The Dictyostelium prestalk inducer differentiation-inducing factor-1 (DIF-1) triggers unexpectedly complex global phosphorylation changes.
Bottom Line: The results also provide evidence that the Ca(2+)/calmodulin-dependent phosphatase calcineurin plays a role in DIF-1 signaling to the DimB prestalk transcription factor.This accords with studies that suggest an antagonism between the two inducers and also with the rapid dephosphorylation of the cAMP receptor that we observe in response to DIF-1 and with the known inhibitory effect of DIF-1 on chemotaxis to cAMP.All MS data are available via ProteomeXchange with identifier PXD001555.
Affiliation: College of Life Sciences, University of Dundee, Dundee DD1 5EH, United Kingdom.Show MeSH
Mentions: An interesting temporal pattern emerged when class I phosphorylation sites were analyzed by cluster analysis (Saeed et al., 2011) to detect groups of sites that change their phosphorylation with similar temporal profiles. Clusters showing dephosphorylation contained many more sites, indicating that DIF-1 may trigger a net dephosphorylation of cellular proteins (Figure 1C). A similar pattern emerges when class II sites are grouped into either phosphorylation or dephosphorylation events. The balance of phosphorylation versus dephosphorylation events dramatically shifts; at 1 min, just 39% of the class II sites are dephosphorylations, increasing to 94% at 15 min (Figure 2A).
Affiliation: College of Life Sciences, University of Dundee, Dundee DD1 5EH, United Kingdom.