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Effect of mouse antisera targeting the Phlebotomus papatasi midgut chitinase PpChit1 on sandfly physiology and fitness.

Robles-Murguia M, Bloedow N, Murray L, Ramalho-Ortigão M - Mem. Inst. Oswaldo Cruz (2014)

Bottom Line: We previously characterised PpChit1, a midgut-specific chitinase secreted in Phlebotomus papatasi (PPIS) that is involved in the maturation of the PM and showed that antibodies against PpChit1 reduce the chitinolytic activity in the midgut of several sandfly species.Anti-PpChit1 also had a negative effect on overall ability of flies to lay eggs, as several gravid females from all three species were unable to lay any eggs despite having lived longer than control flies.Whereas the longer survival might be associated with improved haeme scavenging ability by the PM, the inability of females to lay eggs is possibly linked to changes in PM permeability affecting nutrient absorption.

View Article: PubMed Central - PubMed

Affiliation: Department of Entomology, Kansas State University, Manhattan, KS, USA.

ABSTRACT
In sandflies, the absence of the peritrophic matrix (PM) affects the rate of blood digestion. Also, the kinetics of PM secretion varies according to species. We previously characterised PpChit1, a midgut-specific chitinase secreted in Phlebotomus papatasi (PPIS) that is involved in the maturation of the PM and showed that antibodies against PpChit1 reduce the chitinolytic activity in the midgut of several sandfly species. Here, sandflies were fed on red blood cells reconstituted with naïve or anti-PpChit1 sera and assessed for fitness parameters that included blood digestion, oviposition onset, number of eggs laid, egg bouts, average number of eggs per bout and survival. In PPIS, anti-PpChit1 led to a one-day delay in the onset of egg laying, with flies surviving three days longer compared to the control group. Anti-PpChit1 also had a negative effect on overall ability of flies to lay eggs, as several gravid females from all three species were unable to lay any eggs despite having lived longer than control flies. Whereas the longer survival might be associated with improved haeme scavenging ability by the PM, the inability of females to lay eggs is possibly linked to changes in PM permeability affecting nutrient absorption.

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Related in: MedlinePlus

Sandfly midgut digestion process over time. Midguts of sandflies fed onred blood cells reconstituted in naïve (A) or anti-PpChit1 (B) sera anddissected at 24, 30, 36 and 48 h post blood ingestion. Midguts are displayedwith their anterior end (or thoracic portion) to the left and the posteriorend to the right.
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f20: Sandfly midgut digestion process over time. Midguts of sandflies fed onred blood cells reconstituted in naïve (A) or anti-PpChit1 (B) sera anddissected at 24, 30, 36 and 48 h post blood ingestion. Midguts are displayedwith their anterior end (or thoracic portion) to the left and the posteriorend to the right.


Effect of mouse antisera targeting the Phlebotomus papatasi midgut chitinase PpChit1 on sandfly physiology and fitness.

Robles-Murguia M, Bloedow N, Murray L, Ramalho-Ortigão M - Mem. Inst. Oswaldo Cruz (2014)

Sandfly midgut digestion process over time. Midguts of sandflies fed onred blood cells reconstituted in naïve (A) or anti-PpChit1 (B) sera anddissected at 24, 30, 36 and 48 h post blood ingestion. Midguts are displayedwith their anterior end (or thoracic portion) to the left and the posteriorend to the right.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4325622&req=5

f20: Sandfly midgut digestion process over time. Midguts of sandflies fed onred blood cells reconstituted in naïve (A) or anti-PpChit1 (B) sera anddissected at 24, 30, 36 and 48 h post blood ingestion. Midguts are displayedwith their anterior end (or thoracic portion) to the left and the posteriorend to the right.
Bottom Line: We previously characterised PpChit1, a midgut-specific chitinase secreted in Phlebotomus papatasi (PPIS) that is involved in the maturation of the PM and showed that antibodies against PpChit1 reduce the chitinolytic activity in the midgut of several sandfly species.Anti-PpChit1 also had a negative effect on overall ability of flies to lay eggs, as several gravid females from all three species were unable to lay any eggs despite having lived longer than control flies.Whereas the longer survival might be associated with improved haeme scavenging ability by the PM, the inability of females to lay eggs is possibly linked to changes in PM permeability affecting nutrient absorption.

View Article: PubMed Central - PubMed

Affiliation: Department of Entomology, Kansas State University, Manhattan, KS, USA.

ABSTRACT
In sandflies, the absence of the peritrophic matrix (PM) affects the rate of blood digestion. Also, the kinetics of PM secretion varies according to species. We previously characterised PpChit1, a midgut-specific chitinase secreted in Phlebotomus papatasi (PPIS) that is involved in the maturation of the PM and showed that antibodies against PpChit1 reduce the chitinolytic activity in the midgut of several sandfly species. Here, sandflies were fed on red blood cells reconstituted with naïve or anti-PpChit1 sera and assessed for fitness parameters that included blood digestion, oviposition onset, number of eggs laid, egg bouts, average number of eggs per bout and survival. In PPIS, anti-PpChit1 led to a one-day delay in the onset of egg laying, with flies surviving three days longer compared to the control group. Anti-PpChit1 also had a negative effect on overall ability of flies to lay eggs, as several gravid females from all three species were unable to lay any eggs despite having lived longer than control flies. Whereas the longer survival might be associated with improved haeme scavenging ability by the PM, the inability of females to lay eggs is possibly linked to changes in PM permeability affecting nutrient absorption.

Show MeSH
Related in: MedlinePlus