Arrestins regulate cell spreading and motility via focal adhesion dynamics.
Bottom Line: Clathrin exhibited decreased dynamics near FA in arrestin-deficient cells.In contrast to wild-type arrestins, mutants deficient in clathrin binding did not rescue the phenotype.Collectively the data indicate that arrestins are key regulators of FA disassembly linking microtubules and clathrin.
Affiliation: Department of Pharmacology, Vanderbilt University, Nashville, TN 37232.Show MeSH
Related in: MedlinePlus
Mentions: Double arrestin-2/3 knockout (DKO) mouse embryonic fibroblasts (MEFs) were introduced more than a decade ago (Kohout et al., 2001), but their larger size and peculiar shape, dramatically different from that of wild-type (WT) MEFs, were routinely ignored. The actin cytoskeleton of arrestin DKO cells plated on fibronectin (FN) was drastically different from that in WT (Figure 1A), and DKO cells were twice as large (Figure 1B). DKO cell spreading on poly-d-lysine (PDL), which binds integrins but does not promote their clustering and activation, was similar to that on FN (Figure 1, A and B). In contrast, WT cells do not spread well on PDL: the average cell area was reduced nearly by half (Figure 1B).
Affiliation: Department of Pharmacology, Vanderbilt University, Nashville, TN 37232.