FAP206 is a microtubule-docking adapter for ciliary radial spoke 2 and dynein c.
Bottom Line: A knockout of the conserved ciliary protein FAP206 in the ciliate Tetrahymena resulted in slow cell motility.Cryo-electron tomography showed that in the absence of FAP206, the 96-nm repeats lacked RS2 and dynein c.Overexpressed GFP-FAP206 decorated nonciliary microtubules in vivo.
Affiliation: Department of Cellular Biology, University of Georgia, Athens, GA 30602.Show MeSH
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Mentions: To localize FAP206 under native conditions of expression, we tagged the FAP206 gene with a sequence encoding a C-terminal green fluorescent protein (GFP). The gross phenotype of the FAP206-GFP strain appeared normal. In both live (Figure 1A and Supplemental Movie S1) and detergent-treated (Triton X-100) cells (Figure 1B), FAP206-GFP was detected exclusively in cilia, where it was distributed uniformly. The detergent resistance indicates that FAP206 is stably associated with the axoneme, in agreement with published biochemical studies (Pazour et al., 2005; Lin et al., 2011; Gupta et al., 2012).
Affiliation: Department of Cellular Biology, University of Georgia, Athens, GA 30602.