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Analysis of the early immune response to infection by infectious bursal disease virus in chickens differing in their resistance to the disease.

Smith J, Sadeyen JR, Butter C, Kaiser P, Burt DW - J. Virol. (2014)

Bottom Line: There is thus an urgent need to explore new control solutions, one of which would be to breed birds with greater resistance to IBD.This goal is perhaps uniquely achievable with poultry, of all farm animal species, since the genetics of 85% of the 60 billion chickens produced worldwide each year is under the control of essentially two breeding companies.In a comprehensive study, we attempt here to identify global transcriptomic differences in the target organ of the virus between chicken lines that differ in resistance and to predict candidate resistance genes.

View Article: PubMed Central - PubMed

Affiliation: The Roslin Institute and R(D)SVS, University of Edinburgh, Easter Bush, Midlothian, United Kingdom jacqueline.smith@roslin.ed.ac.uk.

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qRT-PCR analysis of 20 genes differentially expressed during IBDV infection. (A) Representative genes with inherent differences in expression between susceptible and resistant control birds. (B) Representative genes with expression changes during the host response in the susceptible line. (C) Representative genes with differential gene expression between the susceptible and resistant lines during the host response.
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Figure 7: qRT-PCR analysis of 20 genes differentially expressed during IBDV infection. (A) Representative genes with inherent differences in expression between susceptible and resistant control birds. (B) Representative genes with expression changes during the host response in the susceptible line. (C) Representative genes with differential gene expression between the susceptible and resistant lines during the host response.

Mentions: Twenty-one genes were chosen for verification by qRT-PCR, including genes involved in the host response and genes differentially expressed between the susceptible and resistant lines (either inherently or during the course of infection) (Table 4). Of the 20 genes tested, differential expression in the microarray was confirmed in the qRT-PCR analysis for 17. However, the microarray results for SRFBP1, TNFRSF1B, and CARD9 were not replicated in the qRT-PCR analysis (note that the qRT-PCR assay did not work for BLB1) (Fig. 7).


Analysis of the early immune response to infection by infectious bursal disease virus in chickens differing in their resistance to the disease.

Smith J, Sadeyen JR, Butter C, Kaiser P, Burt DW - J. Virol. (2014)

qRT-PCR analysis of 20 genes differentially expressed during IBDV infection. (A) Representative genes with inherent differences in expression between susceptible and resistant control birds. (B) Representative genes with expression changes during the host response in the susceptible line. (C) Representative genes with differential gene expression between the susceptible and resistant lines during the host response.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4325706&req=5

Figure 7: qRT-PCR analysis of 20 genes differentially expressed during IBDV infection. (A) Representative genes with inherent differences in expression between susceptible and resistant control birds. (B) Representative genes with expression changes during the host response in the susceptible line. (C) Representative genes with differential gene expression between the susceptible and resistant lines during the host response.
Mentions: Twenty-one genes were chosen for verification by qRT-PCR, including genes involved in the host response and genes differentially expressed between the susceptible and resistant lines (either inherently or during the course of infection) (Table 4). Of the 20 genes tested, differential expression in the microarray was confirmed in the qRT-PCR analysis for 17. However, the microarray results for SRFBP1, TNFRSF1B, and CARD9 were not replicated in the qRT-PCR analysis (note that the qRT-PCR assay did not work for BLB1) (Fig. 7).

Bottom Line: There is thus an urgent need to explore new control solutions, one of which would be to breed birds with greater resistance to IBD.This goal is perhaps uniquely achievable with poultry, of all farm animal species, since the genetics of 85% of the 60 billion chickens produced worldwide each year is under the control of essentially two breeding companies.In a comprehensive study, we attempt here to identify global transcriptomic differences in the target organ of the virus between chicken lines that differ in resistance and to predict candidate resistance genes.

View Article: PubMed Central - PubMed

Affiliation: The Roslin Institute and R(D)SVS, University of Edinburgh, Easter Bush, Midlothian, United Kingdom jacqueline.smith@roslin.ed.ac.uk.

Show MeSH
Related in: MedlinePlus