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The Resin from Protium heptaphyllum Prevents High-Fat Diet-Induced Obesity in Mice: Scientific Evidence and Potential Mechanisms.

Carvalho KM, Marinho Filho JD, de Melo TS, Araújo AJ, Quetz Jda S, da Cunha Mdo P, de Melo KM, da Silva AA, Tomé AR, Havt A, Fonseca SG, Brito GA, Chaves MH, Rao VS, Santos FA - Evid Based Complement Alternat Med (2015)

Bottom Line: Additionally, RPH treatment, while significantly elevating the plasma level of ghrelin hormone, decreased the levels of insulin, leptin, and resistin.Besides, HFD-induced increases in plasma levels of proinflammatory mediators TNF-α, IL-6, and MCP-1 were significantly lowered by RPH.These findings suggest that the antiobese potential of RPH is largely due to its modulatory effects on various hormonal and enzymatic secretions related to fat and carbohydrate metabolism and to the regulation of obesity-associated inflammation.

View Article: PubMed Central - PubMed

Affiliation: Post-Graduate Programme in Medical Sciences, Faculty of Medicine, Federal University of Ceará, 60430-140 Fortaleza, CE, Brazil ; Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceará, 60430-270 Fortaleza, CE, Brazil.

ABSTRACT
Herbal compounds rich in triterpenes are well known to regulate glucose and lipid metabolism and to have beneficial effects on metabolic disorders. The present study investigated the antiobesity properties of resin from Protium heptaphyllum (RPH) and the possible mechanisms in mice fed a high-fat diet (HFD) for 15 weeks. Mice treated with RPH showed decreases in body weight, net energy intake, abdominal fat accumulation, plasma glucose, amylase, lipase, triglycerides, and total cholesterol relative to their respective controls, which were RPH unfed. Additionally, RPH treatment, while significantly elevating the plasma level of ghrelin hormone, decreased the levels of insulin, leptin, and resistin. Besides, HFD-induced increases in plasma levels of proinflammatory mediators TNF-α, IL-6, and MCP-1 were significantly lowered by RPH. Furthermore, in vitro studies revealed that RPH could significantly inhibit the lipid accumulation in 3T3-L1 adipocytes (measured by Oil-Red O staining) at concentrations up to 50 μg/mL. These findings suggest that the antiobese potential of RPH is largely due to its modulatory effects on various hormonal and enzymatic secretions related to fat and carbohydrate metabolism and to the regulation of obesity-associated inflammation.

No MeSH data available.


Related in: MedlinePlus

Effects of resin of Protium heptaphyllum (RPH) and sibutramine (SIB) treatments on TNF-α (a), IL-6 (b), and MCP-1 (c) levels in mice fed experimental diets for 15 weeks. ND: normal diet; HFD: high-fat diet. Each value is the mean ± SEM (n = 6–8). aP < 0.05 versus ND group. bP < 0.05 versus HFD group.
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Related In: Results  -  Collection


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fig2: Effects of resin of Protium heptaphyllum (RPH) and sibutramine (SIB) treatments on TNF-α (a), IL-6 (b), and MCP-1 (c) levels in mice fed experimental diets for 15 weeks. ND: normal diet; HFD: high-fat diet. Each value is the mean ± SEM (n = 6–8). aP < 0.05 versus ND group. bP < 0.05 versus HFD group.

Mentions: Mice on HFD had a lower level of ghrelin but a higher leptin and resistin levels compared to the ND group. Unlike the RPH treatment, which showed a further increase in the plasma level of ghrelin, SIB treatment had no significant influence on the HFD-induced change in ghrelin (Figure 1(a)). Plasma levels of leptin were significantly less in groups of mice treated with RPH or SIB compared to the HFD control (Figure 1(b)). While SIB treatment demonstrated no significant influence on the HFD-induced increase in the resistin level, RPH 20 mg/kg treatment significantly lowered the level of plasma resistin (Figure 1(c)). Mice on HFD demonstrated higher levels of TNF-α, IL-6 and MCP-1 compared to the ND group. While SIB treatment showed no significant influence on HFD-induced changes in the levels of TNF-α, RPH 20 mg/kg treatment caused a significant decrease in TNF-α level (Figure 2(a)). RPH and SIB treatments, however, reduced the IL-6 and MCP-1 levels compared to mice on HFD (Figures 2(b) and 2(c)).


