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Proteome changes underpin improved meat quality and yield of chickens (Gallus gallus) fed the probiotic Enterococcus faecium.

Zheng A, Luo J, Meng K, Li J, Zhang S, Li K, Liu G, Cai H, Bryden WL, Yao B - BMC Genomics (2014)

Bottom Line: The probiotic significantly increased meat colour, water holding capacity and pH of pectoral muscle but decreased abdominal fat content.The validity of the proteomics results was further confirmed by qPCR.This study reveals that improved meat quality of broilers fed probiotics is triggered by proteome alterations (especially the glycolytic proteins), and provides a new insight into the mechanism by which probiotics improve poultry production.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Feed Biotechnology of Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, P, R, China. liuguohua@caas.cn.

ABSTRACT

Background: Supplementation of broiler chicken diets with probiotics may improve carcass characteristics and meat quality. However, the underlying molecular mechanism remains unclear. In the present study, 2D-DIGE-based proteomics was employed to investigate the proteome changes associated with improved carcass traits and meat quality of Arbor Acres broilers (Gallus gallus) fed the probiotic Enterococcus faecium.

Results: The probiotic significantly increased meat colour, water holding capacity and pH of pectoral muscle but decreased abdominal fat content. These meat quality changes were related to the altered abundance of 22 proteins in the pectoral muscle following E. faecium feeding. Of these, 17 proteins have central roles in regulating meat quality due to their biological interaction network. Altered cytoskeletal and chaperon protein expression also contribute to improved water holding capacity and colour of meat, which suggests that upregulation of chaperon proteins maintains cell integrity and prevents moisture loss by enhancing folding and recovery of the membrane and cytoskeletal proteins. The down-regulation of β-enolase and pyruvate kinase muscle isozymes suggests roles in increasing the pH of meat by decreasing the production of lactic acid. The validity of the proteomics results was further confirmed by qPCR.

Conclusions: This study reveals that improved meat quality of broilers fed probiotics is triggered by proteome alterations (especially the glycolytic proteins), and provides a new insight into the mechanism by which probiotics improve poultry production.

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Related in: MedlinePlus

Quantitative comparisons of the proteins of differential abundance from the pectoral muscles of 42-day-old AA broilers fed dietaryE. faecium. Av. ratio is the ratio of protein abundance of the treatment compared to the control groups. Positive values indicate the high protein abundance of the treatment group. PGM1, phosphoglucomutase-1; LDHA, L-lactate dehydrogenase A chain; CKMT2, mitochondrial creatine kinase; CKM, creatine kinase M-type; FBP2, fructose-1,6-bisphosphatase 2; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; PGK, phosphoglycerate kinase; PGAM1, phosphoglycerate mutase 1; ENO3, β-enolase; PKM2, pyruvate kinase muscle isozyme; MYH1, myosin, heavy chain 1; MYH2, myosin, heavy chain 2; MYH3, myosin-3; MYH6, myosin, heavy chain 6; MYH15, myosin, heavy chain 15; SSMHC, myosin, heavy chain 7B, beta; ACTN2, α-actinin-2; TTN, structural muscle protein titin; SM1, slow myosin heavy chain 1; HSP70, heat shock 70 kDa protein; ALB, albumin.
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Fig5: Quantitative comparisons of the proteins of differential abundance from the pectoral muscles of 42-day-old AA broilers fed dietaryE. faecium. Av. ratio is the ratio of protein abundance of the treatment compared to the control groups. Positive values indicate the high protein abundance of the treatment group. PGM1, phosphoglucomutase-1; LDHA, L-lactate dehydrogenase A chain; CKMT2, mitochondrial creatine kinase; CKM, creatine kinase M-type; FBP2, fructose-1,6-bisphosphatase 2; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; PGK, phosphoglycerate kinase; PGAM1, phosphoglycerate mutase 1; ENO3, β-enolase; PKM2, pyruvate kinase muscle isozyme; MYH1, myosin, heavy chain 1; MYH2, myosin, heavy chain 2; MYH3, myosin-3; MYH6, myosin, heavy chain 6; MYH15, myosin, heavy chain 15; SSMHC, myosin, heavy chain 7B, beta; ACTN2, α-actinin-2; TTN, structural muscle protein titin; SM1, slow myosin heavy chain 1; HSP70, heat shock 70 kDa protein; ALB, albumin.

Mentions: A comparison of proteins of differential abundance showed that more protein species were up-regulated in chickens fed E. faecium (16 of 22) (Figure 5). Of the 16 up-regulated protein species, nine proteins were involved in carbohydrate metabolism, five in cytoskeleton, one in chaperone protein and one in transporter. The four of six down-regulated proteins in chickens fed E. faecium were mainly related to cytoskeleton.Figure 5


Proteome changes underpin improved meat quality and yield of chickens (Gallus gallus) fed the probiotic Enterococcus faecium.

