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Astrocyte elevated gene-1 overexpression in hepatocellular carcinoma: an independent prognostic factor.

Jung HI, Ahn T, Bae SH, Chung JC, Kim H, Chin S, Jeong D, Cho HD, Lee MS, Kim HC, Kim CH, Baek MJ - Ann Surg Treat Res (2015)

Bottom Line: The association was estimated by univariate and multivariate analyses with Cox regression.The cumulative 5-year survival and recurrence rates were 89.2% and 50.0% in the low-expressing group and 24.5% and 82.4% in the high-expressing group, respectively.The results suggest that AEG-1 overexpression could serve as a valuable prognostic marker in patients with HCC.

View Article: PubMed Central - PubMed

Affiliation: Department of Surgery, Soonchunhyang University Cheonan Hospital, Cheonan, Korea.

ABSTRACT

Purpose: Astrocyte elevated gene-1 (AEG-1) plays important roles in tumorigenesis such as proliferation, invasion, metastasis, angiogenesis, and chemoresistance. We examined the expression of AEG-1 in patients with hepatocellular carcinoma (HCC).

Methods: Eighty-five samples were collected from patients with HCC who underwent surgery and were histopathologically confirmed to have HCC. Two independent pathologists, experienced in evaluating immunohistochemistry and blinded to the clinical outcomes of the patients, reviewed all samples. They determined AEG-1 expression semiquantitatively by assessing the percentage of positively stained immunoreactive cells and staining intensity. Clinicopathological data were analyzed in association with prognosis.

Results: The association was estimated by univariate and multivariate analyses with Cox regression. Tumor size (hazard ratio [HR], 2.285; 95% confidence interval [CI], 1.175-4.447; P = 0.015), microvascular invasion (HR, 6.754; 95% CI, 1.631-27.965; P = 0.008), and AEG-1 expression (HR, 4.756; 95% CI, 1.697-13.329; P = 0.003) were independent prognostic factors for overall survival. Those for disease-free survival rate were tumor size (HR, 2.245; 95% CI, 1.282-3.933; P = 0.005) and AEG-1 expression (HR, 1.916; 95% CI, 1.035-3.545; P = 0.038). The cumulative 5-year survival and recurrence rates were 89.2% and 50.0% in the low-expressing group and 24.5% and 82.4% in the high-expressing group, respectively.

Conclusion: The results suggest that AEG-1 overexpression could serve as a valuable prognostic marker in patients with HCC.

No MeSH data available.


Related in: MedlinePlus

(A) Astrocyte elevated gene-1 (AEG-1) was not expressed in the cytoplasm of normal hepatocytes. (B) AEG-1 was mildly expressed in tumor cells of Edmondson and Steiner (ES) grade I hepatocellular carcinoma (HCC) (score, 1). (C) AEG-1 was moderately expressed in the cytoplasm of ES grade II HCC (score, 2). (D) AEG-1 was strongly expressed in the cytoplasm of ES grade III HCC (score, 3). (E) AEG-1 was strongly expressed in the cytoplasm of ES grade IV HCC (score, 3). (F) AEG-1 was strongly expressed in the cytoplasm of highly infiltrative HCC (score, 3). Scale: A-D, 50 µm; E-F, 100 µm.
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Figure 1: (A) Astrocyte elevated gene-1 (AEG-1) was not expressed in the cytoplasm of normal hepatocytes. (B) AEG-1 was mildly expressed in tumor cells of Edmondson and Steiner (ES) grade I hepatocellular carcinoma (HCC) (score, 1). (C) AEG-1 was moderately expressed in the cytoplasm of ES grade II HCC (score, 2). (D) AEG-1 was strongly expressed in the cytoplasm of ES grade III HCC (score, 3). (E) AEG-1 was strongly expressed in the cytoplasm of ES grade IV HCC (score, 3). (F) AEG-1 was strongly expressed in the cytoplasm of highly infiltrative HCC (score, 3). Scale: A-D, 50 µm; E-F, 100 µm.

Mentions: We examined IHC staining of paraffin-embedded, archived HCC tissues from normal hepatocytes to highly infiltrated HCC to determine whether AEG-1 overexpression was related to the ES grading system. Only negative AEG-1 expression was observed in normal hepatocytes (Fig. 1A). The frequency and intensity of AEG-1 expression increased gradually from ES grades I to IV and were highest in highly infiltrative HCC (Fig. 1B-F).


