Analysis of the early immune response to infection by infectious bursal disease virus in chickens differing in their resistance to the disease.
Bottom Line: Vaccines are available, but field strains of the virus are of increasing virulence.This goal is perhaps uniquely achievable with poultry, of all farm animal species, since the genetics of 85% of the 60 billion chickens produced worldwide each year is under the control of essentially two breeding companies.In a comprehensive study, we attempt here to identify global transcriptomic differences in the target organ of the virus between chicken lines that differ in resistance and to predict candidate resistance genes.
Affiliation: The Roslin Institute and R(D)SVS, University of Edinburgh, Easter Bush, Midlothian, United Kingdom email@example.com.Show MeSH
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Mentions: Expander was then also used to look for enrichment of GO terms associated with genes, genomic locations and TFBS within the clustered DE genes (834 upregulated and 377 downregulated). The upregulated genes were over-represented in biological processes such as “immune response,” “response to stimulus,” “cytokine activity,” and other functions associated with viral infection. Only “protein binding” was highlighted among the downregulated genes. Examination of the microarray data did not show any enrichment for a particular genomic location among the DE genes. Upregulated genes showed an enrichment of TFBS for ISRE (IFN stimulatory response element) and IRF7 (interferon regulatory factor 7). IRF7 regulates transcription of type I IFN genes and IFN-stimulated genes by binding to ISRE motifs in their promoters. Genes containing a binding site for the Drosophila transcription factor Ovo (presumably the chicken orthologue thereof) was also over-represented. No TFBS were seen to be enriched within the downregulated array genes (Fig. 5).
Affiliation: The Roslin Institute and R(D)SVS, University of Edinburgh, Easter Bush, Midlothian, United Kingdom firstname.lastname@example.org.