Limits...
Klebsiella pneumoniae carbapenemase (KPC)-producing K. pneumoniae at a single institution: insights into endemicity from whole-genome sequencing.

Mathers AJ, Stoesser N, Sheppard AE, Pankhurst L, Giess A, Yeh AJ, Didelot X, Turner SD, Sebra R, Kasarskis A, Peto T, Crook D, Sifri CD - Antimicrob. Agents Chemother. (2015)

Bottom Line: Using WGS-based analysis of clinical isolates and plasmid transformants, we demonstrate the unexpected dispersal of blaKPC to many non-ST258 lineages in a hospital through spread of at least two novel blaKPC plasmids.In contrast, ST258 KPC-Kp was imported into the institution on numerous occasions, with other blaKPC plasmid vectors and without sustained transmission.Instead, a newly recognized KPC-Kp strain, ST941, became associated with both novel blaKPC plasmids and spread locally, making it a future candidate for clinical persistence and dissemination.

View Article: PubMed Central - PubMed

Affiliation: Division of Infectious Diseases and International Health, Department of Medicine, University of Virginia Health System, Charlottesville, Virginia, USA Clinical Microbiology, Department of Pathology, University of Virginia Health System, Charlottesville, Virginia, USA ajm5b@virginia.edu.

Show MeSH

Related in: MedlinePlus

Molecular clock estimate calculated using Bayesian inference on genetic data from the first and last Klebsiella pneumoniae isolates producing K. pneumoniae carbapenemase sampled from study individuals. The mean molecular clock estimate (solid line) and 95% credibility interval (dashed lines) are shown.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4325807&req=5

Figure 2: Molecular clock estimate calculated using Bayesian inference on genetic data from the first and last Klebsiella pneumoniae isolates producing K. pneumoniae carbapenemase sampled from study individuals. The mean molecular clock estimate (solid line) and 95% credibility interval (dashed lines) are shown.

Mentions: Data from 11 individuals with paired longitudinal samples sharing the same ST were appropriate for the assessment of the molecular clock, with a median of 18 days (range, 0 to 274 days) and 1 SNV (range, 0 to 10 SNVs) between paired samples. The molecular clock was calculated as being 1.9 × 10−6 substitutions/called site/year (95% credibility interval [95% CI], 1.1 × 10−6 to 2.9 × 10−6), equating to 10.1 substitutions/genome/year (95% CI, 5.7 to 15.6) (Fig. 2).


Klebsiella pneumoniae carbapenemase (KPC)-producing K. pneumoniae at a single institution: insights into endemicity from whole-genome sequencing.

Mathers AJ, Stoesser N, Sheppard AE, Pankhurst L, Giess A, Yeh AJ, Didelot X, Turner SD, Sebra R, Kasarskis A, Peto T, Crook D, Sifri CD - Antimicrob. Agents Chemother. (2015)

Molecular clock estimate calculated using Bayesian inference on genetic data from the first and last Klebsiella pneumoniae isolates producing K. pneumoniae carbapenemase sampled from study individuals. The mean molecular clock estimate (solid line) and 95% credibility interval (dashed lines) are shown.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4325807&req=5

Figure 2: Molecular clock estimate calculated using Bayesian inference on genetic data from the first and last Klebsiella pneumoniae isolates producing K. pneumoniae carbapenemase sampled from study individuals. The mean molecular clock estimate (solid line) and 95% credibility interval (dashed lines) are shown.
Mentions: Data from 11 individuals with paired longitudinal samples sharing the same ST were appropriate for the assessment of the molecular clock, with a median of 18 days (range, 0 to 274 days) and 1 SNV (range, 0 to 10 SNVs) between paired samples. The molecular clock was calculated as being 1.9 × 10−6 substitutions/called site/year (95% credibility interval [95% CI], 1.1 × 10−6 to 2.9 × 10−6), equating to 10.1 substitutions/genome/year (95% CI, 5.7 to 15.6) (Fig. 2).

Bottom Line: Using WGS-based analysis of clinical isolates and plasmid transformants, we demonstrate the unexpected dispersal of blaKPC to many non-ST258 lineages in a hospital through spread of at least two novel blaKPC plasmids.In contrast, ST258 KPC-Kp was imported into the institution on numerous occasions, with other blaKPC plasmid vectors and without sustained transmission.Instead, a newly recognized KPC-Kp strain, ST941, became associated with both novel blaKPC plasmids and spread locally, making it a future candidate for clinical persistence and dissemination.

View Article: PubMed Central - PubMed

Affiliation: Division of Infectious Diseases and International Health, Department of Medicine, University of Virginia Health System, Charlottesville, Virginia, USA Clinical Microbiology, Department of Pathology, University of Virginia Health System, Charlottesville, Virginia, USA ajm5b@virginia.edu.

Show MeSH
Related in: MedlinePlus