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Coexpression analysis of nine neuropeptides in the neurosecretory preoptic area of larval zebrafish.

Herget U, Ryu S - Front Neuroanat (2015)

Bottom Line: To identify distinct cell types present in the larval NPO, we also generated a comprehensive 3D map of 9 zebrafish homologs of typical neuropeptides found in the mammalian PVN (arginine vasopressin (AVP), corticotropin-releasing hormone (CRH), proenkephalin a (penka)/b (penkb), neurotensin (NTS), oxytocin (OXT), vasoactive intestinal peptide (VIP), cholecystokinin (CCK), and somatostatin (SST)).Our results allowed the subclassification of NPO cell types, and differences in variability of coexpression profiles suggest potential targets of biochemical plasticity.Thus, this work provides an important basis for the analysis of the development, function, and plasticity of the primary neuroendocrine brain region in larval zebrafish.

View Article: PubMed Central - PubMed

Affiliation: Developmental Genetics of the Nervous System, Max Planck Institute for Medical Research Heidelberg, Germany ; The Hartmut Hoffmann-Berling International Graduate School of Molecular and Cellular Biology, University of Heidelberg Heidelberg, Germany.

ABSTRACT
The paraventricular nucleus (PVN) of the hypothalamus in mammals coordinates neuroendocrine, autonomic and behavioral responses pivotal for homeostasis and the stress response. A large amount of studies in rodents has documented that the PVN contains diverse neuronal cell types which can be identified by the expression of distinct secretory neuropeptides. Interestingly, PVN cell types often coexpress multiple neuropeptides whose relative coexpression levels are subject to environment-induced plasticity. Due to their small size and transparency, zebrafish larvae offer the possibility to comprehensively study the development and plasticity of the PVN in large groups of intact animals, yet important anatomical information about the larval zebrafish PVN-homologous region has been missing. Therefore we recently defined the location and borders of the larval neurosecretory preoptic area (NPO) as the PVN-homologous region in larval zebrafish based on transcription factor expression and cell type clustering. To identify distinct cell types present in the larval NPO, we also generated a comprehensive 3D map of 9 zebrafish homologs of typical neuropeptides found in the mammalian PVN (arginine vasopressin (AVP), corticotropin-releasing hormone (CRH), proenkephalin a (penka)/b (penkb), neurotensin (NTS), oxytocin (OXT), vasoactive intestinal peptide (VIP), cholecystokinin (CCK), and somatostatin (SST)). Here we extend this chemoarchitectural map to include the degrees of coexpression of two neuropeptides in the same cell by performing systematic pairwise comparisons. Our results allowed the subclassification of NPO cell types, and differences in variability of coexpression profiles suggest potential targets of biochemical plasticity. Thus, this work provides an important basis for the analysis of the development, function, and plasticity of the primary neuroendocrine brain region in larval zebrafish.

No MeSH data available.


Some peptide combinations show low or moderate coexpression. (A) Coexpression ((A) maximum intensity projection; (A’) single plane) of crh(A”) and penkb(A”’) rarely occurs, but can be found in more than one cell. (B) In some animals, coexpression ((B) maximum intensity projection; (B’) single plane) of penkb(B”) and avp(B”’) can be found. (C) Moderate coexpression ((C) maximum intensity projection; (C’) single plane) of sst1.1(C”) and crh(C”’) can be observed in some animals. (D) Few cells also show coexpression ((D) maximum intensity projection; (D’) single plane) of sst1.1(D”) and penkb(D”’). (E) Coexpression is found in few cells ((E) maximum intensity projection; (E’) single plane) labeled for sst1.1(E”) and avp(E”’). (F) In some animals, moderate coexpression ((F) maximum intensity projection; (F’) single plane) of penkb(F”) and oxt(F”’) can be found. Arrowheads mark coexpressing cells. Scale bar: 50 μm.
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Figure 4: Some peptide combinations show low or moderate coexpression. (A) Coexpression ((A) maximum intensity projection; (A’) single plane) of crh(A”) and penkb(A”’) rarely occurs, but can be found in more than one cell. (B) In some animals, coexpression ((B) maximum intensity projection; (B’) single plane) of penkb(B”) and avp(B”’) can be found. (C) Moderate coexpression ((C) maximum intensity projection; (C’) single plane) of sst1.1(C”) and crh(C”’) can be observed in some animals. (D) Few cells also show coexpression ((D) maximum intensity projection; (D’) single plane) of sst1.1(D”) and penkb(D”’). (E) Coexpression is found in few cells ((E) maximum intensity projection; (E’) single plane) labeled for sst1.1(E”) and avp(E”’). (F) In some animals, moderate coexpression ((F) maximum intensity projection; (F’) single plane) of penkb(F”) and oxt(F”’) can be found. Arrowheads mark coexpressing cells. Scale bar: 50 μm.

