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The TcI and TcII Trypanosoma cruzi experimental infections induce distinct immune responses and cardiac fibrosis in dogs.

Duz AL, Vieira PM, Roatt BM, Aguiar-Soares RD, Cardoso JM, Oliveira FC, Reis LE, Tafuri WL, Veloso VM, Reis AB, Carneiro CM - Mem. Inst. Oswaldo Cruz (2014)

Bottom Line: In addition, CD8+ T-lymphocytes isolated from the peripheral blood produced higher levels of interleukin (IL)-4.In the chronic phase, a significant increase in the number of inflammatory cells was detected in the right atrium.In the chronic phase, significant heart inflammation and fibrosis were observed, demonstrating that strains of different DTUs interact differently with the host.

View Article: PubMed Central - PubMed

Affiliation: Laboratório de Imunopatologia, Núcleo de Pesquisa em Ciências Biológicas, Universidade Federal de Ouro Preto, Ouro Preto, MG, Brasil.

ABSTRACT
Trypanosoma cruzi infection may be caused by different strains with distinct discrete typing units (DTUs) that can result in variable clinical forms of chronic Chagas disease. The present study evaluates the immune response and cardiac lesions in dogs experimentally infected with different T. cruzi strains with distinct DTUs, namely, the Colombian (Col) and Y strains of TcI and TcII DTU, respectively. During infection with the Col strain, increased levels of alanine aminotransferase, erythrocytes, haematocrit and haemoglobin were observed. In addition, CD8+ T-lymphocytes isolated from the peripheral blood produced higher levels of interleukin (IL)-4. The latter suggests that during the acute phase, infection with the Col strain may remain unnoticed by circulating mononuclear cells. In the chronic phase, a significant increase in the number of inflammatory cells was detected in the right atrium. Conversely, infection with the Y strain led to leucopoenia, thrombopoenia, inversion of the ratio of CD4+/CD8+ T-lymphocytes and alterations in monocyte number. The Y strain stimulated the production of interferon-γ by CD4+ and CD8+ T-lymphocytes and IL-4 by CD8+ T-cells. In the chronic phase, significant heart inflammation and fibrosis were observed, demonstrating that strains of different DTUs interact differently with the host.

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Related in: MedlinePlus

: morphometric analysis and photomicrographs of the heart showing thenumber of inflammation cells (haematoxylin and eosin) and fibrosis area (Massontrichrome) at 240 days after infection with Y strain (Y) or Colombian strain(Col) of Trypanosoma cruzi. The results are expressed as meannumber of cells or collagen area ± standard error. Significant differences at p< 0.05 are indicated by the symbols # for comparison between thenon-infected group (NI) and infected groups (Y or Col) and * for comparisondifferences between Y and Col groups at each time point. Bar = 50 μm.
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f04: : morphometric analysis and photomicrographs of the heart showing thenumber of inflammation cells (haematoxylin and eosin) and fibrosis area (Massontrichrome) at 240 days after infection with Y strain (Y) or Colombian strain(Col) of Trypanosoma cruzi. The results are expressed as meannumber of cells or collagen area ± standard error. Significant differences at p< 0.05 are indicated by the symbols # for comparison between thenon-infected group (NI) and infected groups (Y or Col) and * for comparisondifferences between Y and Col groups at each time point. Bar = 50 μm.

Mentions: Cardiac inflammation and fibrosis - Infection promoted inflammatorycell migration to the right atrium (Fig. 4).During the chronic phase, a focal inflammatory infiltrate was observed, which consistedpredominantly of mononuclear cells with lymphocyte morphology. The infection led tostatistically significant increases in the number of cells in groups infected witheither the Y or Col strain compared to the NI group. Moreover, the Y strain infectionfavoured collagen deposition and fibrosis formation was diffuse in the right atrium.This increase of areas occupied by fibrosis and the reduction of heart muscle werestatistically significant in the Y group compared to the NI and Col groups (Fig. 4).


