Limits...
Analysis of the early immune response to infection by infectious bursal disease virus in chickens differing in their resistance to the disease.

Smith J, Sadeyen JR, Butter C, Kaiser P, Burt DW - J. Virol. (2014)

Bottom Line: There is thus an urgent need to explore new control solutions, one of which would be to breed birds with greater resistance to IBD.This goal is perhaps uniquely achievable with poultry, of all farm animal species, since the genetics of 85% of the 60 billion chickens produced worldwide each year is under the control of essentially two breeding companies.In a comprehensive study, we attempt here to identify global transcriptomic differences in the target organ of the virus between chicken lines that differ in resistance and to predict candidate resistance genes.

View Article: PubMed Central - PubMed

Affiliation: The Roslin Institute and R(D)SVS, University of Edinburgh, Easter Bush, Midlothian, United Kingdom jacqueline.smith@roslin.ed.ac.uk.

Show MeSH

Related in: MedlinePlus

Pathway Express analysis of the host response to IBDV infection in the spleen. Many genes in the apoptosis (A) and TLR pathways (B) are upregulated (red). Genes in blue are downregulated.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4325706&req=5

Figure 3: Pathway Express analysis of the host response to IBDV infection in the spleen. Many genes in the apoptosis (A) and TLR pathways (B) are upregulated (red). Genes in blue are downregulated.

Mentions: The data were also analyzed using Pathway Express (23), which, based upon the KEGG pathways (24), pictorially illustrates the genes that are up- or downregulated in any given biological pathway. Figure 3 shows examples using the data from the spleen at 4 dpi. Genes involved in the extrinsic apoptosis pathway and the Toll-like receptor signaling pathway, which play integral roles during the innate immune response, are upregulated. In the bursa, genes involved in the B-cell receptor signaling and cell cycle pathways were dramatically downregulated (Fig. 4). Many of the biological responses seen in the bursa will be due to viral replication and cell damage and will not be antiviral responses per se. It must be borne in mind that these diagrams are based on the human pathways and so in some cases are not completely demonstrative of the chicken pathways, i.e., avian-specific genes are not represented. Other pathways seen to be significantly (FDR-corrected P value of <0.25) involved are shown in Table 3.


Analysis of the early immune response to infection by infectious bursal disease virus in chickens differing in their resistance to the disease.

Smith J, Sadeyen JR, Butter C, Kaiser P, Burt DW - J. Virol. (2014)

Pathway Express analysis of the host response to IBDV infection in the spleen. Many genes in the apoptosis (A) and TLR pathways (B) are upregulated (red). Genes in blue are downregulated.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4325706&req=5

Figure 3: Pathway Express analysis of the host response to IBDV infection in the spleen. Many genes in the apoptosis (A) and TLR pathways (B) are upregulated (red). Genes in blue are downregulated.
Mentions: The data were also analyzed using Pathway Express (23), which, based upon the KEGG pathways (24), pictorially illustrates the genes that are up- or downregulated in any given biological pathway. Figure 3 shows examples using the data from the spleen at 4 dpi. Genes involved in the extrinsic apoptosis pathway and the Toll-like receptor signaling pathway, which play integral roles during the innate immune response, are upregulated. In the bursa, genes involved in the B-cell receptor signaling and cell cycle pathways were dramatically downregulated (Fig. 4). Many of the biological responses seen in the bursa will be due to viral replication and cell damage and will not be antiviral responses per se. It must be borne in mind that these diagrams are based on the human pathways and so in some cases are not completely demonstrative of the chicken pathways, i.e., avian-specific genes are not represented. Other pathways seen to be significantly (FDR-corrected P value of <0.25) involved are shown in Table 3.

Bottom Line: There is thus an urgent need to explore new control solutions, one of which would be to breed birds with greater resistance to IBD.This goal is perhaps uniquely achievable with poultry, of all farm animal species, since the genetics of 85% of the 60 billion chickens produced worldwide each year is under the control of essentially two breeding companies.In a comprehensive study, we attempt here to identify global transcriptomic differences in the target organ of the virus between chicken lines that differ in resistance and to predict candidate resistance genes.

View Article: PubMed Central - PubMed

Affiliation: The Roslin Institute and R(D)SVS, University of Edinburgh, Easter Bush, Midlothian, United Kingdom jacqueline.smith@roslin.ed.ac.uk.

Show MeSH
Related in: MedlinePlus