Zheng A, Luo J, Meng K, Li J, Zhang S, Li K, Liu G, Cai H, Bryden WL, Yao B - BMC Genomics (2014)

Quantitative comparisons of the proteins of differential abundance from the pectoral muscles of 42-day-old AA broilers fed dietaryE. faecium. Av. ratio is the ratio of protein abundance of the treatment compared to the control groups. Positive values indicate the high protein abundance of the treatment group. PGM1, phosphoglucomutase-1; LDHA, L-lactate dehydrogenase A chain; CKMT2, mitochondrial creatine kinase; CKM, creatine kinase M-type; FBP2, fructose-1,6-bisphosphatase 2; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; PGK, phosphoglycerate kinase; PGAM1, phosphoglycerate mutase 1; ENO3, β-enolase; PKM2, pyruvate kinase muscle isozyme; MYH1, myosin, heavy chain 1; MYH2, myosin, heavy chain 2; MYH3, myosin-3; MYH6, myosin, heavy chain 6; MYH15, myosin, heavy chain 15; SSMHC, myosin, heavy chain 7B, beta; ACTN2, α-actinin-2; TTN, structural muscle protein titin; SM1, slow myosin heavy chain 1; HSP70, heat shock 70 kDa protein; ALB, albumin.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4325948&req=5

Fig5: Quantitative comparisons of the proteins of differential abundance from the pectoral muscles of 42-day-old AA broilers fed dietaryE. faecium. Av. ratio is the ratio of protein abundance of the treatment compared to the control groups. Positive values indicate the high protein abundance of the treatment group. PGM1, phosphoglucomutase-1; LDHA, L-lactate dehydrogenase A chain; CKMT2, mitochondrial creatine kinase; CKM, creatine kinase M-type; FBP2, fructose-1,6-bisphosphatase 2; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; PGK, phosphoglycerate kinase; PGAM1, phosphoglycerate mutase 1; ENO3, β-enolase; PKM2, pyruvate kinase muscle isozyme; MYH1, myosin, heavy chain 1; MYH2, myosin, heavy chain 2; MYH3, myosin-3; MYH6, myosin, heavy chain 6; MYH15, myosin, heavy chain 15; SSMHC, myosin, heavy chain 7B, beta; ACTN2, α-actinin-2; TTN, structural muscle protein titin; SM1, slow myosin heavy chain 1; HSP70, heat shock 70 kDa protein; ALB, albumin.
Mentions: A comparison of proteins of differential abundance showed that more protein species were up-regulated in chickens fed E. faecium (16 of 22) (Figure 5). Of the 16 up-regulated protein species, nine proteins were involved in carbohydrate metabolism, five in cytoskeleton, one in chaperone protein and one in transporter. The four of six down-regulated proteins in chickens fed E. faecium were mainly related to cytoskeleton.Figure 5

Bottom Line: The probiotic significantly increased meat colour, water holding capacity and pH of pectoral muscle but decreased abdominal fat content.The validity of the proteomics results was further confirmed by qPCR.This study reveals that improved meat quality of broilers fed probiotics is triggered by proteome alterations (especially the glycolytic proteins), and provides a new insight into the mechanism by which probiotics improve poultry production.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Feed Biotechnology of Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, P, R, China. liuguohua@caas.cn.

ABSTRACT

Background: Supplementation of broiler chicken diets with probiotics may improve carcass characteristics and meat quality. However, the underlying molecular mechanism remains unclear. In the present study, 2D-DIGE-based proteomics was employed to investigate the proteome changes associated with improved carcass traits and meat quality of Arbor Acres broilers (Gallus gallus) fed the probiotic Enterococcus faecium.

Results: The probiotic significantly increased meat colour, water holding capacity and pH of pectoral muscle but decreased abdominal fat content. These meat quality changes were related to the altered abundance of 22 proteins in the pectoral muscle following E. faecium feeding. Of these, 17 proteins have central roles in regulating meat quality due to their biological interaction network. Altered cytoskeletal and chaperon protein expression also contribute to improved water holding capacity and colour of meat, which suggests that upregulation of chaperon proteins maintains cell integrity and prevents moisture loss by enhancing folding and recovery of the membrane and cytoskeletal proteins. The down-regulation of β-enolase and pyruvate kinase muscle isozymes suggests roles in increasing the pH of meat by decreasing the production of lactic acid. The validity of the proteomics results was further confirmed by qPCR.

Conclusions: This study reveals that improved meat quality of broilers fed probiotics is triggered by proteome alterations (especially the glycolytic proteins), and provides a new insight into the mechanism by which probiotics improve poultry production.

Show MeSH
Related in: MedlinePlus