Limits...
Development of noncytotoxic chitosan-gold nanocomposites as efficient antibacterial materials.

Regiel-Futyra A, Kus-Liśkiewicz M, Sebastian V, Irusta S, Arruebo M, Stochel G, Kyzioł A - ACS Appl Mater Interfaces (2015)

Bottom Line: The resulting nanocomposites did not show any cytotoxicity against mammalian somatic and tumoral cells.They produced a disruptive effect on the bacteria wall while their internalization was hindered on the eukaryotic cells.This selectivity and safety make them potentially applicable as antimicrobial coatings in the biomedical field.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Chemistry, Jagiellonian University , Ingardena 3, 30-060 Kraków, Poland.

ABSTRACT
This work describes the synthesis and characterization of noncytotoxic nanocomposites either colloidal or as films exhibiting high antibacterial activity. The biocompatible and biodegradable polymer chitosan was used as reducing and stabilizing agent for the synthesis of gold nanoparticles embedded in it. Herein, for the first time, three different chitosan grades varying in the average molecular weight and deacetylation degree (DD) were used with an optimized gold precursor concentration. Several factors were analyzed in order to obtain antimicrobial but not cytotoxic nanocomposite materials. Films based on chitosan with medium molecular weight and the highest DD exhibited the highest antibacterial activity against biofilm forming strains of Staphylococcus aureus and Pseudomonas aeruginosa. The resulting nanocomposites did not show any cytotoxicity against mammalian somatic and tumoral cells. They produced a disruptive effect on the bacteria wall while their internalization was hindered on the eukaryotic cells. This selectivity and safety make them potentially applicable as antimicrobial coatings in the biomedical field.

Show MeSH

Related in: MedlinePlus

(A) Chitosan based gold nanoparticle colloidsafter synthesis (1,2 and 5 mM gold precursor initial concentration, respectively); (B)photographs of chitosan–gold films with different AuNP loadings(1, 2 and 5 mM, respectively).
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4326049&req=5

fig1: (A) Chitosan based gold nanoparticle colloidsafter synthesis (1,2 and 5 mM gold precursor initial concentration, respectively); (B)photographs of chitosan–gold films with different AuNP loadings(1, 2 and 5 mM, respectively).

Mentions: It has been previously demonstrated that an environmentally-friendlysynthesis can be applied for the preparation of gold nanoparticleswith chitosan acting as both reducing and stabilizing agent.56 Following this approach, we prepared in situcolloidal gold nanoparticles by direct tetrachloroauric acid reductionin chitosan solutions at 60 °C. To study the influence of thepolymer properties on the resulting AuNP characteristics, for thefirst time, three different chitosan forms were used varying theiraverage molecular weight and deacetylation degree. A dependence ofthe gold concentration with the color of the resulting dispersionsafter nanoparticle formation was observed (Figure 1A). Clearly, the higher gold precursor initial concentration,the more intense the color of the subsequent colloid. The electrostaticattraction between positively charged amino groups of the polymericchains and the negatively charged gold ions (AuCl4–) results in gold reduction and NP stabilization.57 Colloidal AuNP suspensions were afterward usedfor the fabrication of films. Figure 1B showsthe resulting CS-AuNP films.


Development of noncytotoxic chitosan-gold nanocomposites as efficient antibacterial materials.

Regiel-Futyra A, Kus-Liśkiewicz M, Sebastian V, Irusta S, Arruebo M, Stochel G, Kyzioł A - ACS Appl Mater Interfaces (2015)

(A) Chitosan based gold nanoparticle colloidsafter synthesis (1,2 and 5 mM gold precursor initial concentration, respectively); (B)photographs of chitosan–gold films with different AuNP loadings(1, 2 and 5 mM, respectively).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4326049&req=5

fig1: (A) Chitosan based gold nanoparticle colloidsafter synthesis (1,2 and 5 mM gold precursor initial concentration, respectively); (B)photographs of chitosan–gold films with different AuNP loadings(1, 2 and 5 mM, respectively).
Mentions: It has been previously demonstrated that an environmentally-friendlysynthesis can be applied for the preparation of gold nanoparticleswith chitosan acting as both reducing and stabilizing agent.56 Following this approach, we prepared in situcolloidal gold nanoparticles by direct tetrachloroauric acid reductionin chitosan solutions at 60 °C. To study the influence of thepolymer properties on the resulting AuNP characteristics, for thefirst time, three different chitosan forms were used varying theiraverage molecular weight and deacetylation degree. A dependence ofthe gold concentration with the color of the resulting dispersionsafter nanoparticle formation was observed (Figure 1A). Clearly, the higher gold precursor initial concentration,the more intense the color of the subsequent colloid. The electrostaticattraction between positively charged amino groups of the polymericchains and the negatively charged gold ions (AuCl4–) results in gold reduction and NP stabilization.57 Colloidal AuNP suspensions were afterward usedfor the fabrication of films. Figure 1B showsthe resulting CS-AuNP films.

Bottom Line: The resulting nanocomposites did not show any cytotoxicity against mammalian somatic and tumoral cells.They produced a disruptive effect on the bacteria wall while their internalization was hindered on the eukaryotic cells.This selectivity and safety make them potentially applicable as antimicrobial coatings in the biomedical field.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Chemistry, Jagiellonian University , Ingardena 3, 30-060 Kraków, Poland.

ABSTRACT
This work describes the synthesis and characterization of noncytotoxic nanocomposites either colloidal or as films exhibiting high antibacterial activity. The biocompatible and biodegradable polymer chitosan was used as reducing and stabilizing agent for the synthesis of gold nanoparticles embedded in it. Herein, for the first time, three different chitosan grades varying in the average molecular weight and deacetylation degree (DD) were used with an optimized gold precursor concentration. Several factors were analyzed in order to obtain antimicrobial but not cytotoxic nanocomposite materials. Films based on chitosan with medium molecular weight and the highest DD exhibited the highest antibacterial activity against biofilm forming strains of Staphylococcus aureus and Pseudomonas aeruginosa. The resulting nanocomposites did not show any cytotoxicity against mammalian somatic and tumoral cells. They produced a disruptive effect on the bacteria wall while their internalization was hindered on the eukaryotic cells. This selectivity and safety make them potentially applicable as antimicrobial coatings in the biomedical field.

Show MeSH
Related in: MedlinePlus