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The Resin from Protium heptaphyllum Prevents High-Fat Diet-Induced Obesity in Mice: Scientific Evidence and Potential Mechanisms.

Carvalho KM, Marinho Filho JD, de Melo TS, Araújo AJ, Quetz Jda S, da Cunha Mdo P, de Melo KM, da Silva AA, Tomé AR, Havt A, Fonseca SG, Brito GA, Chaves MH, Rao VS, Santos FA - Evid Based Complement Alternat Med (2015)

Bottom Line: Additionally, RPH treatment, while significantly elevating the plasma level of ghrelin hormone, decreased the levels of insulin, leptin, and resistin.Besides, HFD-induced increases in plasma levels of proinflammatory mediators TNF-α, IL-6, and MCP-1 were significantly lowered by RPH.These findings suggest that the antiobese potential of RPH is largely due to its modulatory effects on various hormonal and enzymatic secretions related to fat and carbohydrate metabolism and to the regulation of obesity-associated inflammation.

View Article: PubMed Central - PubMed

Affiliation: Post-Graduate Programme in Medical Sciences, Faculty of Medicine, Federal University of Ceará, 60430-140 Fortaleza, CE, Brazil ; Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceará, 60430-270 Fortaleza, CE, Brazil.

ABSTRACT
Herbal compounds rich in triterpenes are well known to regulate glucose and lipid metabolism and to have beneficial effects on metabolic disorders. The present study investigated the antiobesity properties of resin from Protium heptaphyllum (RPH) and the possible mechanisms in mice fed a high-fat diet (HFD) for 15 weeks. Mice treated with RPH showed decreases in body weight, net energy intake, abdominal fat accumulation, plasma glucose, amylase, lipase, triglycerides, and total cholesterol relative to their respective controls, which were RPH unfed. Additionally, RPH treatment, while significantly elevating the plasma level of ghrelin hormone, decreased the levels of insulin, leptin, and resistin. Besides, HFD-induced increases in plasma levels of proinflammatory mediators TNF-α, IL-6, and MCP-1 were significantly lowered by RPH. Furthermore, in vitro studies revealed that RPH could significantly inhibit the lipid accumulation in 3T3-L1 adipocytes (measured by Oil-Red O staining) at concentrations up to 50 μg/mL. These findings suggest that the antiobese potential of RPH is largely due to its modulatory effects on various hormonal and enzymatic secretions related to fat and carbohydrate metabolism and to the regulation of obesity-associated inflammation.

No MeSH data available.


Related in: MedlinePlus

Histology of liver tissue of mice fed the experimental diets for 15 weeks. Histology of liver tissue of mice fed the experimental diets for 15 weeks. Representative microphotographs of mouse liver fed (a) a normal diet showing normal architecture and hepatocytes; (b) high-fat diet showing focal parenchymatous inflammatory infiltrate, isolated hepatocyte necrosis, and mild steatosis; (c) high-fat diet + RPH 20 mg/kg; and (d) high-fat diet + sibutramine that show well-formed nucleated hepatocytes, slight dilated sinusoids, minimal inflammatory lymphocyte infiltration, and absence of lipid droplets (H&E, ×100).
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fig5: Histology of liver tissue of mice fed the experimental diets for 15 weeks. Histology of liver tissue of mice fed the experimental diets for 15 weeks. Representative microphotographs of mouse liver fed (a) a normal diet showing normal architecture and hepatocytes; (b) high-fat diet showing focal parenchymatous inflammatory infiltrate, isolated hepatocyte necrosis, and mild steatosis; (c) high-fat diet + RPH 20 mg/kg; and (d) high-fat diet + sibutramine that show well-formed nucleated hepatocytes, slight dilated sinusoids, minimal inflammatory lymphocyte infiltration, and absence of lipid droplets (H&E, ×100).

Mentions: The HFD resulted in mild, focal, and parenchymatous inflammatory infiltrate, isolated hepatocyte necrosis, mild microgoticular steatosis without peculiar disposition, and the presence of lipid droplets. RPH 20 mg/kg and SIB treatments showed well-formed nucleated hepatocyte, slight dilatation of sinusoids, slight inflammatory lymphocyte infiltration, absence of fibrous tissue, and no accumulation of lipid droplets (Figure 5).


