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The Resin from Protium heptaphyllum Prevents High-Fat Diet-Induced Obesity in Mice: Scientific Evidence and Potential Mechanisms.

Carvalho KM, Marinho Filho JD, de Melo TS, Araújo AJ, Quetz Jda S, da Cunha Mdo P, de Melo KM, da Silva AA, Tomé AR, Havt A, Fonseca SG, Brito GA, Chaves MH, Rao VS, Santos FA - Evid Based Complement Alternat Med (2015)

Bottom Line: Additionally, RPH treatment, while significantly elevating the plasma level of ghrelin hormone, decreased the levels of insulin, leptin, and resistin.Besides, HFD-induced increases in plasma levels of proinflammatory mediators TNF-α, IL-6, and MCP-1 were significantly lowered by RPH.These findings suggest that the antiobese potential of RPH is largely due to its modulatory effects on various hormonal and enzymatic secretions related to fat and carbohydrate metabolism and to the regulation of obesity-associated inflammation.

View Article: PubMed Central - PubMed

Affiliation: Post-Graduate Programme in Medical Sciences, Faculty of Medicine, Federal University of Ceará, 60430-140 Fortaleza, CE, Brazil ; Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceará, 60430-270 Fortaleza, CE, Brazil.

ABSTRACT
Herbal compounds rich in triterpenes are well known to regulate glucose and lipid metabolism and to have beneficial effects on metabolic disorders. The present study investigated the antiobesity properties of resin from Protium heptaphyllum (RPH) and the possible mechanisms in mice fed a high-fat diet (HFD) for 15 weeks. Mice treated with RPH showed decreases in body weight, net energy intake, abdominal fat accumulation, plasma glucose, amylase, lipase, triglycerides, and total cholesterol relative to their respective controls, which were RPH unfed. Additionally, RPH treatment, while significantly elevating the plasma level of ghrelin hormone, decreased the levels of insulin, leptin, and resistin. Besides, HFD-induced increases in plasma levels of proinflammatory mediators TNF-α, IL-6, and MCP-1 were significantly lowered by RPH. Furthermore, in vitro studies revealed that RPH could significantly inhibit the lipid accumulation in 3T3-L1 adipocytes (measured by Oil-Red O staining) at concentrations up to 50 μg/mL. These findings suggest that the antiobese potential of RPH is largely due to its modulatory effects on various hormonal and enzymatic secretions related to fat and carbohydrate metabolism and to the regulation of obesity-associated inflammation.

No MeSH data available.


Related in: MedlinePlus

Effect of RPH on the expression of proteins PPARγ, C/EBPβ, and C/EBPα and in 3T3-L1 cells. β-actin protein was used as control. Postconfluent 3T3-L1 cells were differentiated in the absence or presence of RPH 12.5, 25, and 50 μg/mL for 11 days. Proteins expression was evaluated by Western blot analysis (a). Bar graphs represent densitometric results of bands PPARγ (b), C/EBPβ (c), and C/EBPα (d). UC indicates undifferentiated cell control and DC indicates differentiated cell control. While RPH significantly attenuated the expression levels of C/EBPβ at all concentration levels, statistically significant suppression was noticed for PPARγ at 25 and 50 μg/mL and for C/EBPα at 50 μg/mL only. Values are shown as mean ± SEM of three independent experiments. *P < 0.05 compared to untreated cell control (day 0).
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fig7: Effect of RPH on the expression of proteins PPARγ, C/EBPβ, and C/EBPα and in 3T3-L1 cells. β-actin protein was used as control. Postconfluent 3T3-L1 cells were differentiated in the absence or presence of RPH 12.5, 25, and 50 μg/mL for 11 days. Proteins expression was evaluated by Western blot analysis (a). Bar graphs represent densitometric results of bands PPARγ (b), C/EBPβ (c), and C/EBPα (d). UC indicates undifferentiated cell control and DC indicates differentiated cell control. While RPH significantly attenuated the expression levels of C/EBPβ at all concentration levels, statistically significant suppression was noticed for PPARγ at 25 and 50 μg/mL and for C/EBPα at 50 μg/mL only. Values are shown as mean ± SEM of three independent experiments. *P < 0.05 compared to untreated cell control (day 0).

Mentions: To investigate whether RPH (12.5, 25, and 50 μg/mL) suppresses adipogenesis through a PPARγ pathway, protein expressions of PPARγ, C/EBPα, and C/EBPβ were evaluated by Western blot analysis. The expressions of PPARγ, C/EBPα, and C/EBPβ were inhibited by RPH (Figures 7(a) and 7(b)). We also demonstrated that treatment with RPH resulted in suppression of PPARγ, C/EBPα, and C/EBPβ. While RPH significantly attenuated the expression levels of C/EBPβ at all concentration levels, statistically significant suppression was noticed for PPARγ at 25 and 50 μg/mL and for C/EBPα at 50 μg/mL only (Figures 7(c) and 7(d)). PPARγ and C/EBPα protein levels were reduced up to 65% by treatment with 50 μg/mL of RPH, while C/EBPβ protein levels were reduced up to 50% by treatment with 50 μg/mL of resin.


