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Klebsiella pneumoniae carbapenemase (KPC)-producing K. pneumoniae at a single institution: insights into endemicity from whole-genome sequencing.

Mathers AJ, Stoesser N, Sheppard AE, Pankhurst L, Giess A, Yeh AJ, Didelot X, Turner SD, Sebra R, Kasarskis A, Peto T, Crook D, Sifri CD - Antimicrob. Agents Chemother. (2015)

Bottom Line: Using WGS-based analysis of clinical isolates and plasmid transformants, we demonstrate the unexpected dispersal of blaKPC to many non-ST258 lineages in a hospital through spread of at least two novel blaKPC plasmids.In contrast, ST258 KPC-Kp was imported into the institution on numerous occasions, with other blaKPC plasmid vectors and without sustained transmission.Instead, a newly recognized KPC-Kp strain, ST941, became associated with both novel blaKPC plasmids and spread locally, making it a future candidate for clinical persistence and dissemination.

View Article: PubMed Central - PubMed

Affiliation: Division of Infectious Diseases and International Health, Department of Medicine, University of Virginia Health System, Charlottesville, Virginia, USA Clinical Microbiology, Department of Pathology, University of Virginia Health System, Charlottesville, Virginia, USA ajm5b@virginia.edu.

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Time-scaled representation of genetic relationships between multilocus sequence type ST941 strains, in association with predicted Klebsiella pneumoniae carbapenemase plasmids (pKPC_UVA01 and pKPC_UVA02). The numbers of mutational substitutions are represented numerically on the branches, with bars at nodes indicating 95% credibility intervals around the estimates of the time to the most recent common ancestors.
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Figure 3: Time-scaled representation of genetic relationships between multilocus sequence type ST941 strains, in association with predicted Klebsiella pneumoniae carbapenemase plasmids (pKPC_UVA01 and pKPC_UVA02). The numbers of mutational substitutions are represented numerically on the branches, with bars at nodes indicating 95% credibility intervals around the estimates of the time to the most recent common ancestors.

Mentions: Based on a ClonalFrame host strain analysis of the ST941 isolates, the TMRCA dated to mid-2006 with the 95% credibility interval ranging from 2003 to 2008 (Fig. 3). This includes the time of first appearance in 2007 of blaKPC in UVaMC and would be consistent with this lineage acquiring blaKPC soon after its appearance in the local hospital ecosystem.


Klebsiella pneumoniae carbapenemase (KPC)-producing K. pneumoniae at a single institution: insights into endemicity from whole-genome sequencing.

Mathers AJ, Stoesser N, Sheppard AE, Pankhurst L, Giess A, Yeh AJ, Didelot X, Turner SD, Sebra R, Kasarskis A, Peto T, Crook D, Sifri CD - Antimicrob. Agents Chemother. (2015)

Time-scaled representation of genetic relationships between multilocus sequence type ST941 strains, in association with predicted Klebsiella pneumoniae carbapenemase plasmids (pKPC_UVA01 and pKPC_UVA02). The numbers of mutational substitutions are represented numerically on the branches, with bars at nodes indicating 95% credibility intervals around the estimates of the time to the most recent common ancestors.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4325807&req=5

Figure 3: Time-scaled representation of genetic relationships between multilocus sequence type ST941 strains, in association with predicted Klebsiella pneumoniae carbapenemase plasmids (pKPC_UVA01 and pKPC_UVA02). The numbers of mutational substitutions are represented numerically on the branches, with bars at nodes indicating 95% credibility intervals around the estimates of the time to the most recent common ancestors.
Mentions: Based on a ClonalFrame host strain analysis of the ST941 isolates, the TMRCA dated to mid-2006 with the 95% credibility interval ranging from 2003 to 2008 (Fig. 3). This includes the time of first appearance in 2007 of blaKPC in UVaMC and would be consistent with this lineage acquiring blaKPC soon after its appearance in the local hospital ecosystem.

Bottom Line: Using WGS-based analysis of clinical isolates and plasmid transformants, we demonstrate the unexpected dispersal of blaKPC to many non-ST258 lineages in a hospital through spread of at least two novel blaKPC plasmids.In contrast, ST258 KPC-Kp was imported into the institution on numerous occasions, with other blaKPC plasmid vectors and without sustained transmission.Instead, a newly recognized KPC-Kp strain, ST941, became associated with both novel blaKPC plasmids and spread locally, making it a future candidate for clinical persistence and dissemination.

View Article: PubMed Central - PubMed

Affiliation: Division of Infectious Diseases and International Health, Department of Medicine, University of Virginia Health System, Charlottesville, Virginia, USA Clinical Microbiology, Department of Pathology, University of Virginia Health System, Charlottesville, Virginia, USA ajm5b@virginia.edu.

Show MeSH
Related in: MedlinePlus