Limits...
In vitro activity of the hydroethanolic extract and biflavonoids isolated from Selaginella sellowii on Leishmania (Leishmania) amazonensis.

Rizk YS, Fischer A, Cunha Mde C, Rodrigues PO, Marques MC, Matos Mde F, Kadri MC, Carollo CA, Arruda CC - Mem. Inst. Oswaldo Cruz (2014)

Bottom Line: SSHE was highly active against intracellular amastigotes [the half maximum inhibitory concentration (IC50) = 20.2 µg/mL].The production of nitric oxide (NO) was lower in cells treated with amentoflavone (suggesting that NO does not contribute to the leishmanicidal mechanism in this case), while NO release was higher after treatment with robustaflavone.S. sellowii may be a potential source of biflavonoids that could provide promising compounds for the treatment of cutaneous leishmaniasis.

View Article: PubMed Central - PubMed

Affiliation: Laboratório de Parasitologia Humana, Centro de Ciências Biológicas e da Saúde, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brasil.

ABSTRACT
This study is the first phytochemical investigation of Selaginella sellowii and demonstrates the antileishmanial activity of the hydroethanolic extract from this plant (SSHE), as well as of the biflavonoids amentoflavone and robustaflavone, isolated from this species. The effects of these substances were evaluated on intracellular amastigotes of Leishmania (Leishmania) amazonensis, an aetiological agent of American cutaneous leishmaniasis. SSHE was highly active against intracellular amastigotes [the half maximum inhibitory concentration (IC50) = 20.2 µg/mL]. Fractionation of the extract led to the isolation of the two bioflavonoids with the highest activity: amentoflavone, which was about 200 times more active (IC50 = 0.1 μg/mL) and less cytotoxic than SSHE (IC50 = 2.2 and 3 μg/mL, respectively on NIH/3T3 and J774.A1 cells), with a high selectivity index (SI) (22 and 30), robustaflavone, which was also active against L. amazonensis (IC50 = 2.8 µg/mL), but more cytotoxic, with IC50 = 25.5 µg/mL (SI = 9.1) on NIH/3T3 cells and IC50 = 3.1 µg/mL (SI = 1.1) on J774.A1 cells. The production of nitric oxide (NO) was lower in cells treated with amentoflavone (suggesting that NO does not contribute to the leishmanicidal mechanism in this case), while NO release was higher after treatment with robustaflavone. S. sellowii may be a potential source of biflavonoids that could provide promising compounds for the treatment of cutaneous leishmaniasis.

Show MeSH

Related in: MedlinePlus

: chromatographic profile at 270 nm and ultraviolet absorption spectra(240-400 nm) of the hydroethanolic extract from Selaginellasellowii.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4325620&req=5

f03: : chromatographic profile at 270 nm and ultraviolet absorption spectra(240-400 nm) of the hydroethanolic extract from Selaginellasellowii.

Mentions: The analysis of ultraviolet spectra of SSHE compounds revealed the presence ofphenylpropanoids (Silverstein et al. 2007) andbiflavonoids (Mabry et al. 1970) (Fig. 3). The fractionation led to the isolation oftwo biflavonoids described in the genus Selaginella: amentoflavone(Ma et al. 2001, Zhang et al. 2011) and robustaflavone (Lin et al. 2000, Zhang et al.2011).


In vitro activity of the hydroethanolic extract and biflavonoids isolated from Selaginella sellowii on Leishmania (Leishmania) amazonensis.

Rizk YS, Fischer A, Cunha Mde C, Rodrigues PO, Marques MC, Matos Mde F, Kadri MC, Carollo CA, Arruda CC - Mem. Inst. Oswaldo Cruz (2014)

: chromatographic profile at 270 nm and ultraviolet absorption spectra(240-400 nm) of the hydroethanolic extract from Selaginellasellowii.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4325620&req=5

f03: : chromatographic profile at 270 nm and ultraviolet absorption spectra(240-400 nm) of the hydroethanolic extract from Selaginellasellowii.
Mentions: The analysis of ultraviolet spectra of SSHE compounds revealed the presence ofphenylpropanoids (Silverstein et al. 2007) andbiflavonoids (Mabry et al. 1970) (Fig. 3). The fractionation led to the isolation oftwo biflavonoids described in the genus Selaginella: amentoflavone(Ma et al. 2001, Zhang et al. 2011) and robustaflavone (Lin et al. 2000, Zhang et al.2011).

Bottom Line: SSHE was highly active against intracellular amastigotes [the half maximum inhibitory concentration (IC50) = 20.2 µg/mL].The production of nitric oxide (NO) was lower in cells treated with amentoflavone (suggesting that NO does not contribute to the leishmanicidal mechanism in this case), while NO release was higher after treatment with robustaflavone.S. sellowii may be a potential source of biflavonoids that could provide promising compounds for the treatment of cutaneous leishmaniasis.

View Article: PubMed Central - PubMed

Affiliation: Laboratório de Parasitologia Humana, Centro de Ciências Biológicas e da Saúde, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brasil.

ABSTRACT
This study is the first phytochemical investigation of Selaginella sellowii and demonstrates the antileishmanial activity of the hydroethanolic extract from this plant (SSHE), as well as of the biflavonoids amentoflavone and robustaflavone, isolated from this species. The effects of these substances were evaluated on intracellular amastigotes of Leishmania (Leishmania) amazonensis, an aetiological agent of American cutaneous leishmaniasis. SSHE was highly active against intracellular amastigotes [the half maximum inhibitory concentration (IC50) = 20.2 µg/mL]. Fractionation of the extract led to the isolation of the two bioflavonoids with the highest activity: amentoflavone, which was about 200 times more active (IC50 = 0.1 μg/mL) and less cytotoxic than SSHE (IC50 = 2.2 and 3 μg/mL, respectively on NIH/3T3 and J774.A1 cells), with a high selectivity index (SI) (22 and 30), robustaflavone, which was also active against L. amazonensis (IC50 = 2.8 µg/mL), but more cytotoxic, with IC50 = 25.5 µg/mL (SI = 9.1) on NIH/3T3 cells and IC50 = 3.1 µg/mL (SI = 1.1) on J774.A1 cells. The production of nitric oxide (NO) was lower in cells treated with amentoflavone (suggesting that NO does not contribute to the leishmanicidal mechanism in this case), while NO release was higher after treatment with robustaflavone. S. sellowii may be a potential source of biflavonoids that could provide promising compounds for the treatment of cutaneous leishmaniasis.

Show MeSH
Related in: MedlinePlus