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Development of noncytotoxic chitosan-gold nanocomposites as efficient antibacterial materials.

Regiel-Futyra A, Kus-Liśkiewicz M, Sebastian V, Irusta S, Arruebo M, Stochel G, Kyzioł A - ACS Appl Mater Interfaces (2015)

Bottom Line: The resulting nanocomposites did not show any cytotoxicity against mammalian somatic and tumoral cells.They produced a disruptive effect on the bacteria wall while their internalization was hindered on the eukaryotic cells.This selectivity and safety make them potentially applicable as antimicrobial coatings in the biomedical field.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Chemistry, Jagiellonian University , Ingardena 3, 30-060 Kraków, Poland.

ABSTRACT
This work describes the synthesis and characterization of noncytotoxic nanocomposites either colloidal or as films exhibiting high antibacterial activity. The biocompatible and biodegradable polymer chitosan was used as reducing and stabilizing agent for the synthesis of gold nanoparticles embedded in it. Herein, for the first time, three different chitosan grades varying in the average molecular weight and deacetylation degree (DD) were used with an optimized gold precursor concentration. Several factors were analyzed in order to obtain antimicrobial but not cytotoxic nanocomposite materials. Films based on chitosan with medium molecular weight and the highest DD exhibited the highest antibacterial activity against biofilm forming strains of Staphylococcus aureus and Pseudomonas aeruginosa. The resulting nanocomposites did not show any cytotoxicity against mammalian somatic and tumoral cells. They produced a disruptive effect on the bacteria wall while their internalization was hindered on the eukaryotic cells. This selectivity and safety make them potentially applicable as antimicrobial coatings in the biomedical field.

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UV–vis absorptionspectra for CS_L (A), CS_M (B), CS_H (C)based AuNP synthesis progress (1 mM precursor). Spectra were alsocollected after synthesis for all gold initial concentrations: CS_L(D), CS_M (E), CS_H (F).
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fig2: UV–vis absorptionspectra for CS_L (A), CS_M (B), CS_H (C)based AuNP synthesis progress (1 mM precursor). Spectra were alsocollected after synthesis for all gold initial concentrations: CS_L(D), CS_M (E), CS_H (F).

Mentions: Due to the localized surfaceplasmon resonance (SPR) effect coming from the excitation of the conductionelectrons in the metals, the progress of the AuNP synthesis was trackedby using UV–Vis spectroscopy. The measurements were conductedsimultaneously during 8 h for the CS L/M/H AuNPs (1 mM precursor)synthesis (Figure 2A–C).


Development of noncytotoxic chitosan-gold nanocomposites as efficient antibacterial materials.

Regiel-Futyra A, Kus-Liśkiewicz M, Sebastian V, Irusta S, Arruebo M, Stochel G, Kyzioł A - ACS Appl Mater Interfaces (2015)

UV–vis absorptionspectra for CS_L (A), CS_M (B), CS_H (C)based AuNP synthesis progress (1 mM precursor). Spectra were alsocollected after synthesis for all gold initial concentrations: CS_L(D), CS_M (E), CS_H (F).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4326049&req=5

fig2: UV–vis absorptionspectra for CS_L (A), CS_M (B), CS_H (C)based AuNP synthesis progress (1 mM precursor). Spectra were alsocollected after synthesis for all gold initial concentrations: CS_L(D), CS_M (E), CS_H (F).
Mentions: Due to the localized surfaceplasmon resonance (SPR) effect coming from the excitation of the conductionelectrons in the metals, the progress of the AuNP synthesis was trackedby using UV–Vis spectroscopy. The measurements were conductedsimultaneously during 8 h for the CS L/M/H AuNPs (1 mM precursor)synthesis (Figure 2A–C).

Bottom Line: The resulting nanocomposites did not show any cytotoxicity against mammalian somatic and tumoral cells.They produced a disruptive effect on the bacteria wall while their internalization was hindered on the eukaryotic cells.This selectivity and safety make them potentially applicable as antimicrobial coatings in the biomedical field.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Chemistry, Jagiellonian University , Ingardena 3, 30-060 Kraków, Poland.

ABSTRACT
This work describes the synthesis and characterization of noncytotoxic nanocomposites either colloidal or as films exhibiting high antibacterial activity. The biocompatible and biodegradable polymer chitosan was used as reducing and stabilizing agent for the synthesis of gold nanoparticles embedded in it. Herein, for the first time, three different chitosan grades varying in the average molecular weight and deacetylation degree (DD) were used with an optimized gold precursor concentration. Several factors were analyzed in order to obtain antimicrobial but not cytotoxic nanocomposite materials. Films based on chitosan with medium molecular weight and the highest DD exhibited the highest antibacterial activity against biofilm forming strains of Staphylococcus aureus and Pseudomonas aeruginosa. The resulting nanocomposites did not show any cytotoxicity against mammalian somatic and tumoral cells. They produced a disruptive effect on the bacteria wall while their internalization was hindered on the eukaryotic cells. This selectivity and safety make them potentially applicable as antimicrobial coatings in the biomedical field.

Show MeSH
Related in: MedlinePlus