Seipin performs dissectible functions in promoting lipid droplet biogenesis and regulating droplet morphology.
Bottom Line: Furthermore, we find that the normal rate of droplet initiation depends on 14 amino acids at the amino terminus of seipin, deletion of which results in fewer, larger droplets that are consistent with a delay in initiation but are otherwise normal in morphology.Importantly, other functions of seipin, namely vectorial budding and resistance to inositol, are retained in this mutant.We conclude that seipin has dissectible roles in both promoting early LD initiation and in regulating LD morphology, supporting its importance in LD biogenesis.
Affiliation: Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, TX 75235-9041.Show MeSH
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Mentions: Cells were analyzed at 0, 3, 6, and 9 h after the switch to galactose. Micrographs at 0 h revealed no BODIPY staining, except for a single droplet in a rare 3KO(GALDGA1) cell, while 82% of cells in this strain produced droplets after only 3 h; this number increased to 98% by 9 h after induction (Figure 1, A–C). By 9 h, cells typically contained approximately five droplets (Figure 1D).
Affiliation: Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, TX 75235-9041.