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Klebsiella pneumoniae carbapenemase (KPC)-producing K. pneumoniae at a single institution: insights into endemicity from whole-genome sequencing.

Mathers AJ, Stoesser N, Sheppard AE, Pankhurst L, Giess A, Yeh AJ, Didelot X, Turner SD, Sebra R, Kasarskis A, Peto T, Crook D, Sifri CD - Antimicrob. Agents Chemother. (2015)

Bottom Line: Using WGS-based analysis of clinical isolates and plasmid transformants, we demonstrate the unexpected dispersal of blaKPC to many non-ST258 lineages in a hospital through spread of at least two novel blaKPC plasmids.In contrast, ST258 KPC-Kp was imported into the institution on numerous occasions, with other blaKPC plasmid vectors and without sustained transmission.Instead, a newly recognized KPC-Kp strain, ST941, became associated with both novel blaKPC plasmids and spread locally, making it a future candidate for clinical persistence and dissemination.

View Article: PubMed Central - PubMed

Affiliation: Division of Infectious Diseases and International Health, Department of Medicine, University of Virginia Health System, Charlottesville, Virginia, USA Clinical Microbiology, Department of Pathology, University of Virginia Health System, Charlottesville, Virginia, USA ajm5b@virginia.edu.

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Annotated novel blaKPC plasmids pKPC_UVA01 and pKPC_UVA02 from Klebsiella pneumoniae and Klebsiella oxytoca isolated from the index patient.
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Figure 5: Annotated novel blaKPC plasmids pKPC_UVA01 and pKPC_UVA02 from Klebsiella pneumoniae and Klebsiella oxytoca isolated from the index patient.

Mentions: We previously described an index case with KPC-Kp (CAV1016) who was admitted to the UVaMC with subsequent blaKPC plasmid dispersal to other Enterobacteriaceae (4). De novo assembly of the index blaKPC plasmid from CAV1016 was generated from the E. coli GeneHogs transformant (GH1016). It produced a 43,621-bp, closed, nontypeable (by incompatibility group) Tn4401-containing plasmid (pKPC_UVA01) with little homology to any previously described plasmids (GenBank accession no. CP009465) (Fig. 5).


Klebsiella pneumoniae carbapenemase (KPC)-producing K. pneumoniae at a single institution: insights into endemicity from whole-genome sequencing.

Mathers AJ, Stoesser N, Sheppard AE, Pankhurst L, Giess A, Yeh AJ, Didelot X, Turner SD, Sebra R, Kasarskis A, Peto T, Crook D, Sifri CD - Antimicrob. Agents Chemother. (2015)

Annotated novel blaKPC plasmids pKPC_UVA01 and pKPC_UVA02 from Klebsiella pneumoniae and Klebsiella oxytoca isolated from the index patient.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4325807&req=5

Figure 5: Annotated novel blaKPC plasmids pKPC_UVA01 and pKPC_UVA02 from Klebsiella pneumoniae and Klebsiella oxytoca isolated from the index patient.
Mentions: We previously described an index case with KPC-Kp (CAV1016) who was admitted to the UVaMC with subsequent blaKPC plasmid dispersal to other Enterobacteriaceae (4). De novo assembly of the index blaKPC plasmid from CAV1016 was generated from the E. coli GeneHogs transformant (GH1016). It produced a 43,621-bp, closed, nontypeable (by incompatibility group) Tn4401-containing plasmid (pKPC_UVA01) with little homology to any previously described plasmids (GenBank accession no. CP009465) (Fig. 5).

Bottom Line: Using WGS-based analysis of clinical isolates and plasmid transformants, we demonstrate the unexpected dispersal of blaKPC to many non-ST258 lineages in a hospital through spread of at least two novel blaKPC plasmids.In contrast, ST258 KPC-Kp was imported into the institution on numerous occasions, with other blaKPC plasmid vectors and without sustained transmission.Instead, a newly recognized KPC-Kp strain, ST941, became associated with both novel blaKPC plasmids and spread locally, making it a future candidate for clinical persistence and dissemination.

View Article: PubMed Central - PubMed

Affiliation: Division of Infectious Diseases and International Health, Department of Medicine, University of Virginia Health System, Charlottesville, Virginia, USA Clinical Microbiology, Department of Pathology, University of Virginia Health System, Charlottesville, Virginia, USA ajm5b@virginia.edu.

Show MeSH
Related in: MedlinePlus