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In vivo Image of Cerebral Amyloid Angiopathy in an Alzheimer's Disease Mouse Model.

Kim J, Jeong Y - J Stroke (2015)

View Article: PubMed Central - PubMed

Affiliation: Department of Bio and Brain Engineering, KAIST, Daejeon, Korea.

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Dear Sir: We would like to share an in vivo image of cerebral amyloid angiopathy (CAA) in an Alzheimer's disease (AD) mouse model... In AD, insoluble amyloid beta (Aβ) protein forms plaques in the parenchyma and also accumulates along the vessel walls... Images were taken using a 20×objective lens (NA 1.0; Carl Zeiss) with a frame rate of 0.5-1 Hz... All images were obtained using the pulsed laser at an excitation wavelength of 800 nm... We discriminated between arterioles and venules based on the direction of red blood cell flow and the morphology of the vessels... As shown in the Figure 1 (and supplementary movie clip 1 for z-stack image), Aβ deposits were wrapped around the vessel wall in patches... Most plaques did not form complete rings forms at this stage... Seven months is still relatively young, and older mice would have dense Aβ deposits and complete ring forms of CAA... Interestingly, CAA was not observed in either the veins or the dura vessels... This finding supports the concept that CAA is formed by failure of Aβ elimination along the peri-arterial wall, but not along the peri-venous wall.

No MeSH data available.


Related in: MedlinePlus

Plaque and cerebral vessel imaging in an APPswe/PS1ΔE9 transgenic mouse (7-month-old; male). Methoxy-X04 (5 mg/kg) was injected intraperitoneally 1 day before the imaging session for plaque imaging. Texas Red-dextran (70 kDa) was injected intravenously on the day of imaging for vessel imaging. Imaging using two-photon laser scanning microscopy was performed up to a depth of 100 µm from the exposed dura. Amyloid aggregates (blue) are deposited on the cerebral arteriole wall (cerebral amyloid angiopathy; CAA) as well as in the brain parenchyma (amyloid plaques). CAA is not observed around the veins or dura vessels. (Red, cerebral blood vessel stained with Texas Red-dextran; a, arteriole; d, dura vessel; v, vein; arrowheads, amyloid plaques; scale bar=100 µm)
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Figure 1: Plaque and cerebral vessel imaging in an APPswe/PS1ΔE9 transgenic mouse (7-month-old; male). Methoxy-X04 (5 mg/kg) was injected intraperitoneally 1 day before the imaging session for plaque imaging. Texas Red-dextran (70 kDa) was injected intravenously on the day of imaging for vessel imaging. Imaging using two-photon laser scanning microscopy was performed up to a depth of 100 µm from the exposed dura. Amyloid aggregates (blue) are deposited on the cerebral arteriole wall (cerebral amyloid angiopathy; CAA) as well as in the brain parenchyma (amyloid plaques). CAA is not observed around the veins or dura vessels. (Red, cerebral blood vessel stained with Texas Red-dextran; a, arteriole; d, dura vessel; v, vein; arrowheads, amyloid plaques; scale bar=100 µm)

Mentions: As shown in the Figure 1 (and supplementary movie clip 1 for z-stack image), Aβ deposits were wrapped around the vessel wall in patches. Most plaques did not form complete rings forms at this stage. Seven months is still relatively young, and older mice would have dense Aβ deposits and complete ring forms of CAA. Interestingly, CAA was not observed in either the veins or the dura vessels.1 This finding supports the concept that CAA is formed by failure of Aβ elimination along the peri-arterial wall, but not along the peri-venous wall.6


In vivo Image of Cerebral Amyloid Angiopathy in an Alzheimer's Disease Mouse Model.

Kim J, Jeong Y - J Stroke (2015)

Plaque and cerebral vessel imaging in an APPswe/PS1ΔE9 transgenic mouse (7-month-old; male). Methoxy-X04 (5 mg/kg) was injected intraperitoneally 1 day before the imaging session for plaque imaging. Texas Red-dextran (70 kDa) was injected intravenously on the day of imaging for vessel imaging. Imaging using two-photon laser scanning microscopy was performed up to a depth of 100 µm from the exposed dura. Amyloid aggregates (blue) are deposited on the cerebral arteriole wall (cerebral amyloid angiopathy; CAA) as well as in the brain parenchyma (amyloid plaques). CAA is not observed around the veins or dura vessels. (Red, cerebral blood vessel stained with Texas Red-dextran; a, arteriole; d, dura vessel; v, vein; arrowheads, amyloid plaques; scale bar=100 µm)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4325644&req=5

Figure 1: Plaque and cerebral vessel imaging in an APPswe/PS1ΔE9 transgenic mouse (7-month-old; male). Methoxy-X04 (5 mg/kg) was injected intraperitoneally 1 day before the imaging session for plaque imaging. Texas Red-dextran (70 kDa) was injected intravenously on the day of imaging for vessel imaging. Imaging using two-photon laser scanning microscopy was performed up to a depth of 100 µm from the exposed dura. Amyloid aggregates (blue) are deposited on the cerebral arteriole wall (cerebral amyloid angiopathy; CAA) as well as in the brain parenchyma (amyloid plaques). CAA is not observed around the veins or dura vessels. (Red, cerebral blood vessel stained with Texas Red-dextran; a, arteriole; d, dura vessel; v, vein; arrowheads, amyloid plaques; scale bar=100 µm)
Mentions: As shown in the Figure 1 (and supplementary movie clip 1 for z-stack image), Aβ deposits were wrapped around the vessel wall in patches. Most plaques did not form complete rings forms at this stage. Seven months is still relatively young, and older mice would have dense Aβ deposits and complete ring forms of CAA. Interestingly, CAA was not observed in either the veins or the dura vessels.1 This finding supports the concept that CAA is formed by failure of Aβ elimination along the peri-arterial wall, but not along the peri-venous wall.6

View Article: PubMed Central - PubMed

Affiliation: Department of Bio and Brain Engineering, KAIST, Daejeon, Korea.

AUTOMATICALLY GENERATED EXCERPT
Please rate it.

Dear Sir: We would like to share an in vivo image of cerebral amyloid angiopathy (CAA) in an Alzheimer's disease (AD) mouse model... In AD, insoluble amyloid beta (Aβ) protein forms plaques in the parenchyma and also accumulates along the vessel walls... Images were taken using a 20×objective lens (NA 1.0; Carl Zeiss) with a frame rate of 0.5-1 Hz... All images were obtained using the pulsed laser at an excitation wavelength of 800 nm... We discriminated between arterioles and venules based on the direction of red blood cell flow and the morphology of the vessels... As shown in the Figure 1 (and supplementary movie clip 1 for z-stack image), Aβ deposits were wrapped around the vessel wall in patches... Most plaques did not form complete rings forms at this stage... Seven months is still relatively young, and older mice would have dense Aβ deposits and complete ring forms of CAA... Interestingly, CAA was not observed in either the veins or the dura vessels... This finding supports the concept that CAA is formed by failure of Aβ elimination along the peri-arterial wall, but not along the peri-venous wall.

No MeSH data available.


Related in: MedlinePlus