Nup2 requires a highly divergent partner, NupA, to fulfill functions at nuclear pore complexes and the mitotic chromatin region.
Bottom Line: These mitotic problems are not caused by overall defects in mitotic NPC disassembly-reassembly or general nuclear import.However, without Nup2 or NupA, although the SAC protein Mad1 locates to its mitotic locations, it fails to locate to NPCs normally in G1 after mitosis.Collectively the study provides new insight into the roles of Nup2 and NupA during mitosis and in a surveillance mechanism that regulates nucleokinesis when mitotic defects occur after SAC fulfillment.
Affiliation: Laboratory of Gene Regulation and Development, National Institute for Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892.Show MeSH
Related in: MedlinePlus
Mentions: Endogenous Nup2 tagged with green fluorescent protein (GFP) is functional and translocates from NPCs to the chromatin region during mitotic entry and relocates back to NPCs during mitotic exit (Osmani et al., 2006a). To determine whether Nup2 is located like Nup2-GFP, an antibody was generated and used for immunofluorescence microscopy. Similar to the GFP-tagged version, untagged Nup2 was found to locate to NPCs during interphase and the chromatin region during mitosis (Figure 1, A and B), indicating Nup2-GFP reflects the normal cell cycle– regulated locations of Nup2.
Affiliation: Laboratory of Gene Regulation and Development, National Institute for Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892.