Chicken and duck myotubes are highly susceptible and permissive to influenza virus infection.
Bottom Line: Besides its primary function in movement and posture, skeletal muscle is a significant innate immune organ with the capacity to produce cytokines and chemokines and respond to proinflammatory cytokines.Infected chicken myotubes produced significantly higher levels of proinflammatory cytokines than did the corresponding duck cells.Our results indicate that avian skeletal muscle fibers of chicken and duck could be significant contributors to progeny production of highly pathogenic H5N1 viruses.
Affiliation: School of Veterinary Medicine and Science, University of Nottingham, Loughborough, Leicestershire, United Kingdom.Show MeSH
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Mentions: Chicken and duck myotubes were infected with three different avian influenza viruses (LPAI H2N3, HPAI H5N1 50-92, and HPAI H5N1 tyTy05) at a MOI of 1.0 over a period of 24 h. Viral M gene RNA accumulation, normalized to 18S rRNA, in duck myotubes was consistently higher than that in the corresponding chicken myotubes for all three viruses (Fig. 3A); a similar relative difference in M gene expression was also found for influenza virus-infected duck and chicken primary fibroblasts (our unpublished data). However, both chicken and duck myotubes infected with H5N1 tyTy05 virus at a MOI of 1.0 produced comparable increasing levels of progeny virus from 8 to 24 h of infection (Fig. 3A). MTT assays to determine the resulting metabolic rates (cell viability) at 24 h p.i. showed similar reductions in infected chicken and infected duck myotubes (Fig. 3B). Therefore, infected chicken and duck myotubes showed similar reductions in cell viability and were comparably permissive to the production of viable H5N1 tyTy05 progeny virus.
Affiliation: School of Veterinary Medicine and Science, University of Nottingham, Loughborough, Leicestershire, United Kingdom.