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The Resin from Protium heptaphyllum Prevents High-Fat Diet-Induced Obesity in Mice: Scientific Evidence and Potential Mechanisms.

Carvalho KM, Marinho Filho JD, de Melo TS, Araújo AJ, Quetz Jda S, da Cunha Mdo P, de Melo KM, da Silva AA, Tomé AR, Havt A, Fonseca SG, Brito GA, Chaves MH, Rao VS, Santos FA - Evid Based Complement Alternat Med (2015)

Bottom Line: Additionally, RPH treatment, while significantly elevating the plasma level of ghrelin hormone, decreased the levels of insulin, leptin, and resistin.Besides, HFD-induced increases in plasma levels of proinflammatory mediators TNF-α, IL-6, and MCP-1 were significantly lowered by RPH.These findings suggest that the antiobese potential of RPH is largely due to its modulatory effects on various hormonal and enzymatic secretions related to fat and carbohydrate metabolism and to the regulation of obesity-associated inflammation.

View Article: PubMed Central - PubMed

Affiliation: Post-Graduate Programme in Medical Sciences, Faculty of Medicine, Federal University of Ceará, 60430-140 Fortaleza, CE, Brazil ; Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceará, 60430-270 Fortaleza, CE, Brazil.

ABSTRACT
Herbal compounds rich in triterpenes are well known to regulate glucose and lipid metabolism and to have beneficial effects on metabolic disorders. The present study investigated the antiobesity properties of resin from Protium heptaphyllum (RPH) and the possible mechanisms in mice fed a high-fat diet (HFD) for 15 weeks. Mice treated with RPH showed decreases in body weight, net energy intake, abdominal fat accumulation, plasma glucose, amylase, lipase, triglycerides, and total cholesterol relative to their respective controls, which were RPH unfed. Additionally, RPH treatment, while significantly elevating the plasma level of ghrelin hormone, decreased the levels of insulin, leptin, and resistin. Besides, HFD-induced increases in plasma levels of proinflammatory mediators TNF-α, IL-6, and MCP-1 were significantly lowered by RPH. Furthermore, in vitro studies revealed that RPH could significantly inhibit the lipid accumulation in 3T3-L1 adipocytes (measured by Oil-Red O staining) at concentrations up to 50 μg/mL. These findings suggest that the antiobese potential of RPH is largely due to its modulatory effects on various hormonal and enzymatic secretions related to fat and carbohydrate metabolism and to the regulation of obesity-associated inflammation.

No MeSH data available.


Related in: MedlinePlus

Effect of RPH on lipid accumulation in 3T3-L1 cells. Oil-Red O staining representing the effect of induction of differentiation (a) and the effect of RPH 6.25, 12.5, 25, and 50 μg/mL (b) on lipid accumulation in 3T3-L1 cells (400x magnification). The staining intensity of Oil-Red O was measured at 510 nm wavelength and quantified ((c) and (d)). Values are expressed as mean ± SEM of three independent experiments. *P < 0.05 compared with untreated cell control (day 0).
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fig6: Effect of RPH on lipid accumulation in 3T3-L1 cells. Oil-Red O staining representing the effect of induction of differentiation (a) and the effect of RPH 6.25, 12.5, 25, and 50 μg/mL (b) on lipid accumulation in 3T3-L1 cells (400x magnification). The staining intensity of Oil-Red O was measured at 510 nm wavelength and quantified ((c) and (d)). Values are expressed as mean ± SEM of three independent experiments. *P < 0.05 compared with untreated cell control (day 0).

Mentions: The lipid droplets appeared at around day 5. Images of representative cells with lipid droplets on days 7, 9, 11, and 13 were shown in Figure 6(a) and the quantified staining intensity of Oil-Red O in Figure 6(c). Oil-Red O staining revealed a significant reduction in lipid accumulation with RPH (12.5, 25, and 50 μg/mL) in 3T3-L1 adipocytes (Figures 6(b) and 6(d)).