The Resin from Protium heptaphyllum Prevents High-Fat Diet-Induced Obesity in Mice: Scientific Evidence and Potential Mechanisms.

Carvalho KM, Marinho Filho JD, de Melo TS, Araújo AJ, Quetz Jda S, da Cunha Mdo P, de Melo KM, da Silva AA, Tomé AR, Havt A, Fonseca SG, Brito GA, Chaves MH, Rao VS, Santos FA - Evid Based Complement Alternat Med (2015)

Effect of RPH on the expression of proteins PPARγ, C/EBPβ, and C/EBPα and in 3T3-L1 cells. β-actin protein was used as control. Postconfluent 3T3-L1 cells were differentiated in the absence or presence of RPH 12.5, 25, and 50 μg/mL for 11 days. Proteins expression was evaluated by Western blot analysis (a). Bar graphs represent densitometric results of bands PPARγ (b), C/EBPβ (c), and C/EBPα (d). UC indicates undifferentiated cell control and DC indicates differentiated cell control. While RPH significantly attenuated the expression levels of C/EBPβ at all concentration levels, statistically significant suppression was noticed for PPARγ at 25 and 50 μg/mL and for C/EBPα at 50 μg/mL only. Values are shown as mean ± SEM of three independent experiments. *P < 0.05 compared to untreated cell control (day 0).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4325975&req=5

fig7: Effect of RPH on the expression of proteins PPARγ, C/EBPβ, and C/EBPα and in 3T3-L1 cells. β-actin protein was used as control. Postconfluent 3T3-L1 cells were differentiated in the absence or presence of RPH 12.5, 25, and 50 μg/mL for 11 days. Proteins expression was evaluated by Western blot analysis (a). Bar graphs represent densitometric results of bands PPARγ (b), C/EBPβ (c), and C/EBPα (d). UC indicates undifferentiated cell control and DC indicates differentiated cell control. While RPH significantly attenuated the expression levels of C/EBPβ at all concentration levels, statistically significant suppression was noticed for PPARγ at 25 and 50 μg/mL and for C/EBPα at 50 μg/mL only. Values are shown as mean ± SEM of three independent experiments. *P < 0.05 compared to untreated cell control (day 0).
Mentions: To investigate whether RPH (12.5, 25, and 50 μg/mL) suppresses adipogenesis through a PPARγ pathway, protein expressions of PPARγ, C/EBPα, and C/EBPβ were evaluated by Western blot analysis. The expressions of PPARγ, C/EBPα, and C/EBPβ were inhibited by RPH (Figures 7(a) and 7(b)). We also demonstrated that treatment with RPH resulted in suppression of PPARγ, C/EBPα, and C/EBPβ. While RPH significantly attenuated the expression levels of C/EBPβ at all concentration levels, statistically significant suppression was noticed for PPARγ at 25 and 50 μg/mL and for C/EBPα at 50 μg/mL only (Figures 7(c) and 7(d)). PPARγ and C/EBPα protein levels were reduced up to 65% by treatment with 50 μg/mL of RPH, while C/EBPβ protein levels were reduced up to 50% by treatment with 50 μg/mL of resin.

Bottom Line: Additionally, RPH treatment, while significantly elevating the plasma level of ghrelin hormone, decreased the levels of insulin, leptin, and resistin.Besides, HFD-induced increases in plasma levels of proinflammatory mediators TNF-α, IL-6, and MCP-1 were significantly lowered by RPH.These findings suggest that the antiobese potential of RPH is largely due to its modulatory effects on various hormonal and enzymatic secretions related to fat and carbohydrate metabolism and to the regulation of obesity-associated inflammation.

View Article: PubMed Central - PubMed

Affiliation: Post-Graduate Programme in Medical Sciences, Faculty of Medicine, Federal University of Ceará, 60430-140 Fortaleza, CE, Brazil ; Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceará, 60430-270 Fortaleza, CE, Brazil.

ABSTRACT
Herbal compounds rich in triterpenes are well known to regulate glucose and lipid metabolism and to have beneficial effects on metabolic disorders. The present study investigated the antiobesity properties of resin from Protium heptaphyllum (RPH) and the possible mechanisms in mice fed a high-fat diet (HFD) for 15 weeks. Mice treated with RPH showed decreases in body weight, net energy intake, abdominal fat accumulation, plasma glucose, amylase, lipase, triglycerides, and total cholesterol relative to their respective controls, which were RPH unfed. Additionally, RPH treatment, while significantly elevating the plasma level of ghrelin hormone, decreased the levels of insulin, leptin, and resistin. Besides, HFD-induced increases in plasma levels of proinflammatory mediators TNF-α, IL-6, and MCP-1 were significantly lowered by RPH. Furthermore, in vitro studies revealed that RPH could significantly inhibit the lipid accumulation in 3T3-L1 adipocytes (measured by Oil-Red O staining) at concentrations up to 50 μg/mL. These findings suggest that the antiobese potential of RPH is largely due to its modulatory effects on various hormonal and enzymatic secretions related to fat and carbohydrate metabolism and to the regulation of obesity-associated inflammation.

No MeSH data available.


Related in: MedlinePlus