The volumes and transcript counts of single cells reveal concentration homeostasis and capture biological noise.
Bottom Line: Cell-to-cell variability is best captured in terms of concentration rather than molecule counts, because reaction rates depend on concentrations.We compared three cell clones that differ only in the genomic integration site of an identical constitutively expressed reporter gene.This study highlights the importance of the quantitative measurement of transcript concentrations in studies of cell-to-cell variability in biology.
Affiliation: Synthetic Systems Biology and Nuclear Organization Group, Swammerdam Institute for Life Sciences, University of Amsterdam, 1098 XH Amsterdam, The Netherlands.Show MeSH
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Mentions: Figure 4B makes the same decomposition for the experimental data. It shows that the gene expression noise term accounts for ∼70% of the mRNA number noise and for greater than 95% of the mRNA concentration noise. This indicates that mRNA number noise has a large contribution that derives from the scaling of the number of transcripts per cell with the cell volume.
Affiliation: Synthetic Systems Biology and Nuclear Organization Group, Swammerdam Institute for Life Sciences, University of Amsterdam, 1098 XH Amsterdam, The Netherlands.