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Clinical and laboratory features of seven patients with acute myeloid leukemia (AML)-M2/M3 and elevated myeloblasts and abnormal promyelocytes.

He G, Wang C, Li Q, Tan H, Chen F, Huang Z, Yu B, Zheng L, Zheng R, Liu D - Cancer Cell Int. (2014)

Bottom Line: The PML/RARα fusion gene was present in six patients and two patients presented a mixed PML/RARα and AML1/ETO genotype.Five cases achieved CR and two cases did not achieve remission and one case transform into AML-M2 after CR1.The clinical and laboratory features of seven patients with AML-M2/M3 are demonstrated in the present study, providing information on the FAB sub-classification.

View Article: PubMed Central - PubMed

Affiliation: Department of Hematology, The First Affiliated Hospital of Guangzhou Medical University, No. 1 Kangda Road, Haizhu District, 510000 Guangzhou, China.

ABSTRACT

Background: There is limited information on a special subtype of Acute myeloid leukemia (AML) characterized by >20% myeloblasts and >20% abnormal promyelocytes in bone marrow and peripheral blood.

Objective: The objective of the present investigation was to explore the clinical and laboratory features of seven patients with AML-M2/M3.

Method: We retrospectively assessed cell morphology, cytochemistry, immunophenotype, cytogenetics, and clinical features of seven patients with this rare subtype of AML.

Results: All seven cases had thrombocytopenia, coagulation abnormalities, >20% myeloblasts and abnormal promyelocytes. The PML/RARα fusion gene was present in six patients and two patients presented a mixed PML/RARα and AML1/ETO genotype. Five cases achieved CR and two cases did not achieve remission and one case transform into AML-M2 after CR1.

Conclusions: The clinical and laboratory features of seven patients with AML-M2/M3 are demonstrated in the present study, providing information on the FAB sub-classification.

No MeSH data available.


Related in: MedlinePlus

Morphological and immunological characteristics of BM cells in AML-M2/M3 patients. A, B: Bone marrow smears demonstrated hyperplasia of the myeloid lineage. Myeloblasts and abnormal promyelocytes accounted for >30% of BM cells. Abnormal promyelocytes were round, oval, or elliptical. The cytoplasm contained intense dust-like azurophilic granulation and some abnormal promyelocytes contained Auer rods. The nuclear size and shape were irregular; the nucleus could either appear kidney-shape, cerebriform, or even bi-lobed, with inconspicuous to prominent nucleoli. C: MPO: 99% positive; D: AS-DCE: 70% positive; E: NSE: particle positive; F: NSE positive cells were inhibited by NaF. G-L: Immunophenotype as determined by FCM for Case 7 showing two distinct groups of blast cells (red and green).
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Fig1: Morphological and immunological characteristics of BM cells in AML-M2/M3 patients. A, B: Bone marrow smears demonstrated hyperplasia of the myeloid lineage. Myeloblasts and abnormal promyelocytes accounted for >30% of BM cells. Abnormal promyelocytes were round, oval, or elliptical. The cytoplasm contained intense dust-like azurophilic granulation and some abnormal promyelocytes contained Auer rods. The nuclear size and shape were irregular; the nucleus could either appear kidney-shape, cerebriform, or even bi-lobed, with inconspicuous to prominent nucleoli. C: MPO: 99% positive; D: AS-DCE: 70% positive; E: NSE: particle positive; F: NSE positive cells were inhibited by NaF. G-L: Immunophenotype as determined by FCM for Case 7 showing two distinct groups of blast cells (red and green).

Mentions: Bone marrow smears showed hyperactivity of the myeloid lineage; myeloblasts and abnormal promyelocytes together comprised 100% of the BM cells. Abnormal promyelocytes, but with no Auer rods, accounted for 38% of all cells. Some myeloblasts exhibited one or two prominent nucleoli. Results of immunohistochemistry of BM biopsy tissue were consistent with AML: MPO(+++), CD117(+++), TdT(-), CD34(-), L26(-), UCHL-1(+),CD3(-), CD79a partial(+), Fe(-), and reticular fiber(-) (Figure 1).Figure 1


Clinical and laboratory features of seven patients with acute myeloid leukemia (AML)-M2/M3 and elevated myeloblasts and abnormal promyelocytes.