The Resin from Protium heptaphyllum Prevents High-Fat Diet-Induced Obesity in Mice: Scientific Evidence and Potential Mechanisms.

Carvalho KM, Marinho Filho JD, de Melo TS, Araújo AJ, Quetz Jda S, da Cunha Mdo P, de Melo KM, da Silva AA, Tomé AR, Havt A, Fonseca SG, Brito GA, Chaves MH, Rao VS, Santos FA - Evid Based Complement Alternat Med (2015)

Effect of RPH on lipid accumulation in 3T3-L1 cells. Oil-Red O staining representing the effect of induction of differentiation (a) and the effect of RPH 6.25, 12.5, 25, and 50 μg/mL (b) on lipid accumulation in 3T3-L1 cells (400x magnification). The staining intensity of Oil-Red O was measured at 510 nm wavelength and quantified ((c) and (d)). Values are expressed as mean ± SEM of three independent experiments. *P < 0.05 compared with untreated cell control (day 0).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4325975&req=5

fig6: Effect of RPH on lipid accumulation in 3T3-L1 cells. Oil-Red O staining representing the effect of induction of differentiation (a) and the effect of RPH 6.25, 12.5, 25, and 50 μg/mL (b) on lipid accumulation in 3T3-L1 cells (400x magnification). The staining intensity of Oil-Red O was measured at 510 nm wavelength and quantified ((c) and (d)). Values are expressed as mean ± SEM of three independent experiments. *P < 0.05 compared with untreated cell control (day 0).
Mentions: The lipid droplets appeared at around day 5. Images of representative cells with lipid droplets on days 7, 9, 11, and 13 were shown in Figure 6(a) and the quantified staining intensity of Oil-Red O in Figure 6(c). Oil-Red O staining revealed a significant reduction in lipid accumulation with RPH (12.5, 25, and 50 μg/mL) in 3T3-L1 adipocytes (Figures 6(b) and 6(d)).

Bottom Line: Additionally, RPH treatment, while significantly elevating the plasma level of ghrelin hormone, decreased the levels of insulin, leptin, and resistin.Besides, HFD-induced increases in plasma levels of proinflammatory mediators TNF-α, IL-6, and MCP-1 were significantly lowered by RPH.These findings suggest that the antiobese potential of RPH is largely due to its modulatory effects on various hormonal and enzymatic secretions related to fat and carbohydrate metabolism and to the regulation of obesity-associated inflammation.

View Article: PubMed Central - PubMed

Affiliation: Post-Graduate Programme in Medical Sciences, Faculty of Medicine, Federal University of Ceará, 60430-140 Fortaleza, CE, Brazil ; Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceará, 60430-270 Fortaleza, CE, Brazil.

ABSTRACT
Herbal compounds rich in triterpenes are well known to regulate glucose and lipid metabolism and to have beneficial effects on metabolic disorders. The present study investigated the antiobesity properties of resin from Protium heptaphyllum (RPH) and the possible mechanisms in mice fed a high-fat diet (HFD) for 15 weeks. Mice treated with RPH showed decreases in body weight, net energy intake, abdominal fat accumulation, plasma glucose, amylase, lipase, triglycerides, and total cholesterol relative to their respective controls, which were RPH unfed. Additionally, RPH treatment, while significantly elevating the plasma level of ghrelin hormone, decreased the levels of insulin, leptin, and resistin. Besides, HFD-induced increases in plasma levels of proinflammatory mediators TNF-α, IL-6, and MCP-1 were significantly lowered by RPH. Furthermore, in vitro studies revealed that RPH could significantly inhibit the lipid accumulation in 3T3-L1 adipocytes (measured by Oil-Red O staining) at concentrations up to 50 μg/mL. These findings suggest that the antiobese potential of RPH is largely due to its modulatory effects on various hormonal and enzymatic secretions related to fat and carbohydrate metabolism and to the regulation of obesity-associated inflammation.

No MeSH data available.


Related in: MedlinePlus