Limits...
Development of noncytotoxic chitosan-gold nanocomposites as efficient antibacterial materials.

Regiel-Futyra A, Kus-Liśkiewicz M, Sebastian V, Irusta S, Arruebo M, Stochel G, Kyzioł A - ACS Appl Mater Interfaces (2015)

Bottom Line: The resulting nanocomposites did not show any cytotoxicity against mammalian somatic and tumoral cells.They produced a disruptive effect on the bacteria wall while their internalization was hindered on the eukaryotic cells.This selectivity and safety make them potentially applicable as antimicrobial coatings in the biomedical field.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Chemistry, Jagiellonian University , Ingardena 3, 30-060 Kraków, Poland.

ABSTRACT
This work describes the synthesis and characterization of noncytotoxic nanocomposites either colloidal or as films exhibiting high antibacterial activity. The biocompatible and biodegradable polymer chitosan was used as reducing and stabilizing agent for the synthesis of gold nanoparticles embedded in it. Herein, for the first time, three different chitosan grades varying in the average molecular weight and deacetylation degree (DD) were used with an optimized gold precursor concentration. Several factors were analyzed in order to obtain antimicrobial but not cytotoxic nanocomposite materials. Films based on chitosan with medium molecular weight and the highest DD exhibited the highest antibacterial activity against biofilm forming strains of Staphylococcus aureus and Pseudomonas aeruginosa. The resulting nanocomposites did not show any cytotoxicity against mammalian somatic and tumoral cells. They produced a disruptive effect on the bacteria wall while their internalization was hindered on the eukaryotic cells. This selectivity and safety make them potentially applicable as antimicrobial coatings in the biomedical field.

Show MeSH

Related in: MedlinePlus

FTIR spectra of purechitosan films (L/M/H) and their nanocompositeswith increased gold NP content.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4326049&req=5

fig5: FTIR spectra of purechitosan films (L/M/H) and their nanocompositeswith increased gold NP content.

Mentions: To confirm the specificinteraction of chitosan functional groupswith the metal surface FTIR spectra of pure chitosan films (L/M/H)and chitosan–gold nanocomposites were collected. For betterinterpretation, only the region between 1200 and 1750 cm–1 is presented. Figure 5 shows representativespectra with the characteristic vibrational bands of chitosan. A typicalchitosan spectrum presents bands at ∼1650 and ∼1590cm–1 corresponding to amide I groups, C–Ostretching along with N–H deformation mode (acetylated amine,and to free amine groups, respectively).54 Absorption at 1376 and 1409 cm–1 could be assignedto bending vibrations of −CH2 and −CH3, respectively.61 Also, 1320 and1259 cm–1 bands can be distinguished, corresponding,respectively, to CH2 wagging vibration in primary alcoholand the amide III vibration coming from combination of N–Hdeformation and C–N stretching.


Development of noncytotoxic chitosan-gold nanocomposites as efficient antibacterial materials.

Regiel-Futyra A, Kus-Liśkiewicz M, Sebastian V, Irusta S, Arruebo M, Stochel G, Kyzioł A - ACS Appl Mater Interfaces (2015)

FTIR spectra of purechitosan films (L/M/H) and their nanocompositeswith increased gold NP content.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4326049&req=5

fig5: FTIR spectra of purechitosan films (L/M/H) and their nanocompositeswith increased gold NP content.
Mentions: To confirm the specificinteraction of chitosan functional groupswith the metal surface FTIR spectra of pure chitosan films (L/M/H)and chitosan–gold nanocomposites were collected. For betterinterpretation, only the region between 1200 and 1750 cm–1 is presented. Figure 5 shows representativespectra with the characteristic vibrational bands of chitosan. A typicalchitosan spectrum presents bands at ∼1650 and ∼1590cm–1 corresponding to amide I groups, C–Ostretching along with N–H deformation mode (acetylated amine,and to free amine groups, respectively).54 Absorption at 1376 and 1409 cm–1 could be assignedto bending vibrations of −CH2 and −CH3, respectively.61 Also, 1320 and1259 cm–1 bands can be distinguished, corresponding,respectively, to CH2 wagging vibration in primary alcoholand the amide III vibration coming from combination of N–Hdeformation and C–N stretching.

Bottom Line: The resulting nanocomposites did not show any cytotoxicity against mammalian somatic and tumoral cells.They produced a disruptive effect on the bacteria wall while their internalization was hindered on the eukaryotic cells.This selectivity and safety make them potentially applicable as antimicrobial coatings in the biomedical field.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Chemistry, Jagiellonian University , Ingardena 3, 30-060 Kraków, Poland.

ABSTRACT
This work describes the synthesis and characterization of noncytotoxic nanocomposites either colloidal or as films exhibiting high antibacterial activity. The biocompatible and biodegradable polymer chitosan was used as reducing and stabilizing agent for the synthesis of gold nanoparticles embedded in it. Herein, for the first time, three different chitosan grades varying in the average molecular weight and deacetylation degree (DD) were used with an optimized gold precursor concentration. Several factors were analyzed in order to obtain antimicrobial but not cytotoxic nanocomposite materials. Films based on chitosan with medium molecular weight and the highest DD exhibited the highest antibacterial activity against biofilm forming strains of Staphylococcus aureus and Pseudomonas aeruginosa. The resulting nanocomposites did not show any cytotoxicity against mammalian somatic and tumoral cells. They produced a disruptive effect on the bacteria wall while their internalization was hindered on the eukaryotic cells. This selectivity and safety make them potentially applicable as antimicrobial coatings in the biomedical field.

Show MeSH
Related in: MedlinePlus