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Development of noncytotoxic chitosan-gold nanocomposites as efficient antibacterial materials.

Regiel-Futyra A, Kus-Liśkiewicz M, Sebastian V, Irusta S, Arruebo M, Stochel G, Kyzioł A - ACS Appl Mater Interfaces (2015)

Bottom Line: The resulting nanocomposites did not show any cytotoxicity against mammalian somatic and tumoral cells.They produced a disruptive effect on the bacteria wall while their internalization was hindered on the eukaryotic cells.This selectivity and safety make them potentially applicable as antimicrobial coatings in the biomedical field.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Chemistry, Jagiellonian University , Ingardena 3, 30-060 Kraków, Poland.

ABSTRACT
This work describes the synthesis and characterization of noncytotoxic nanocomposites either colloidal or as films exhibiting high antibacterial activity. The biocompatible and biodegradable polymer chitosan was used as reducing and stabilizing agent for the synthesis of gold nanoparticles embedded in it. Herein, for the first time, three different chitosan grades varying in the average molecular weight and deacetylation degree (DD) were used with an optimized gold precursor concentration. Several factors were analyzed in order to obtain antimicrobial but not cytotoxic nanocomposite materials. Films based on chitosan with medium molecular weight and the highest DD exhibited the highest antibacterial activity against biofilm forming strains of Staphylococcus aureus and Pseudomonas aeruginosa. The resulting nanocomposites did not show any cytotoxicity against mammalian somatic and tumoral cells. They produced a disruptive effect on the bacteria wall while their internalization was hindered on the eukaryotic cells. This selectivity and safety make them potentially applicable as antimicrobial coatings in the biomedical field.

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Related in: MedlinePlus

TEM and STEM-HAADFmicrographs for L1/M1/H1 nanocomposites (A)and M5 (B and C) nanocomposite reveals a proper gold nanoparticlesdistribution across the film.
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fig4: TEM and STEM-HAADFmicrographs for L1/M1/H1 nanocomposites (A)and M5 (B and C) nanocomposite reveals a proper gold nanoparticlesdistribution across the film.

Mentions: To get an insight into the uniformity of the AuNPdistribution among the nanocomposites, TEM analysis of the films withthe lowest gold content was performed. The most uniform AuNP distributionwas observed for CS_M based samples (Figure 4AM1). Unlike M1, unequal layout is apparent for chitosan with thehighest molecular weight (H1) where many areas lacking NPs or showinglarge NP based aggregates are present. Although aggregates were notobserved for sample L1, the distribution of nanoparticles is lessuniform than for the M1 sample. A homogeneous dispersion of AuNPswas also presented for CS_M samples with higher gold content, thusconfirming a high stabilizing potential of chitosan with the mediumaverage molecular weight (Figure 4B). Also,STEM-HAADF micrographs collected for this M5 sample presented goldnanoparticles as bright dots because the contrast is directly relatedto the atomic number, certifying the gold homogeneity when using chitosanmedium based materials (Figure 4C).


Development of noncytotoxic chitosan-gold nanocomposites as efficient antibacterial materials.

Regiel-Futyra A, Kus-Liśkiewicz M, Sebastian V, Irusta S, Arruebo M, Stochel G, Kyzioł A - ACS Appl Mater Interfaces (2015)

TEM and STEM-HAADFmicrographs for L1/M1/H1 nanocomposites (A)and M5 (B and C) nanocomposite reveals a proper gold nanoparticlesdistribution across the film.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4326049&req=5

fig4: TEM and STEM-HAADFmicrographs for L1/M1/H1 nanocomposites (A)and M5 (B and C) nanocomposite reveals a proper gold nanoparticlesdistribution across the film.
Mentions: To get an insight into the uniformity of the AuNPdistribution among the nanocomposites, TEM analysis of the films withthe lowest gold content was performed. The most uniform AuNP distributionwas observed for CS_M based samples (Figure 4AM1). Unlike M1, unequal layout is apparent for chitosan with thehighest molecular weight (H1) where many areas lacking NPs or showinglarge NP based aggregates are present. Although aggregates were notobserved for sample L1, the distribution of nanoparticles is lessuniform than for the M1 sample. A homogeneous dispersion of AuNPswas also presented for CS_M samples with higher gold content, thusconfirming a high stabilizing potential of chitosan with the mediumaverage molecular weight (Figure 4B). Also,STEM-HAADF micrographs collected for this M5 sample presented goldnanoparticles as bright dots because the contrast is directly relatedto the atomic number, certifying the gold homogeneity when using chitosanmedium based materials (Figure 4C).

Bottom Line: The resulting nanocomposites did not show any cytotoxicity against mammalian somatic and tumoral cells.They produced a disruptive effect on the bacteria wall while their internalization was hindered on the eukaryotic cells.This selectivity and safety make them potentially applicable as antimicrobial coatings in the biomedical field.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Chemistry, Jagiellonian University , Ingardena 3, 30-060 Kraków, Poland.

ABSTRACT
This work describes the synthesis and characterization of noncytotoxic nanocomposites either colloidal or as films exhibiting high antibacterial activity. The biocompatible and biodegradable polymer chitosan was used as reducing and stabilizing agent for the synthesis of gold nanoparticles embedded in it. Herein, for the first time, three different chitosan grades varying in the average molecular weight and deacetylation degree (DD) were used with an optimized gold precursor concentration. Several factors were analyzed in order to obtain antimicrobial but not cytotoxic nanocomposite materials. Films based on chitosan with medium molecular weight and the highest DD exhibited the highest antibacterial activity against biofilm forming strains of Staphylococcus aureus and Pseudomonas aeruginosa. The resulting nanocomposites did not show any cytotoxicity against mammalian somatic and tumoral cells. They produced a disruptive effect on the bacteria wall while their internalization was hindered on the eukaryotic cells. This selectivity and safety make them potentially applicable as antimicrobial coatings in the biomedical field.

Show MeSH
Related in: MedlinePlus