Mentions: The extent of coexpression for other peptide combinations was somewhat higher where more than one cell per animal showed coexpression. In the combination of crh and penkb staining, many animals showed no coexpression, but in one animal, coexpression was observed in few cells (Figures 4A–A”’, 1/12 animals). Similarly few avp and penkb coexpressing cells were found in some animals analyzed (Figures 4B–B”’, 5/15 animals). In those cases in which crh and sst1.1 were coexpressed, we found such coexpression in several cells (Figures 4C–C”’, 2/9 animals). Few cells coexpressing penkb and sst1.1 were also found in one animal (Figures 4D–D”’, 1/5 animals). Few avp and sst1.1 coexpressing cells were also found in some animals (Figures 4E–E”’, 6/25 animals). Some animals showed few oxt and penkb coexpressing cells (Figures 4F–F”’, 4/7 animals). Although in some animals, penka and nts were not coexpressed, in most animals we found coexpression of these peptides in few cells (Figures 5A–A”’, 8/15 animals).


Coexpression analysis of nine neuropeptides in the neurosecretory preoptic area of larval zebrafish.

Herget U, Ryu S - Front Neuroanat (2015)

Some peptide combinations show low or moderate coexpression. (A) Coexpression ((A) maximum intensity projection; (A’) single plane) of crh(A”) and penkb(A”’) rarely occurs, but can be found in more than one cell. (B) In some animals, coexpression ((B) maximum intensity projection; (B’) single plane) of penkb(B”) and avp(B”’) can be found. (C) Moderate coexpression ((C) maximum intensity projection; (C’) single plane) of sst1.1(C”) and crh(C”’) can be observed in some animals. (D) Few cells also show coexpression ((D) maximum intensity projection; (D’) single plane) of sst1.1(D”) and penkb(D”’). (E) Coexpression is found in few cells ((E) maximum intensity projection; (E’) single plane) labeled for sst1.1(E”) and avp(E”’). (F) In some animals, moderate coexpression ((F) maximum intensity projection; (F’) single plane) of penkb(F”) and oxt(F”’) can be found. Arrowheads mark coexpressing cells. Scale bar: 50 μm.
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Related In: Results  -  Collection

