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High in vitro antibacterial activity of Pac-525 against Porphyromonas gingivalis biofilms cultured on titanium.

Li JY, Wang XJ, Wang LN, Ying XX, Ren X, Liu HY, Xu L, Ma GW - Biomed Res Int (2015)

Bottom Line: The minimum inhibitory concentration and minimum bactericidal concentration values of Pac-525 were 0.0625 mg/mL and 0.125 mg/mL, respectively, for P. gingivalis and 0.0078 mg/mL and 0.0156 mg/mL, respectively, for F. nucleatum.Using CLSM, we confirmed that compared to 0.1% chlorhexidine, 0.5 mg/mL of Pac-525 caused a significant decrease in biofilm thickness and a decline in the percentage of live bacteria.These data indicate that Pac-525 has unique properties that might make it suitable for the inhibition the growth of pathogenic bacteria around dental implants.

View Article: PubMed Central - PubMed

Affiliation: Department of Prosthodontics, School of Stomatology, Dalian Medical University, Dalian 116044, China.

ABSTRACT
In order to investigate the potential of short antimicrobial peptides (AMPs) as alternative antibacterial agents during the treatment of peri-implantitis, the cytotoxic activity of three short AMPs, that is, Pac-525, KSL-W, and KSL, was determined using the MTT assay. The antimicrobial activity of these AMPs, ranging in concentration from 0.0039 mg/mL to 0.5 mg/mL, against the predominant planktonic pathogens, including Streptococcus sanguis, Fusobacterium nucleatum, and Porphyromonas gingivalis, involved in peri-implantitis was investigated. Furthermore, 2-day-old P. gingivalis biofilms cultured on titanium surfaces were treated with Pac-525 and subsequently observed and analysed using confocal laser scanning microscopy (CLSM). The average cell proliferation curve indicated that there was no cytotoxicity due to the three short AMPs. The minimum inhibitory concentration and minimum bactericidal concentration values of Pac-525 were 0.0625 mg/mL and 0.125 mg/mL, respectively, for P. gingivalis and 0.0078 mg/mL and 0.0156 mg/mL, respectively, for F. nucleatum. Using CLSM, we confirmed that compared to 0.1% chlorhexidine, 0.5 mg/mL of Pac-525 caused a significant decrease in biofilm thickness and a decline in the percentage of live bacteria. These data indicate that Pac-525 has unique properties that might make it suitable for the inhibition the growth of pathogenic bacteria around dental implants.

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Percentage of live bacteria in P. gingivalis biofilms (on titanium discs) treated with Pac-525. *Significantly different from untreated control. #Significantly different from CHX-treated control (P < 0.05).
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fig6: Percentage of live bacteria in P. gingivalis biofilms (on titanium discs) treated with Pac-525. *Significantly different from untreated control. #Significantly different from CHX-treated control (P < 0.05).

Mentions: P. gingivalis biofilms on the surface of titanium discs were imaged by CLSM and are shown in Figure 4. In the images, the live bacteria were stained and emitted a green fluorescence signal, while the dead one was stained and produced red fluorescence. The nontreated control biofilm emitted green fluorescence (a), and the CHX-treated biofilm emitted red (b). Compared to the controls, Pac-525 had a significant impact on the viability of biofilm cells. The biofilm showed extensive green fluorescence and scattered red fluorescence after treatment with 0.125 mg/mL Pac-525 (c), obvious red fluorescence and inconspicuous green fluorescence with 0.25 mg/mL Pac-525 (d), and almost completely red fluorescence with 0.5 mg/mL Pac-525 (e). The average thickness of P. gingivalis biofilms was 55.89 μm and 41.64 μm at Pac-525 concentrations of 0.25 mg/mL and 0.5 mg/mL, respectively, which were significantly lower than those in the nontreated control group (P < 0.05). Furthermore, the thickness of biofilms treated with 0.5 mg/mL Pac-525 was significantly lower than that in the CHX-treated control group (P < 0.05, Figure 5). Similar to the results for biofilm thickness, live bacteria percentages were 0.39 ± 0.02 and 0.30 ± 0.02 after treatment with Pac-525 concentrations of 0.25 mg/mL and 0.5 mg/mL, respectively. These values were significantly lower than those of the nontreated control group (P < 0.05), whereas the live bacteria percentage of the 0.5 mg/mL Pac-525-treated group was significantly lower than that of the CHX-treated control group (P < 0.05, Figure 6), suggesting that Pac-525, especially at a concentration of 0.5 mg/mL, had the favourable ability to decrease biofilm thickness, diffuse cell distribution, and reduce P. gingivalis viability. These results indicated that Pac-525 was not only able to kill P. gingivalis within the biofilm on the titanium but was also able to cause the mature biofilm to disintegrate.


