Arrestins regulate cell spreading and motility via focal adhesion dynamics.
Bottom Line: Clathrin exhibited decreased dynamics near FA in arrestin-deficient cells.In contrast to wild-type arrestins, mutants deficient in clathrin binding did not rescue the phenotype.Collectively the data indicate that arrestins are key regulators of FA disassembly linking microtubules and clathrin.
Affiliation: Department of Pharmacology, Vanderbilt University, Nashville, TN 37232.Show MeSH
Related in: MedlinePlus
Mentions: Cytoskeletal rearrangements drive cell movement and adhesion. Therefore we tested whether the lack of arrestins affects adhesion and migration. The adhesion of DKO cells was similar to WT after initial attachment (15 min; Figure 2A), but they adhered significantly better than WT as cells began to spread (30 min; Figure 2B). Thus DKO cells form initial attachments similar to WT but later demonstrate enhanced adhesion. Moreover, DKO cells demonstrated 3.8-fold- reduced migration toward the FN substrate in a Transwell assay (Figure 2D).
Affiliation: Department of Pharmacology, Vanderbilt University, Nashville, TN 37232.