c-di-GMP induction of Dictyostelium cell death requires the polyketide DIF-1.
Bottom Line: In these cases, c-di-GMP-induced cell death was rescued by complementation with exogenous DIF-1.Taken together, these results demonstrated that c-di-GMP could trigger cell death in Dictyostelium only in the presence of the DIF-1 polyketide or its metabolites.This identified another element of control to this cell death and perhaps also to c-di-GMP effects in other situations and organisms.
Affiliation: Centre d'Immunologie de Marseille-Luminy, UM2 Aix-Marseille Université; Institut National de la Santé et de la Recherche Médicale, U1104; and Centre National de la Recherche Scientifique, UMR7280, 13288 Marseille, France.Show MeSH
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Mentions: We first used Dictyostelium HMX44A cells, which are known to make very little DIF-1 but to remain responsive to exogenous DIF-1 (Kopachik et al., 1983). Accordingly, these cells vacuolized well with exogenous DIF-1 (Figure 2A). In contrast, addition of exogenous c-di-GMP led to small, roundish, contrasted cells with almost no vacuolization (Figure 2A), no cellulose encasings (unpublished data), and no death when tested by regrowth (Figure 2B, right). In addition, in these HMX44A cells, where exogenous c-di-GMP alone did not induce cell death, there was synergy between exogenous DIF-1 and exogenous c-di-GMP (Figure 2C). Taken together, the results indicate that in HMX44A cells relative to DH1 cells, an unknown mutation, or mutations, prevented vacuolar cell death induced by c-di-GMP. Again, irrespective of their other possible defects, HMX44A cells produced no or little DIF-1. These results were in line with the possibility of a requirement of DIF-1 for c-di-GMP induction of cell death.
Affiliation: Centre d'Immunologie de Marseille-Luminy, UM2 Aix-Marseille Université; Institut National de la Santé et de la Recherche Médicale, U1104; and Centre National de la Recherche Scientifique, UMR7280, 13288 Marseille, France.