The TcI and TcII Trypanosoma cruzi experimental infections induce distinct immune responses and cardiac fibrosis in dogs.

Duz AL, Vieira PM, Roatt BM, Aguiar-Soares RD, Cardoso JM, Oliveira FC, Reis LE, Tafuri WL, Veloso VM, Reis AB, Carneiro CM - Mem. Inst. Oswaldo Cruz (2014)

: morphometric analysis and photomicrographs of the heart showing thenumber of inflammation cells (haematoxylin and eosin) and fibrosis area (Massontrichrome) at 240 days after infection with Y strain (Y) or Colombian strain(Col) of Trypanosoma cruzi. The results are expressed as meannumber of cells or collagen area ± standard error. Significant differences at p< 0.05 are indicated by the symbols # for comparison between thenon-infected group (NI) and infected groups (Y or Col) and * for comparisondifferences between Y and Col groups at each time point. Bar = 50 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4325618&req=5

f04: : morphometric analysis and photomicrographs of the heart showing thenumber of inflammation cells (haematoxylin and eosin) and fibrosis area (Massontrichrome) at 240 days after infection with Y strain (Y) or Colombian strain(Col) of Trypanosoma cruzi. The results are expressed as meannumber of cells or collagen area ± standard error. Significant differences at p< 0.05 are indicated by the symbols # for comparison between thenon-infected group (NI) and infected groups (Y or Col) and * for comparisondifferences between Y and Col groups at each time point. Bar = 50 μm.
Mentions: Cardiac inflammation and fibrosis - Infection promoted inflammatorycell migration to the right atrium (Fig. 4).During the chronic phase, a focal inflammatory infiltrate was observed, which consistedpredominantly of mononuclear cells with lymphocyte morphology. The infection led tostatistically significant increases in the number of cells in groups infected witheither the Y or Col strain compared to the NI group. Moreover, the Y strain infectionfavoured collagen deposition and fibrosis formation was diffuse in the right atrium.This increase of areas occupied by fibrosis and the reduction of heart muscle werestatistically significant in the Y group compared to the NI and Col groups (Fig. 4).

Bottom Line: In addition, CD8+ T-lymphocytes isolated from the peripheral blood produced higher levels of interleukin (IL)-4.In the chronic phase, a significant increase in the number of inflammatory cells was detected in the right atrium.In the chronic phase, significant heart inflammation and fibrosis were observed, demonstrating that strains of different DTUs interact differently with the host.

View Article: PubMed Central - PubMed

Affiliation: Laboratório de Imunopatologia, Núcleo de Pesquisa em Ciências Biológicas, Universidade Federal de Ouro Preto, Ouro Preto, MG, Brasil.

ABSTRACT
Trypanosoma cruzi infection may be caused by different strains with distinct discrete typing units (DTUs) that can result in variable clinical forms of chronic Chagas disease. The present study evaluates the immune response and cardiac lesions in dogs experimentally infected with different T. cruzi strains with distinct DTUs, namely, the Colombian (Col) and Y strains of TcI and TcII DTU, respectively. During infection with the Col strain, increased levels of alanine aminotransferase, erythrocytes, haematocrit and haemoglobin were observed. In addition, CD8+ T-lymphocytes isolated from the peripheral blood produced higher levels of interleukin (IL)-4. The latter suggests that during the acute phase, infection with the Col strain may remain unnoticed by circulating mononuclear cells. In the chronic phase, a significant increase in the number of inflammatory cells was detected in the right atrium. Conversely, infection with the Y strain led to leucopoenia, thrombopoenia, inversion of the ratio of CD4+/CD8+ T-lymphocytes and alterations in monocyte number. The Y strain stimulated the production of interferon-γ by CD4+ and CD8+ T-lymphocytes and IL-4 by CD8+ T-cells. In the chronic phase, significant heart inflammation and fibrosis were observed, demonstrating that strains of different DTUs interact differently with the host.

Show MeSH
Related in: MedlinePlus