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High in vitro antibacterial activity of Pac-525 against Porphyromonas gingivalis biofilms cultured on titanium.

Li JY, Wang XJ, Wang LN, Ying XX, Ren X, Liu HY, Xu L, Ma GW - Biomed Res Int (2015)

Bottom Line: The minimum inhibitory concentration and minimum bactericidal concentration values of Pac-525 were 0.0625 mg/mL and 0.125 mg/mL, respectively, for P. gingivalis and 0.0078 mg/mL and 0.0156 mg/mL, respectively, for F. nucleatum.Using CLSM, we confirmed that compared to 0.1% chlorhexidine, 0.5 mg/mL of Pac-525 caused a significant decrease in biofilm thickness and a decline in the percentage of live bacteria.These data indicate that Pac-525 has unique properties that might make it suitable for the inhibition the growth of pathogenic bacteria around dental implants.

View Article: PubMed Central - PubMed

Affiliation: Department of Prosthodontics, School of Stomatology, Dalian Medical University, Dalian 116044, China.

ABSTRACT
In order to investigate the potential of short antimicrobial peptides (AMPs) as alternative antibacterial agents during the treatment of peri-implantitis, the cytotoxic activity of three short AMPs, that is, Pac-525, KSL-W, and KSL, was determined using the MTT assay. The antimicrobial activity of these AMPs, ranging in concentration from 0.0039 mg/mL to 0.5 mg/mL, against the predominant planktonic pathogens, including Streptococcus sanguis, Fusobacterium nucleatum, and Porphyromonas gingivalis, involved in peri-implantitis was investigated. Furthermore, 2-day-old P. gingivalis biofilms cultured on titanium surfaces were treated with Pac-525 and subsequently observed and analysed using confocal laser scanning microscopy (CLSM). The average cell proliferation curve indicated that there was no cytotoxicity due to the three short AMPs. The minimum inhibitory concentration and minimum bactericidal concentration values of Pac-525 were 0.0625 mg/mL and 0.125 mg/mL, respectively, for P. gingivalis and 0.0078 mg/mL and 0.0156 mg/mL, respectively, for F. nucleatum. Using CLSM, we confirmed that compared to 0.1% chlorhexidine, 0.5 mg/mL of Pac-525 caused a significant decrease in biofilm thickness and a decline in the percentage of live bacteria. These data indicate that Pac-525 has unique properties that might make it suitable for the inhibition the growth of pathogenic bacteria around dental implants.

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Proliferation curves for MG-63 cells exposed to (a) Pac-525, (b) KSL-W, or (c) KSL antimicrobial peptide solutions at concentrations ranging from 0 to 0.5 mg/mL.
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fig2: Proliferation curves for MG-63 cells exposed to (a) Pac-525, (b) KSL-W, or (c) KSL antimicrobial peptide solutions at concentrations ranging from 0 to 0.5 mg/mL.

Mentions: Figure 2 shows the average cell proliferation curve, with the concentration of AMPs on the X-axis and OD values on the Y-axis. Compared with the untreated group, the absorbance values for each well (treated with Pac-525, KSL-W, or KSL) were similar for each concentration. There was no statistical difference between the AMP-treated group and the untreated group and no statistical difference among the eight concentrations of AMPs.


High in vitro antibacterial activity of Pac-525 against Porphyromonas gingivalis biofilms cultured on titanium.

Li JY, Wang XJ, Wang LN, Ying XX, Ren X, Liu HY, Xu L, Ma GW - Biomed Res Int (2015)

Proliferation curves for MG-63 cells exposed to (a) Pac-525, (b) KSL-W, or (c) KSL antimicrobial peptide solutions at concentrations ranging from 0 to 0.5 mg/mL.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4325973&req=5

fig2: Proliferation curves for MG-63 cells exposed to (a) Pac-525, (b) KSL-W, or (c) KSL antimicrobial peptide solutions at concentrations ranging from 0 to 0.5 mg/mL.
Mentions: Figure 2 shows the average cell proliferation curve, with the concentration of AMPs on the X-axis and OD values on the Y-axis. Compared with the untreated group, the absorbance values for each well (treated with Pac-525, KSL-W, or KSL) were similar for each concentration. There was no statistical difference between the AMP-treated group and the untreated group and no statistical difference among the eight concentrations of AMPs.

Bottom Line: The minimum inhibitory concentration and minimum bactericidal concentration values of Pac-525 were 0.0625 mg/mL and 0.125 mg/mL, respectively, for P. gingivalis and 0.0078 mg/mL and 0.0156 mg/mL, respectively, for F. nucleatum.Using CLSM, we confirmed that compared to 0.1% chlorhexidine, 0.5 mg/mL of Pac-525 caused a significant decrease in biofilm thickness and a decline in the percentage of live bacteria.These data indicate that Pac-525 has unique properties that might make it suitable for the inhibition the growth of pathogenic bacteria around dental implants.

View Article: PubMed Central - PubMed

Affiliation: Department of Prosthodontics, School of Stomatology, Dalian Medical University, Dalian 116044, China.

ABSTRACT
In order to investigate the potential of short antimicrobial peptides (AMPs) as alternative antibacterial agents during the treatment of peri-implantitis, the cytotoxic activity of three short AMPs, that is, Pac-525, KSL-W, and KSL, was determined using the MTT assay. The antimicrobial activity of these AMPs, ranging in concentration from 0.0039 mg/mL to 0.5 mg/mL, against the predominant planktonic pathogens, including Streptococcus sanguis, Fusobacterium nucleatum, and Porphyromonas gingivalis, involved in peri-implantitis was investigated. Furthermore, 2-day-old P. gingivalis biofilms cultured on titanium surfaces were treated with Pac-525 and subsequently observed and analysed using confocal laser scanning microscopy (CLSM). The average cell proliferation curve indicated that there was no cytotoxicity due to the three short AMPs. The minimum inhibitory concentration and minimum bactericidal concentration values of Pac-525 were 0.0625 mg/mL and 0.125 mg/mL, respectively, for P. gingivalis and 0.0078 mg/mL and 0.0156 mg/mL, respectively, for F. nucleatum. Using CLSM, we confirmed that compared to 0.1% chlorhexidine, 0.5 mg/mL of Pac-525 caused a significant decrease in biofilm thickness and a decline in the percentage of live bacteria. These data indicate that Pac-525 has unique properties that might make it suitable for the inhibition the growth of pathogenic bacteria around dental implants.

Show MeSH