The Resin from Protium heptaphyllum Prevents High-Fat Diet-Induced Obesity in Mice: Scientific Evidence and Potential Mechanisms.

Carvalho KM, Marinho Filho JD, de Melo TS, Araújo AJ, Quetz Jda S, da Cunha Mdo P, de Melo KM, da Silva AA, Tomé AR, Havt A, Fonseca SG, Brito GA, Chaves MH, Rao VS, Santos FA - Evid Based Complement Alternat Med (2015)

Histology of liver tissue of mice fed the experimental diets for 15 weeks. Histology of liver tissue of mice fed the experimental diets for 15 weeks. Representative microphotographs of mouse liver fed (a) a normal diet showing normal architecture and hepatocytes; (b) high-fat diet showing focal parenchymatous inflammatory infiltrate, isolated hepatocyte necrosis, and mild steatosis; (c) high-fat diet + RPH 20 mg/kg; and (d) high-fat diet + sibutramine that show well-formed nucleated hepatocytes, slight dilated sinusoids, minimal inflammatory lymphocyte infiltration, and absence of lipid droplets (H&E, ×100).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4325975&req=5

fig5: Histology of liver tissue of mice fed the experimental diets for 15 weeks. Histology of liver tissue of mice fed the experimental diets for 15 weeks. Representative microphotographs of mouse liver fed (a) a normal diet showing normal architecture and hepatocytes; (b) high-fat diet showing focal parenchymatous inflammatory infiltrate, isolated hepatocyte necrosis, and mild steatosis; (c) high-fat diet + RPH 20 mg/kg; and (d) high-fat diet + sibutramine that show well-formed nucleated hepatocytes, slight dilated sinusoids, minimal inflammatory lymphocyte infiltration, and absence of lipid droplets (H&E, ×100).
Mentions: The HFD resulted in mild, focal, and parenchymatous inflammatory infiltrate, isolated hepatocyte necrosis, mild microgoticular steatosis without peculiar disposition, and the presence of lipid droplets. RPH 20 mg/kg and SIB treatments showed well-formed nucleated hepatocyte, slight dilatation of sinusoids, slight inflammatory lymphocyte infiltration, absence of fibrous tissue, and no accumulation of lipid droplets (Figure 5).

Bottom Line: Additionally, RPH treatment, while significantly elevating the plasma level of ghrelin hormone, decreased the levels of insulin, leptin, and resistin.Besides, HFD-induced increases in plasma levels of proinflammatory mediators TNF-α, IL-6, and MCP-1 were significantly lowered by RPH.These findings suggest that the antiobese potential of RPH is largely due to its modulatory effects on various hormonal and enzymatic secretions related to fat and carbohydrate metabolism and to the regulation of obesity-associated inflammation.

View Article: PubMed Central - PubMed

Affiliation: Post-Graduate Programme in Medical Sciences, Faculty of Medicine, Federal University of Ceará, 60430-140 Fortaleza, CE, Brazil ; Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceará, 60430-270 Fortaleza, CE, Brazil.

ABSTRACT
Herbal compounds rich in triterpenes are well known to regulate glucose and lipid metabolism and to have beneficial effects on metabolic disorders. The present study investigated the antiobesity properties of resin from Protium heptaphyllum (RPH) and the possible mechanisms in mice fed a high-fat diet (HFD) for 15 weeks. Mice treated with RPH showed decreases in body weight, net energy intake, abdominal fat accumulation, plasma glucose, amylase, lipase, triglycerides, and total cholesterol relative to their respective controls, which were RPH unfed. Additionally, RPH treatment, while significantly elevating the plasma level of ghrelin hormone, decreased the levels of insulin, leptin, and resistin. Besides, HFD-induced increases in plasma levels of proinflammatory mediators TNF-α, IL-6, and MCP-1 were significantly lowered by RPH. Furthermore, in vitro studies revealed that RPH could significantly inhibit the lipid accumulation in 3T3-L1 adipocytes (measured by Oil-Red O staining) at concentrations up to 50 μg/mL. These findings suggest that the antiobese potential of RPH is largely due to its modulatory effects on various hormonal and enzymatic secretions related to fat and carbohydrate metabolism and to the regulation of obesity-associated inflammation.

No MeSH data available.


Related in: MedlinePlus