Astrocyte elevated gene-1 overexpression in hepatocellular carcinoma: an independent prognostic factor.

Jung HI, Ahn T, Bae SH, Chung JC, Kim H, Chin S, Jeong D, Cho HD, Lee MS, Kim HC, Kim CH, Baek MJ - Ann Surg Treat Res (2015)

(A) Astrocyte elevated gene-1 (AEG-1) was not expressed in the cytoplasm of normal hepatocytes. (B) AEG-1 was mildly expressed in tumor cells of Edmondson and Steiner (ES) grade I hepatocellular carcinoma (HCC) (score, 1). (C) AEG-1 was moderately expressed in the cytoplasm of ES grade II HCC (score, 2). (D) AEG-1 was strongly expressed in the cytoplasm of ES grade III HCC (score, 3). (E) AEG-1 was strongly expressed in the cytoplasm of ES grade IV HCC (score, 3). (F) AEG-1 was strongly expressed in the cytoplasm of highly infiltrative HCC (score, 3). Scale: A-D, 50 µm; E-F, 100 µm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4325651&req=5

Figure 1: (A) Astrocyte elevated gene-1 (AEG-1) was not expressed in the cytoplasm of normal hepatocytes. (B) AEG-1 was mildly expressed in tumor cells of Edmondson and Steiner (ES) grade I hepatocellular carcinoma (HCC) (score, 1). (C) AEG-1 was moderately expressed in the cytoplasm of ES grade II HCC (score, 2). (D) AEG-1 was strongly expressed in the cytoplasm of ES grade III HCC (score, 3). (E) AEG-1 was strongly expressed in the cytoplasm of ES grade IV HCC (score, 3). (F) AEG-1 was strongly expressed in the cytoplasm of highly infiltrative HCC (score, 3). Scale: A-D, 50 µm; E-F, 100 µm.
Mentions: We examined IHC staining of paraffin-embedded, archived HCC tissues from normal hepatocytes to highly infiltrated HCC to determine whether AEG-1 overexpression was related to the ES grading system. Only negative AEG-1 expression was observed in normal hepatocytes (Fig. 1A). The frequency and intensity of AEG-1 expression increased gradually from ES grades I to IV and were highest in highly infiltrative HCC (Fig. 1B-F).

Bottom Line: The association was estimated by univariate and multivariate analyses with Cox regression.The cumulative 5-year survival and recurrence rates were 89.2% and 50.0% in the low-expressing group and 24.5% and 82.4% in the high-expressing group, respectively.The results suggest that AEG-1 overexpression could serve as a valuable prognostic marker in patients with HCC.

View Article: PubMed Central - PubMed

Affiliation: Department of Surgery, Soonchunhyang University Cheonan Hospital, Cheonan, Korea.

ABSTRACT

Purpose: Astrocyte elevated gene-1 (AEG-1) plays important roles in tumorigenesis such as proliferation, invasion, metastasis, angiogenesis, and chemoresistance. We examined the expression of AEG-1 in patients with hepatocellular carcinoma (HCC).

Methods: Eighty-five samples were collected from patients with HCC who underwent surgery and were histopathologically confirmed to have HCC. Two independent pathologists, experienced in evaluating immunohistochemistry and blinded to the clinical outcomes of the patients, reviewed all samples. They determined AEG-1 expression semiquantitatively by assessing the percentage of positively stained immunoreactive cells and staining intensity. Clinicopathological data were analyzed in association with prognosis.

Results: The association was estimated by univariate and multivariate analyses with Cox regression. Tumor size (hazard ratio [HR], 2.285; 95% confidence interval [CI], 1.175-4.447; P = 0.015), microvascular invasion (HR, 6.754; 95% CI, 1.631-27.965; P = 0.008), and AEG-1 expression (HR, 4.756; 95% CI, 1.697-13.329; P = 0.003) were independent prognostic factors for overall survival. Those for disease-free survival rate were tumor size (HR, 2.245; 95% CI, 1.282-3.933; P = 0.005) and AEG-1 expression (HR, 1.916; 95% CI, 1.035-3.545; P = 0.038). The cumulative 5-year survival and recurrence rates were 89.2% and 50.0% in the low-expressing group and 24.5% and 82.4% in the high-expressing group, respectively.

Conclusion: The results suggest that AEG-1 overexpression could serve as a valuable prognostic marker in patients with HCC.

No MeSH data available.


Related in: MedlinePlus