Analysis of the early immune response to infection by infectious bursal disease virus in chickens differing in their resistance to the disease.
Bottom Line: Vaccines are available, but field strains of the virus are of increasing virulence.This goal is perhaps uniquely achievable with poultry, of all farm animal species, since the genetics of 85% of the 60 billion chickens produced worldwide each year is under the control of essentially two breeding companies.In a comprehensive study, we attempt here to identify global transcriptomic differences in the target organ of the virus between chicken lines that differ in resistance and to predict candidate resistance genes.
Affiliation: The Roslin Institute and R(D)SVS, University of Edinburgh, Easter Bush, Midlothian, United Kingdom email@example.com.Show MeSH
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Mentions: Twenty-one genes were chosen for verification by qRT-PCR, including genes involved in the host response and genes differentially expressed between the susceptible and resistant lines (either inherently or during the course of infection) (Table 4). Of the 20 genes tested, differential expression in the microarray was confirmed in the qRT-PCR analysis for 17. However, the microarray results for SRFBP1, TNFRSF1B, and CARD9 were not replicated in the qRT-PCR analysis (note that the qRT-PCR assay did not work for BLB1) (Fig. 7).
Affiliation: The Roslin Institute and R(D)SVS, University of Edinburgh, Easter Bush, Midlothian, United Kingdom firstname.lastname@example.org.