High in vitro antibacterial activity of Pac-525 against Porphyromonas gingivalis biofilms cultured on titanium.

Li JY, Wang XJ, Wang LN, Ying XX, Ren X, Liu HY, Xu L, Ma GW - Biomed Res Int (2015)

Percentage of live bacteria in P. gingivalis biofilms (on titanium discs) treated with Pac-525. *Significantly different from untreated control. #Significantly different from CHX-treated control (P < 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

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fig6: Percentage of live bacteria in P. gingivalis biofilms (on titanium discs) treated with Pac-525. *Significantly different from untreated control. #Significantly different from CHX-treated control (P < 0.05).
Mentions: P. gingivalis biofilms on the surface of titanium discs were imaged by CLSM and are shown in Figure 4. In the images, the live bacteria were stained and emitted a green fluorescence signal, while the dead one was stained and produced red fluorescence. The nontreated control biofilm emitted green fluorescence (a), and the CHX-treated biofilm emitted red (b). Compared to the controls, Pac-525 had a significant impact on the viability of biofilm cells. The biofilm showed extensive green fluorescence and scattered red fluorescence after treatment with 0.125 mg/mL Pac-525 (c), obvious red fluorescence and inconspicuous green fluorescence with 0.25 mg/mL Pac-525 (d), and almost completely red fluorescence with 0.5 mg/mL Pac-525 (e). The average thickness of P. gingivalis biofilms was 55.89 μm and 41.64 μm at Pac-525 concentrations of 0.25 mg/mL and 0.5 mg/mL, respectively, which were significantly lower than those in the nontreated control group (P < 0.05). Furthermore, the thickness of biofilms treated with 0.5 mg/mL Pac-525 was significantly lower than that in the CHX-treated control group (P < 0.05, Figure 5). Similar to the results for biofilm thickness, live bacteria percentages were 0.39 ± 0.02 and 0.30 ± 0.02 after treatment with Pac-525 concentrations of 0.25 mg/mL and 0.5 mg/mL, respectively. These values were significantly lower than those of the nontreated control group (P < 0.05), whereas the live bacteria percentage of the 0.5 mg/mL Pac-525-treated group was significantly lower than that of the CHX-treated control group (P < 0.05, Figure 6), suggesting that Pac-525, especially at a concentration of 0.5 mg/mL, had the favourable ability to decrease biofilm thickness, diffuse cell distribution, and reduce P. gingivalis viability. These results indicated that Pac-525 was not only able to kill P. gingivalis within the biofilm on the titanium but was also able to cause the mature biofilm to disintegrate.

Bottom Line: The minimum inhibitory concentration and minimum bactericidal concentration values of Pac-525 were 0.0625 mg/mL and 0.125 mg/mL, respectively, for P. gingivalis and 0.0078 mg/mL and 0.0156 mg/mL, respectively, for F. nucleatum.Using CLSM, we confirmed that compared to 0.1% chlorhexidine, 0.5 mg/mL of Pac-525 caused a significant decrease in biofilm thickness and a decline in the percentage of live bacteria.These data indicate that Pac-525 has unique properties that might make it suitable for the inhibition the growth of pathogenic bacteria around dental implants.

View Article: PubMed Central - PubMed

Affiliation: Department of Prosthodontics, School of Stomatology, Dalian Medical University, Dalian 116044, China.

ABSTRACT
In order to investigate the potential of short antimicrobial peptides (AMPs) as alternative antibacterial agents during the treatment of peri-implantitis, the cytotoxic activity of three short AMPs, that is, Pac-525, KSL-W, and KSL, was determined using the MTT assay. The antimicrobial activity of these AMPs, ranging in concentration from 0.0039 mg/mL to 0.5 mg/mL, against the predominant planktonic pathogens, including Streptococcus sanguis, Fusobacterium nucleatum, and Porphyromonas gingivalis, involved in peri-implantitis was investigated. Furthermore, 2-day-old P. gingivalis biofilms cultured on titanium surfaces were treated with Pac-525 and subsequently observed and analysed using confocal laser scanning microscopy (CLSM). The average cell proliferation curve indicated that there was no cytotoxicity due to the three short AMPs. The minimum inhibitory concentration and minimum bactericidal concentration values of Pac-525 were 0.0625 mg/mL and 0.125 mg/mL, respectively, for P. gingivalis and 0.0078 mg/mL and 0.0156 mg/mL, respectively, for F. nucleatum. Using CLSM, we confirmed that compared to 0.1% chlorhexidine, 0.5 mg/mL of Pac-525 caused a significant decrease in biofilm thickness and a decline in the percentage of live bacteria. These data indicate that Pac-525 has unique properties that might make it suitable for the inhibition the growth of pathogenic bacteria around dental implants.

Show MeSH