Analysis of the early immune response to infection by infectious bursal disease virus in chickens differing in their resistance to the disease.
Bottom Line: Vaccines are available, but field strains of the virus are of increasing virulence.This goal is perhaps uniquely achievable with poultry, of all farm animal species, since the genetics of 85% of the 60 billion chickens produced worldwide each year is under the control of essentially two breeding companies.In a comprehensive study, we attempt here to identify global transcriptomic differences in the target organ of the virus between chicken lines that differ in resistance and to predict candidate resistance genes.
Affiliation: The Roslin Institute and R(D)SVS, University of Edinburgh, Easter Bush, Midlothian, United Kingdom firstname.lastname@example.org.Show MeSH
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Mentions: The data were also analyzed using Pathway Express (23), which, based upon the KEGG pathways (24), pictorially illustrates the genes that are up- or downregulated in any given biological pathway. Figure 3 shows examples using the data from the spleen at 4 dpi. Genes involved in the extrinsic apoptosis pathway and the Toll-like receptor signaling pathway, which play integral roles during the innate immune response, are upregulated. In the bursa, genes involved in the B-cell receptor signaling and cell cycle pathways were dramatically downregulated (Fig. 4). Many of the biological responses seen in the bursa will be due to viral replication and cell damage and will not be antiviral responses per se. It must be borne in mind that these diagrams are based on the human pathways and so in some cases are not completely demonstrative of the chicken pathways, i.e., avian-specific genes are not represented. Other pathways seen to be significantly (FDR-corrected P value of <0.25) involved are shown in Table 3.
Affiliation: The Roslin Institute and R(D)SVS, University of Edinburgh, Easter Bush, Midlothian, United Kingdom email@example.com.