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Figure 4: Some peptide combinations show low or moderate coexpression. (A) Coexpression ((A) maximum intensity projection; (A’) single plane) of crh(A”) and penkb(A”’) rarely occurs, but can be found in more than one cell. (B) In some animals, coexpression ((B) maximum intensity projection; (B’) single plane) of penkb(B”) and avp(B”’) can be found. (C) Moderate coexpression ((C) maximum intensity projection; (C’) single plane) of sst1.1(C”) and crh(C”’) can be observed in some animals. (D) Few cells also show coexpression ((D) maximum intensity projection; (D’) single plane) of sst1.1(D”) and penkb(D”’). (E) Coexpression is found in few cells ((E) maximum intensity projection; (E’) single plane) labeled for sst1.1(E”) and avp(E”’). (F) In some animals, moderate coexpression ((F) maximum intensity projection; (F’) single plane) of penkb(F”) and oxt(F”’) can be found. Arrowheads mark coexpressing cells. Scale bar: 50 μm.
Mentions: The extent of coexpression for other peptide combinations was somewhat higher where more than one cell per animal showed coexpression. In the combination of crh and penkb staining, many animals showed no coexpression, but in one animal, coexpression was observed in few cells (Figures 4A–A”’, 1/12 animals). Similarly few avp and penkb coexpressing cells were found in some animals analyzed (Figures 4B–B”’, 5/15 animals). In those cases in which crh and sst1.1 were coexpressed, we found such coexpression in several cells (Figures 4C–C”’, 2/9 animals). Few cells coexpressing penkb and sst1.1 were also found in one animal (Figures 4D–D”’, 1/5 animals). Few avp and sst1.1 coexpressing cells were also found in some animals (Figures 4E–E”’, 6/25 animals). Some animals showed few oxt and penkb coexpressing cells (Figures 4F–F”’, 4/7 animals). Although in some animals, penka and nts were not coexpressed, in most animals we found coexpression of these peptides in few cells (Figures 5A–A”’, 8/15 animals).

Bottom Line: To identify distinct cell types present in the larval NPO, we also generated a comprehensive 3D map of 9 zebrafish homologs of typical neuropeptides found in the mammalian PVN (arginine vasopressin (AVP), corticotropin-releasing hormone (CRH), proenkephalin a (penka)/b (penkb), neurotensin (NTS), oxytocin (OXT), vasoactive intestinal peptide (VIP), cholecystokinin (CCK), and somatostatin (SST)).Our results allowed the subclassification of NPO cell types, and differences in variability of coexpression profiles suggest potential targets of biochemical plasticity.Thus, this work provides an important basis for the analysis of the development, function, and plasticity of the primary neuroendocrine brain region in larval zebrafish.

View Article: PubMed Central - PubMed

Affiliation: Developmental Genetics of the Nervous System, Max Planck Institute for Medical Research Heidelberg, Germany ; The Hartmut Hoffmann-Berling International Graduate School of Molecular and Cellular Biology, University of Heidelberg Heidelberg, Germany.

ABSTRACT
The paraventricular nucleus (PVN) of the hypothalamus in mammals coordinates neuroendocrine, autonomic and behavioral responses pivotal for homeostasis and the stress response. A large amount of studies in rodents has documented that the PVN contains diverse neuronal cell types which can be identified by the expression of distinct secretory neuropeptides. Interestingly, PVN cell types often coexpress multiple neuropeptides whose relative coexpression levels are subject to environment-induced plasticity. Due to their small size and transparency, zebrafish larvae offer the possibility to comprehensively study the development and plasticity of the PVN in large groups of intact animals, yet important anatomical information about the larval zebrafish PVN-homologous region has been missing. Therefore we recently defined the location and borders of the larval neurosecretory preoptic area (NPO) as the PVN-homologous region in larval zebrafish based on transcription factor expression and cell type clustering. To identify distinct cell types present in the larval NPO, we also generated a comprehensive 3D map of 9 zebrafish homologs of typical neuropeptides found in the mammalian PVN (arginine vasopressin (AVP), corticotropin-releasing hormone (CRH), proenkephalin a (penka)/b (penkb), neurotensin (NTS), oxytocin (OXT), vasoactive intestinal peptide (VIP), cholecystokinin (CCK), and somatostatin (SST)). Here we extend this chemoarchitectural map to include the degrees of coexpression of two neuropeptides in the same cell by performing systematic pairwise comparisons. Our results allowed the subclassification of NPO cell types, and differences in variability of coexpression profiles suggest potential targets of biochemical plasticity. Thus, this work provides an important basis for the analysis of the development, function, and plasticity of the primary neuroendocrine brain region in larval zebrafish.

No MeSH data available.