Limits...
Brazilian Angiostrongylus cantonensis haplotypes, ac8 and ac9, have two different biological and morphological profiles.

Monte TC, Gentile R, Garcia J, Mota E, Santos JN, Maldonado Júnior A - Mem. Inst. Oswaldo Cruz (2014)

Bottom Line: A significantly larger number of L1 larvae eliminated in the faeces of rodents at the beginning of the patent period was observed for ac9 haplotype and compared to the total of L1 larvae eliminated, there was a significant difference between the two haplotypes.The ac9 haplotype showed a significant difference in the proportion of female and male specimens (0.6:1), but the same was not observed for ac8 (1.2:1).The biological, morphological and morphometric variations observed between the two haplotypes agree with the observed variation at the molecular level using the cytochrome oxidase subunit I marker and reinforce the possible influence of geographical isolation on the development of these haplotypes.

View Article: PubMed Central - PubMed

Affiliation: Laboratório de Biologia e Parasitologia de Mamíferos Silvestres Reservatórios, Instituto Oswaldo Cruz-Fiocruz, Rio de Janeiro, RJ, Brasil.

ABSTRACT
Angiostrongylus cantonensis is the etiologic agent of eosinophilic meningoencephalitis in humans. Cases have been recorded in many parts of the world, including Brazil. The aim of this study was to compare the differences in the biology and morphology of two different Brazilian haplotypes of A. cantonensis: ac8 and ac9. A significantly larger number of L1 larvae eliminated in the faeces of rodents at the beginning of the patent period was observed for ac9 haplotype and compared to the total of L1 larvae eliminated, there was a significant difference between the two haplotypes. The ac9 haplotype showed a significant difference in the proportion of female and male specimens (0.6:1), but the same was not observed for ac8 (1.2:1). The morphometric analysis showed that male and female specimens isolated from ac8 haplotype were significantly larger with respect to body length, oesophagus length, spicule length (male) and distance from the anus to the rear end (female) compared to specimens from ac9. The morphological analysis by light microscopy showed little variation in the level of bifurcations at the lateral rays in the right lobe of the copulatory bursa between the two haplotypes. The biological, morphological and morphometric variations observed between the two haplotypes agree with the observed variation at the molecular level using the cytochrome oxidase subunit I marker and reinforce the possible influence of geographical isolation on the development of these haplotypes.

Show MeSH

Related in: MedlinePlus

: intensity of infection for ac8 and ac9 haplotypes given by the medianfrom the weekly mean of L1 larvae eliminated per gram of faeces.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4325621&req=5

f02: : intensity of infection for ac8 and ac9 haplotypes given by the medianfrom the weekly mean of L1 larvae eliminated per gram of faeces.

Mentions: A progressive increase was observed in the number of L1 larvae eliminated in the faecesfrom the seventh to ninth and 10th week of infection for ac9 and ac8 haplotypes,respectively. In the following weeks, fluctuation was observed in the L1 larvaeelimination for ac9, with a decrease after the 11th week. For ac8 haplotype, there was aslight continuous increase in larvae elimination until the end of the experiment, at the12th week (Fig. 2). The difference in the L1larvae elimination of each haplotype between weeks was also statistically significantwith progression of the infection (haplotype ac9: H = 15.73; p = 0.0076; haplotype ac8:F = 14.08; df = 5; p = 1.772E-06). The comparison of total L1 larvae eliminated showed asignificant difference between the two haplotypes (t = -2.341; p = 0.047). We alsoobserved a significant difference in L1 larvae elimination between the haplotypes whencompared within weeks, which showed different profiles (F = 8.66; gl = 5.48; p< 0.0001) (Fig. 2, Table I).


Brazilian Angiostrongylus cantonensis haplotypes, ac8 and ac9, have two different biological and morphological profiles.

Monte TC, Gentile R, Garcia J, Mota E, Santos JN, Maldonado Júnior A - Mem. Inst. Oswaldo Cruz (2014)

: intensity of infection for ac8 and ac9 haplotypes given by the medianfrom the weekly mean of L1 larvae eliminated per gram of faeces.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4325621&req=5

f02: : intensity of infection for ac8 and ac9 haplotypes given by the medianfrom the weekly mean of L1 larvae eliminated per gram of faeces.
Mentions: A progressive increase was observed in the number of L1 larvae eliminated in the faecesfrom the seventh to ninth and 10th week of infection for ac9 and ac8 haplotypes,respectively. In the following weeks, fluctuation was observed in the L1 larvaeelimination for ac9, with a decrease after the 11th week. For ac8 haplotype, there was aslight continuous increase in larvae elimination until the end of the experiment, at the12th week (Fig. 2). The difference in the L1larvae elimination of each haplotype between weeks was also statistically significantwith progression of the infection (haplotype ac9: H = 15.73; p = 0.0076; haplotype ac8:F = 14.08; df = 5; p = 1.772E-06). The comparison of total L1 larvae eliminated showed asignificant difference between the two haplotypes (t = -2.341; p = 0.047). We alsoobserved a significant difference in L1 larvae elimination between the haplotypes whencompared within weeks, which showed different profiles (F = 8.66; gl = 5.48; p< 0.0001) (Fig. 2, Table I).

Bottom Line: A significantly larger number of L1 larvae eliminated in the faeces of rodents at the beginning of the patent period was observed for ac9 haplotype and compared to the total of L1 larvae eliminated, there was a significant difference between the two haplotypes.The ac9 haplotype showed a significant difference in the proportion of female and male specimens (0.6:1), but the same was not observed for ac8 (1.2:1).The biological, morphological and morphometric variations observed between the two haplotypes agree with the observed variation at the molecular level using the cytochrome oxidase subunit I marker and reinforce the possible influence of geographical isolation on the development of these haplotypes.

View Article: PubMed Central - PubMed

Affiliation: Laboratório de Biologia e Parasitologia de Mamíferos Silvestres Reservatórios, Instituto Oswaldo Cruz-Fiocruz, Rio de Janeiro, RJ, Brasil.

ABSTRACT
Angiostrongylus cantonensis is the etiologic agent of eosinophilic meningoencephalitis in humans. Cases have been recorded in many parts of the world, including Brazil. The aim of this study was to compare the differences in the biology and morphology of two different Brazilian haplotypes of A. cantonensis: ac8 and ac9. A significantly larger number of L1 larvae eliminated in the faeces of rodents at the beginning of the patent period was observed for ac9 haplotype and compared to the total of L1 larvae eliminated, there was a significant difference between the two haplotypes. The ac9 haplotype showed a significant difference in the proportion of female and male specimens (0.6:1), but the same was not observed for ac8 (1.2:1). The morphometric analysis showed that male and female specimens isolated from ac8 haplotype were significantly larger with respect to body length, oesophagus length, spicule length (male) and distance from the anus to the rear end (female) compared to specimens from ac9. The morphological analysis by light microscopy showed little variation in the level of bifurcations at the lateral rays in the right lobe of the copulatory bursa between the two haplotypes. The biological, morphological and morphometric variations observed between the two haplotypes agree with the observed variation at the molecular level using the cytochrome oxidase subunit I marker and reinforce the possible influence of geographical isolation on the development of these haplotypes.

Show MeSH
Related in: MedlinePlus