The Resin from Protium heptaphyllum Prevents High-Fat Diet-Induced Obesity in Mice: Scientific Evidence and Potential Mechanisms.

Carvalho KM, Marinho Filho JD, de Melo TS, Araújo AJ, Quetz Jda S, da Cunha Mdo P, de Melo KM, da Silva AA, Tomé AR, Havt A, Fonseca SG, Brito GA, Chaves MH, Rao VS, Santos FA - Evid Based Complement Alternat Med (2015)

Effects of resin of Protium heptaphyllum (RPH) and sibutramine (SIB) treatments on TNF-α (a), IL-6 (b), and MCP-1 (c) levels in mice fed experimental diets for 15 weeks. ND: normal diet; HFD: high-fat diet. Each value is the mean ± SEM (n = 6–8). aP < 0.05 versus ND group. bP < 0.05 versus HFD group.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4325975&req=5

fig2: Effects of resin of Protium heptaphyllum (RPH) and sibutramine (SIB) treatments on TNF-α (a), IL-6 (b), and MCP-1 (c) levels in mice fed experimental diets for 15 weeks. ND: normal diet; HFD: high-fat diet. Each value is the mean ± SEM (n = 6–8). aP < 0.05 versus ND group. bP < 0.05 versus HFD group.
Mentions: Mice on HFD had a lower level of ghrelin but a higher leptin and resistin levels compared to the ND group. Unlike the RPH treatment, which showed a further increase in the plasma level of ghrelin, SIB treatment had no significant influence on the HFD-induced change in ghrelin (Figure 1(a)). Plasma levels of leptin were significantly less in groups of mice treated with RPH or SIB compared to the HFD control (Figure 1(b)). While SIB treatment demonstrated no significant influence on the HFD-induced increase in the resistin level, RPH 20 mg/kg treatment significantly lowered the level of plasma resistin (Figure 1(c)). Mice on HFD demonstrated higher levels of TNF-α, IL-6 and MCP-1 compared to the ND group. While SIB treatment showed no significant influence on HFD-induced changes in the levels of TNF-α, RPH 20 mg/kg treatment caused a significant decrease in TNF-α level (Figure 2(a)). RPH and SIB treatments, however, reduced the IL-6 and MCP-1 levels compared to mice on HFD (Figures 2(b) and 2(c)).

Bottom Line: Additionally, RPH treatment, while significantly elevating the plasma level of ghrelin hormone, decreased the levels of insulin, leptin, and resistin.Besides, HFD-induced increases in plasma levels of proinflammatory mediators TNF-α, IL-6, and MCP-1 were significantly lowered by RPH.These findings suggest that the antiobese potential of RPH is largely due to its modulatory effects on various hormonal and enzymatic secretions related to fat and carbohydrate metabolism and to the regulation of obesity-associated inflammation.

View Article: PubMed Central - PubMed

Affiliation: Post-Graduate Programme in Medical Sciences, Faculty of Medicine, Federal University of Ceará, 60430-140 Fortaleza, CE, Brazil ; Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceará, 60430-270 Fortaleza, CE, Brazil.

ABSTRACT
Herbal compounds rich in triterpenes are well known to regulate glucose and lipid metabolism and to have beneficial effects on metabolic disorders. The present study investigated the antiobesity properties of resin from Protium heptaphyllum (RPH) and the possible mechanisms in mice fed a high-fat diet (HFD) for 15 weeks. Mice treated with RPH showed decreases in body weight, net energy intake, abdominal fat accumulation, plasma glucose, amylase, lipase, triglycerides, and total cholesterol relative to their respective controls, which were RPH unfed. Additionally, RPH treatment, while significantly elevating the plasma level of ghrelin hormone, decreased the levels of insulin, leptin, and resistin. Besides, HFD-induced increases in plasma levels of proinflammatory mediators TNF-α, IL-6, and MCP-1 were significantly lowered by RPH. Furthermore, in vitro studies revealed that RPH could significantly inhibit the lipid accumulation in 3T3-L1 adipocytes (measured by Oil-Red O staining) at concentrations up to 50 μg/mL. These findings suggest that the antiobese potential of RPH is largely due to its modulatory effects on various hormonal and enzymatic secretions related to fat and carbohydrate metabolism and to the regulation of obesity-associated inflammation.

No MeSH data available.


Related in: MedlinePlus