially applicable as antimicrobial coatings in the biomedical field.

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Cellularviability after incubation with different CS_L (A), CS_M(B), CS_H (C) based AuNP colloid concentrations for A549 (I, MTT assay)and HaCaT (II, LDH assay; III, MTT assay) cell line. Data are expressedas the mean ± standard error (n = 9).
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fig9: Cellularviability after incubation with different CS_L (A), CS_M(B), CS_H (C) based AuNP colloid concentrations for A549 (I, MTT assay)and HaCaT (II, LDH assay; III, MTT assay) cell line. Data are expressedas the mean ± standard error (n = 9).

Mentions: MTT and LDH assays were carriedout to assess the effect of chitosan–gold nanocomposites onmammalian cell viability. Any possible interference of the nanoparticleswith the colorimetric tests was discarded.73 The cytotoxicity of the prepared materials was evaluated after 24h of incubation for both colloids based on gold nanoparticles embeddedin chitosan and solid nanocomposites. Figure 9 presents the data for A549 cells, showing a slight and concentration-dependentdecrease in cell viability assessed by the MTT test. Increasing inthe range from 143 μM up to 714 μM, the most significantcytotoxic effect can be observed for AuNPs based on chitosan withthe highest Mw, where the cell population reduction reaches almosta 45%. Similarly, for CS_L based samples, cellular viability decreasedto a 69% for the highest concentration tested. The lowest cell populationreduction (<18%) is observed for chitosan with the medium Mw.


Development of noncytotoxic chitosan-gold nanocomposites as efficient antibacterial materials.

Regiel-Futyra A, Kus-Liśkiewicz M, Sebastian V, Irusta S, Arruebo M, Stochel G, Kyzioł A - ACS Appl Mater Interfaces (2015)

Cellularviability after incubation with different CS_L (A), CS_M(B), CS_H (C) based AuNP colloid concentrations for A549 (I, MTT assay)and HaCaT (II, LDH assay; III, MTT assay) cell line. Data are expressedas the mean ± standard error (n = 9).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4326049&req=5

fig9: Cellularviability after incubation with different CS_L (A), CS_M(B), CS_H (C) based AuNP colloid concentrations for A549 (I, MTT assay)and HaCaT (II, LDH assay; III, MTT assay) cell line. Data are expressedas the mean ± standard error (n = 9).
Mentions: MTT and LDH assays were carriedout to assess the effect of chitosan–gold nanocomposites onmammalian cell viability. Any possible interference of the nanoparticleswith the colorimetric tests was discarded.73 The cytotoxicity of the prepared materials was evaluated after 24h of incubation for both colloids based on gold nanoparticles embeddedin chitosan and solid nanocomposites. Figure 9 presents the data for A549 cells, showing a slight and concentration-dependentdecrease in cell viability assessed by the MTT test. Increasing inthe range from 143 μM up to 714 μM, the most significantcytotoxic effect can be observed for AuNPs based on chitosan withthe highest Mw, where the cell population reduction reaches almosta 45%. Similarly, for CS_L based samples, cellular viability decreasedto a 69% for the highest concentration tested. The lowest cell populationreduction (<18%) is observed for chitosan with the medium Mw.

Bottom Line: The resulting nanocomposites did not show any cytotoxicity against mammalian somatic and tumoral cells.They produced a disruptive effect on the bacteria wall while their internalization was hindered on the eukaryotic cells.This selectivity and safety make them potentially applicable as antimicrobial coatings in the biomedical field.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Chemistry, Jagiellonian University , Ingardena 3, 30-060 Kraków, Poland.

ABSTRACT
This work describes the synthesis and characterization of noncytotoxic nanocomposites either colloidal or as films exhibiting high antibacterial activity. The biocompatible and biodegradable polymer chitosan was used as reducing and stabilizing agent for the synthesis of gold nanoparticles embedded in it. Herein, for the first time, three different chitosan grades varying in the average molecular weight and deacetylation degree (DD) were used with an optimized gold precursor concentration. Several factors were analyzed in order to obtain antimicrobial but not cytotoxic nanocomposite materials. Films based on chitosan with medium molecular weight and the highest DD exhibited the highest antibacterial activity against biofilm forming strains of Staphylococcus aureus and Pseudomonas aeruginosa. The resulting nanocomposites did not show any cytotoxicity against mammalian somatic and tumoral cells. They produced a disruptive effect on the bacteria wall while their internalization was hindered on the eukaryotic cells. This selectivity and safety make them potentially applicable as antimicrobial coatings in the biomedical field.

Show MeSH
Related in: MedlinePlus