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Brazilian Angiostrongylus cantonensis haplotypes, ac8 and ac9, have two different biological and morphological profiles.

Monte TC, Gentile R, Garcia J, Mota E, Santos JN, Maldonado Júnior A - Mem. Inst. Oswaldo Cruz (2014)

Bottom Line: A significantly larger number of L1 larvae eliminated in the faeces of rodents at the beginning of the patent period was observed for ac9 haplotype and compared to the total of L1 larvae eliminated, there was a significant difference between the two haplotypes.The ac9 haplotype showed a significant difference in the proportion of female and male specimens (0.6:1), but the same was not observed for ac8 (1.2:1).The biological, morphological and morphometric variations observed between the two haplotypes agree with the observed variation at the molecular level using the cytochrome oxidase subunit I marker and reinforce the possible influence of geographical isolation on the development of these haplotypes.

View Article: PubMed Central - PubMed

Affiliation: Laboratório de Biologia e Parasitologia de Mamíferos Silvestres Reservatórios, Instituto Oswaldo Cruz-Fiocruz, Rio de Janeiro, RJ, Brasil.

ABSTRACT
Angiostrongylus cantonensis is the etiologic agent of eosinophilic meningoencephalitis in humans. Cases have been recorded in many parts of the world, including Brazil. The aim of this study was to compare the differences in the biology and morphology of two different Brazilian haplotypes of A. cantonensis: ac8 and ac9. A significantly larger number of L1 larvae eliminated in the faeces of rodents at the beginning of the patent period was observed for ac9 haplotype and compared to the total of L1 larvae eliminated, there was a significant difference between the two haplotypes. The ac9 haplotype showed a significant difference in the proportion of female and male specimens (0.6:1), but the same was not observed for ac8 (1.2:1). The morphometric analysis showed that male and female specimens isolated from ac8 haplotype were significantly larger with respect to body length, oesophagus length, spicule length (male) and distance from the anus to the rear end (female) compared to specimens from ac9. The morphological analysis by light microscopy showed little variation in the level of bifurcations at the lateral rays in the right lobe of the copulatory bursa between the two haplotypes. The biological, morphological and morphometric variations observed between the two haplotypes agree with the observed variation at the molecular level using the cytochrome oxidase subunit I marker and reinforce the possible influence of geographical isolation on the development of these haplotypes.

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: light microscopy of Angiostrongylus cantonensis. A: ac8haplotype; B: ac9 haplotype, dorsal view of caudal bursa; C: ac8 haplotype; D:ac9 haplotype, right lobe of caudal bursa; E: ac8 haplotype; F: ac9 haplotype,left lobe of caudal bursa. Arrows indicate the differences in the level ofbifurcations at the lateral rays of caudal bursa. Bar = 50 µm.
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f04: : light microscopy of Angiostrongylus cantonensis. A: ac8haplotype; B: ac9 haplotype, dorsal view of caudal bursa; C: ac8 haplotype; D:ac9 haplotype, right lobe of caudal bursa; E: ac8 haplotype; F: ac9 haplotype,left lobe of caudal bursa. Arrows indicate the differences in the level ofbifurcations at the lateral rays of caudal bursa. Bar = 50 µm.

Mentions: Morphological analysis of adult helminths - The morphological analysisby light microscopy showed a slight variation at the level of bifurcations that unitethe lateral rays of the copulatory bursa of male specimens. There were differences atthe level of bifurcations of lateral rays (4, 5 and 6) in the right lobe of ac8haplotype, while no differences were observed at the level of bifurcations of the samelateral rays for ac9. Variations were also observed in the ventral rays and dorsal raysbetween the two haplotypes. Specimens of ac8 showed shorter and thinner ventral rayswhen compared with ac9 specimens and the dorsal rays of ac8 specimens were moreprominent (Figs 3, 4).


Brazilian Angiostrongylus cantonensis haplotypes, ac8 and ac9, have two different biological and morphological profiles.

Monte TC, Gentile R, Garcia J, Mota E, Santos JN, Maldonado Júnior A - Mem. Inst. Oswaldo Cruz (2014)

: light microscopy of Angiostrongylus cantonensis. A: ac8haplotype; B: ac9 haplotype, dorsal view of caudal bursa; C: ac8 haplotype; D:ac9 haplotype, right lobe of caudal bursa; E: ac8 haplotype; F: ac9 haplotype,left lobe of caudal bursa. Arrows indicate the differences in the level ofbifurcations at the lateral rays of caudal bursa. Bar = 50 µm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4325621&req=5

f04: : light microscopy of Angiostrongylus cantonensis. A: ac8haplotype; B: ac9 haplotype, dorsal view of caudal bursa; C: ac8 haplotype; D:ac9 haplotype, right lobe of caudal bursa; E: ac8 haplotype; F: ac9 haplotype,left lobe of caudal bursa. Arrows indicate the differences in the level ofbifurcations at the lateral rays of caudal bursa. Bar = 50 µm.
Mentions: Morphological analysis of adult helminths - The morphological analysisby light microscopy showed a slight variation at the level of bifurcations that unitethe lateral rays of the copulatory bursa of male specimens. There were differences atthe level of bifurcations of lateral rays (4, 5 and 6) in the right lobe of ac8haplotype, while no differences were observed at the level of bifurcations of the samelateral rays for ac9. Variations were also observed in the ventral rays and dorsal raysbetween the two haplotypes. Specimens of ac8 showed shorter and thinner ventral rayswhen compared with ac9 specimens and the dorsal rays of ac8 specimens were moreprominent (Figs 3, 4).

Bottom Line: A significantly larger number of L1 larvae eliminated in the faeces of rodents at the beginning of the patent period was observed for ac9 haplotype and compared to the total of L1 larvae eliminated, there was a significant difference between the two haplotypes.The ac9 haplotype showed a significant difference in the proportion of female and male specimens (0.6:1), but the same was not observed for ac8 (1.2:1).The biological, morphological and morphometric variations observed between the two haplotypes agree with the observed variation at the molecular level using the cytochrome oxidase subunit I marker and reinforce the possible influence of geographical isolation on the development of these haplotypes.

View Article: PubMed Central - PubMed

Affiliation: Laboratório de Biologia e Parasitologia de Mamíferos Silvestres Reservatórios, Instituto Oswaldo Cruz-Fiocruz, Rio de Janeiro, RJ, Brasil.

ABSTRACT
Angiostrongylus cantonensis is the etiologic agent of eosinophilic meningoencephalitis in humans. Cases have been recorded in many parts of the world, including Brazil. The aim of this study was to compare the differences in the biology and morphology of two different Brazilian haplotypes of A. cantonensis: ac8 and ac9. A significantly larger number of L1 larvae eliminated in the faeces of rodents at the beginning of the patent period was observed for ac9 haplotype and compared to the total of L1 larvae eliminated, there was a significant difference between the two haplotypes. The ac9 haplotype showed a significant difference in the proportion of female and male specimens (0.6:1), but the same was not observed for ac8 (1.2:1). The morphometric analysis showed that male and female specimens isolated from ac8 haplotype were significantly larger with respect to body length, oesophagus length, spicule length (male) and distance from the anus to the rear end (female) compared to specimens from ac9. The morphological analysis by light microscopy showed little variation in the level of bifurcations at the lateral rays in the right lobe of the copulatory bursa between the two haplotypes. The biological, morphological and morphometric variations observed between the two haplotypes agree with the observed variation at the molecular level using the cytochrome oxidase subunit I marker and reinforce the possible influence of geographical isolation on the development of these haplotypes.

Show MeSH
Related in: MedlinePlus