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Development of noncytotoxic chitosan-gold nanocomposites as efficient antibacterial materials.

Regiel-Futyra A, Kus-Liśkiewicz M, Sebastian V, Irusta S, Arruebo M, Stochel G, Kyzioł A - ACS Appl Mater Interfaces (2015)

Bottom Line: The resulting nanocomposites did not show any cytotoxicity against mammalian somatic and tumoral cells.They produced a disruptive effect on the bacteria wall while their internalization was hindered on the eukaryotic cells.This selectivity and safety make them potentially applicable as antimicrobial coatings in the biomedical field.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Chemistry, Jagiellonian University , Ingardena 3, 30-060 Kraków, Poland.

ABSTRACT
This work describes the synthesis and characterization of noncytotoxic nanocomposites either colloidal or as films exhibiting high antibacterial activity. The biocompatible and biodegradable polymer chitosan was used as reducing and stabilizing agent for the synthesis of gold nanoparticles embedded in it. Herein, for the first time, three different chitosan grades varying in the average molecular weight and deacetylation degree (DD) were used with an optimized gold precursor concentration. Several factors were analyzed in order to obtain antimicrobial but not cytotoxic nanocomposite materials. Films based on chitosan with medium molecular weight and the highest DD exhibited the highest antibacterial activity against biofilm forming strains of Staphylococcus aureus and Pseudomonas aeruginosa. The resulting nanocomposites did not show any cytotoxicity against mammalian somatic and tumoral cells. They produced a disruptive effect on the bacteria wall while their internalization was hindered on the eukaryotic cells. This selectivity and safety make them potentially applicable as antimicrobial coatings in the biomedical field.

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XPS depth profiling resultsof chitosan–gold films (A);XPS maps of AuNPs distribution for M5. The lighter the blue color,the higher the Au concentration present (B) and M10 (C) film. Thelighter the blue color, the higher the Au concentration present.
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fig6: XPS depth profiling resultsof chitosan–gold films (A);XPS maps of AuNPs distribution for M5. The lighter the blue color,the higher the Au concentration present (B) and M10 (C) film. Thelighter the blue color, the higher the Au concentration present.

Mentions: Figure 6A presentsthe Au/C atomic ratio course upon different ion bombardment timesfor chitosan M films with two of the highest gold contents (M5 andM10). The low gold values observed on the surface could be due tothe absence of gold nanoparticles on the surface, but it could alsobe produced by the unavoidable atmospheric contamination consistingmainly of carbon and oxygen. Another explanation for the low goldsurface concentration could be the XPS analysis conditions, the samplesare dried at very low pressure and it could cause the shrinking ofthe polymer chains on the surface encapsulating the gold nanoparticles.After etching, a few layers of the film were removed on sample M5(≈20 nm) and the gold concentration remained constant, indicatinga proper dispersion of the nanoparticles along the film depth. Accordingto the Au/C ratio values for the M10 sample, the thickness showinga gold gradient concentration is thicker, around 80 nm. XPS maps ofthe surface of the films show a homogeneous gold nanoparticle distributionin both samples (Figure 6B,C).


Development of noncytotoxic chitosan-gold nanocomposites as efficient antibacterial materials.

Regiel-Futyra A, Kus-Liśkiewicz M, Sebastian V, Irusta S, Arruebo M, Stochel G, Kyzioł A - ACS Appl Mater Interfaces (2015)

XPS depth profiling resultsof chitosan–gold films (A);XPS maps of AuNPs distribution for M5. The lighter the blue color,the higher the Au concentration present (B) and M10 (C) film. Thelighter the blue color, the higher the Au concentration present.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4326049&req=5

fig6: XPS depth profiling resultsof chitosan–gold films (A);XPS maps of AuNPs distribution for M5. The lighter the blue color,the higher the Au concentration present (B) and M10 (C) film. Thelighter the blue color, the higher the Au concentration present.
Mentions: Figure 6A presentsthe Au/C atomic ratio course upon different ion bombardment timesfor chitosan M films with two of the highest gold contents (M5 andM10). The low gold values observed on the surface could be due tothe absence of gold nanoparticles on the surface, but it could alsobe produced by the unavoidable atmospheric contamination consistingmainly of carbon and oxygen. Another explanation for the low goldsurface concentration could be the XPS analysis conditions, the samplesare dried at very low pressure and it could cause the shrinking ofthe polymer chains on the surface encapsulating the gold nanoparticles.After etching, a few layers of the film were removed on sample M5(≈20 nm) and the gold concentration remained constant, indicatinga proper dispersion of the nanoparticles along the film depth. Accordingto the Au/C ratio values for the M10 sample, the thickness showinga gold gradient concentration is thicker, around 80 nm. XPS maps ofthe surface of the films show a homogeneous gold nanoparticle distributionin both samples (Figure 6B,C).

Bottom Line: The resulting nanocomposites did not show any cytotoxicity against mammalian somatic and tumoral cells.They produced a disruptive effect on the bacteria wall while their internalization was hindered on the eukaryotic cells.This selectivity and safety make them potentially applicable as antimicrobial coatings in the biomedical field.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Chemistry, Jagiellonian University , Ingardena 3, 30-060 Kraków, Poland.

ABSTRACT
This work describes the synthesis and characterization of noncytotoxic nanocomposites either colloidal or as films exhibiting high antibacterial activity. The biocompatible and biodegradable polymer chitosan was used as reducing and stabilizing agent for the synthesis of gold nanoparticles embedded in it. Herein, for the first time, three different chitosan grades varying in the average molecular weight and deacetylation degree (DD) were used with an optimized gold precursor concentration. Several factors were analyzed in order to obtain antimicrobial but not cytotoxic nanocomposite materials. Films based on chitosan with medium molecular weight and the highest DD exhibited the highest antibacterial activity against biofilm forming strains of Staphylococcus aureus and Pseudomonas aeruginosa. The resulting nanocomposites did not show any cytotoxicity against mammalian somatic and tumoral cells. They produced a disruptive effect on the bacteria wall while their internalization was hindered on the eukaryotic cells. This selectivity and safety make them potentially applicable as antimicrobial coatings in the biomedical field.

Show MeSH
Related in: MedlinePlus