He G, Wang C, Li Q, Tan H, Chen F, Huang Z, Yu B, Zheng L, Zheng R, Liu D - Cancer Cell Int. (2014)

Morphological and immunological characteristics of BM cells in AML-M2/M3 patients. A, B: Bone marrow smears demonstrated hyperplasia of the myeloid lineage. Myeloblasts and abnormal promyelocytes accounted for >30% of BM cells. Abnormal promyelocytes were round, oval, or elliptical. The cytoplasm contained intense dust-like azurophilic granulation and some abnormal promyelocytes contained Auer rods. The nuclear size and shape were irregular; the nucleus could either appear kidney-shape, cerebriform, or even bi-lobed, with inconspicuous to prominent nucleoli. C: MPO: 99% positive; D: AS-DCE: 70% positive; E: NSE: particle positive; F: NSE positive cells were inhibited by NaF. G-L: Immunophenotype as determined by FCM for Case 7 showing two distinct groups of blast cells (red and green).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4325959&req=5

Fig1: Morphological and immunological characteristics of BM cells in AML-M2/M3 patients. A, B: Bone marrow smears demonstrated hyperplasia of the myeloid lineage. Myeloblasts and abnormal promyelocytes accounted for >30% of BM cells. Abnormal promyelocytes were round, oval, or elliptical. The cytoplasm contained intense dust-like azurophilic granulation and some abnormal promyelocytes contained Auer rods. The nuclear size and shape were irregular; the nucleus could either appear kidney-shape, cerebriform, or even bi-lobed, with inconspicuous to prominent nucleoli. C: MPO: 99% positive; D: AS-DCE: 70% positive; E: NSE: particle positive; F: NSE positive cells were inhibited by NaF. G-L: Immunophenotype as determined by FCM for Case 7 showing two distinct groups of blast cells (red and green).
Mentions: Bone marrow smears showed hyperactivity of the myeloid lineage; myeloblasts and abnormal promyelocytes together comprised 100% of the BM cells. Abnormal promyelocytes, but with no Auer rods, accounted for 38% of all cells. Some myeloblasts exhibited one or two prominent nucleoli. Results of immunohistochemistry of BM biopsy tissue were consistent with AML: MPO(+++), CD117(+++), TdT(-), CD34(-), L26(-), UCHL-1(+),CD3(-), CD79a partial(+), Fe(-), and reticular fiber(-) (Figure 1).Figure 1

Bottom Line: The PML/RARα fusion gene was present in six patients and two patients presented a mixed PML/RARα and AML1/ETO genotype.Five cases achieved CR and two cases did not achieve remission and one case transform into AML-M2 after CR1.The clinical and laboratory features of seven patients with AML-M2/M3 are demonstrated in the present study, providing information on the FAB sub-classification.

View Article: PubMed Central - PubMed

Affiliation: Department of Hematology, The First Affiliated Hospital of Guangzhou Medical University, No. 1 Kangda Road, Haizhu District, 510000 Guangzhou, China.

ABSTRACT

Background: There is limited information on a special subtype of Acute myeloid leukemia (AML) characterized by >20% myeloblasts and >20% abnormal promyelocytes in bone marrow and peripheral blood.

Objective: The objective of the present investigation was to explore the clinical and laboratory features of seven patients with AML-M2/M3.

Method: We retrospectively assessed cell morphology, cytochemistry, immunophenotype, cytogenetics, and clinical features of seven patients with this rare subtype of AML.

Results: All seven cases had thrombocytopenia, coagulation abnormalities, >20% myeloblasts and abnormal promyelocytes. The PML/RARα fusion gene was present in six patients and two patients presented a mixed PML/RARα and AML1/ETO genotype. Five cases achieved CR and two cases did not achieve remission and one case transform into AML-M2 after CR1.

Conclusions: The clinical and laboratory features of seven patients with AML-M2/M3 are demonstrated in the present study, providing information on the FAB sub-classification.

No MeSH data available.


Related in: MedlinePlus