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Development of noncytotoxic chitosan-gold nanocomposites as efficient antibacterial materials.

Regiel-Futyra A, Kus-Liśkiewicz M, Sebastian V, Irusta S, Arruebo M, Stochel G, Kyzioł A - ACS Appl Mater Interfaces (2015)

Bottom Line: The resulting nanocomposites did not show any cytotoxicity against mammalian somatic and tumoral cells.They produced a disruptive effect on the bacteria wall while their internalization was hindered on the eukaryotic cells.This selectivity and safety make them potentially applicable as antimicrobial coatings in the biomedical field.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Chemistry, Jagiellonian University , Ingardena 3, 30-060 Kraków, Poland.

ABSTRACT
This work describes the synthesis and characterization of noncytotoxic nanocomposites either colloidal or as films exhibiting high antibacterial activity. The biocompatible and biodegradable polymer chitosan was used as reducing and stabilizing agent for the synthesis of gold nanoparticles embedded in it. Herein, for the first time, three different chitosan grades varying in the average molecular weight and deacetylation degree (DD) were used with an optimized gold precursor concentration. Several factors were analyzed in order to obtain antimicrobial but not cytotoxic nanocomposite materials. Films based on chitosan with medium molecular weight and the highest DD exhibited the highest antibacterial activity against biofilm forming strains of Staphylococcus aureus and Pseudomonas aeruginosa. The resulting nanocomposites did not show any cytotoxicity against mammalian somatic and tumoral cells. They produced a disruptive effect on the bacteria wall while their internalization was hindered on the eukaryotic cells. This selectivity and safety make them potentially applicable as antimicrobial coatings in the biomedical field.

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Related in: MedlinePlus

Antibacterial test results (Standard Norm ASTME2180-07) for CS_L/M/Hcomposites with different based AuNPs loading (1, 2, 5 and 10 mM goldprecursor), against S. aureus ATCC25923 (A) and P. aeruginosa ATCC 27853(B). Data were expressed as the mean ± standard error (n = 3).
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fig7: Antibacterial test results (Standard Norm ASTME2180-07) for CS_L/M/Hcomposites with different based AuNPs loading (1, 2, 5 and 10 mM goldprecursor), against S. aureus ATCC25923 (A) and P. aeruginosa ATCC 27853(B). Data were expressed as the mean ± standard error (n = 3).

Mentions: To determine the biocidalpotential of CS-AuNP films, two representative bacterial strains wereselected. Both of them, S. aureus ATTC25923 and P. aeruginosa ATTC 27853,normally populate the skin or mucous membranes of humans and causea wide range of serious diseases.65,66 The antibacterialactivity of gold nanoparticles embedded within the chitosan filmswas tested according to the Standard Norm ASTM E2180-07 for polymericsubstances. Composites were sterilized before the antibacterial test,according to the norm demands. To certify the reproducibility of antibacterialtests, experiments were performed in triplicate. The test resultswere calculated as CFU/mL and are presented in Figure 7 (Ct0 stands for initial bacterial culture at thebeginning of the experiment).


Development of noncytotoxic chitosan-gold nanocomposites as efficient antibacterial materials.

Regiel-Futyra A, Kus-Liśkiewicz M, Sebastian V, Irusta S, Arruebo M, Stochel G, Kyzioł A - ACS Appl Mater Interfaces (2015)

Antibacterial test results (Standard Norm ASTME2180-07) for CS_L/M/Hcomposites with different based AuNPs loading (1, 2, 5 and 10 mM goldprecursor), against S. aureus ATCC25923 (A) and P. aeruginosa ATCC 27853(B). Data were expressed as the mean ± standard error (n = 3).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4326049&req=5

fig7: Antibacterial test results (Standard Norm ASTME2180-07) for CS_L/M/Hcomposites with different based AuNPs loading (1, 2, 5 and 10 mM goldprecursor), against S. aureus ATCC25923 (A) and P. aeruginosa ATCC 27853(B). Data were expressed as the mean ± standard error (n = 3).
Mentions: To determine the biocidalpotential of CS-AuNP films, two representative bacterial strains wereselected. Both of them, S. aureus ATTC25923 and P. aeruginosa ATTC 27853,normally populate the skin or mucous membranes of humans and causea wide range of serious diseases.65,66 The antibacterialactivity of gold nanoparticles embedded within the chitosan filmswas tested according to the Standard Norm ASTM E2180-07 for polymericsubstances. Composites were sterilized before the antibacterial test,according to the norm demands. To certify the reproducibility of antibacterialtests, experiments were performed in triplicate. The test resultswere calculated as CFU/mL and are presented in Figure 7 (Ct0 stands for initial bacterial culture at thebeginning of the experiment).

Bottom Line: The resulting nanocomposites did not show any cytotoxicity against mammalian somatic and tumoral cells.They produced a disruptive effect on the bacteria wall while their internalization was hindered on the eukaryotic cells.This selectivity and safety make them potentially applicable as antimicrobial coatings in the biomedical field.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Chemistry, Jagiellonian University , Ingardena 3, 30-060 Kraków, Poland.

ABSTRACT
This work describes the synthesis and characterization of noncytotoxic nanocomposites either colloidal or as films exhibiting high antibacterial activity. The biocompatible and biodegradable polymer chitosan was used as reducing and stabilizing agent for the synthesis of gold nanoparticles embedded in it. Herein, for the first time, three different chitosan grades varying in the average molecular weight and deacetylation degree (DD) were used with an optimized gold precursor concentration. Several factors were analyzed in order to obtain antimicrobial but not cytotoxic nanocomposite materials. Films based on chitosan with medium molecular weight and the highest DD exhibited the highest antibacterial activity against biofilm forming strains of Staphylococcus aureus and Pseudomonas aeruginosa. The resulting nanocomposites did not show any cytotoxicity against mammalian somatic and tumoral cells. They produced a disruptive effect on the bacteria wall while their internalization was hindered on the eukaryotic cells. This selectivity and safety make them potentially applicable as antimicrobial coatings in the biomedical field.

Show MeSH
Related in: MedlinePlus