Limits...
The TcI and TcII Trypanosoma cruzi experimental infections induce distinct immune responses and cardiac fibrosis in dogs.

Duz AL, Vieira PM, Roatt BM, Aguiar-Soares RD, Cardoso JM, Oliveira FC, Reis LE, Tafuri WL, Veloso VM, Reis AB, Carneiro CM - Mem. Inst. Oswaldo Cruz (2014)

Bottom Line: In addition, CD8+ T-lymphocytes isolated from the peripheral blood produced higher levels of interleukin (IL)-4.In the chronic phase, a significant increase in the number of inflammatory cells was detected in the right atrium.In the chronic phase, significant heart inflammation and fibrosis were observed, demonstrating that strains of different DTUs interact differently with the host.

View Article: PubMed Central - PubMed

Affiliation: Laboratório de Imunopatologia, Núcleo de Pesquisa em Ciências Biológicas, Universidade Federal de Ouro Preto, Ouro Preto, MG, Brasil.

ABSTRACT
Trypanosoma cruzi infection may be caused by different strains with distinct discrete typing units (DTUs) that can result in variable clinical forms of chronic Chagas disease. The present study evaluates the immune response and cardiac lesions in dogs experimentally infected with different T. cruzi strains with distinct DTUs, namely, the Colombian (Col) and Y strains of TcI and TcII DTU, respectively. During infection with the Col strain, increased levels of alanine aminotransferase, erythrocytes, haematocrit and haemoglobin were observed. In addition, CD8+ T-lymphocytes isolated from the peripheral blood produced higher levels of interleukin (IL)-4. The latter suggests that during the acute phase, infection with the Col strain may remain unnoticed by circulating mononuclear cells. In the chronic phase, a significant increase in the number of inflammatory cells was detected in the right atrium. Conversely, infection with the Y strain led to leucopoenia, thrombopoenia, inversion of the ratio of CD4+/CD8+ T-lymphocytes and alterations in monocyte number. The Y strain stimulated the production of interferon-γ by CD4+ and CD8+ T-lymphocytes and IL-4 by CD8+ T-cells. In the chronic phase, significant heart inflammation and fibrosis were observed, demonstrating that strains of different DTUs interact differently with the host.

Show MeSH

Related in: MedlinePlus

: alanine aminotransferase (ALT) serum level before infection (0) and nine,30, 60, 90 and 240 days after infection with Y strain or Colombian strain (Col)of Trypanosoma cruzi. The results are expressed as mean enzymelevel ± standard error. Significant differences at p < 0.05 are indicated byletter a for comparisons with day 0. Dotted line indicates mean ± 2 standarddeviations for all animals at day 0 as the standard parameter. #: differencesbetween the non-infected group (NI) and infected groups (Y or Col).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4325618&req=5

f01: : alanine aminotransferase (ALT) serum level before infection (0) and nine,30, 60, 90 and 240 days after infection with Y strain or Colombian strain (Col)of Trypanosoma cruzi. The results are expressed as mean enzymelevel ± standard error. Significant differences at p < 0.05 are indicated byletter a for comparisons with day 0. Dotted line indicates mean ± 2 standarddeviations for all animals at day 0 as the standard parameter. #: differencesbetween the non-infected group (NI) and infected groups (Y or Col).

Mentions: T. cruzi infection mainly alters hepatic enzymes - Our resultsdemonstrate alterations in hepatic enzymes compared to values in non-infected dogs. Thisstandard parameter was calculated as the mean value of all animals before infection withand without the value of two-times the standard deviation (SD) (mean ± 2 SD). Theincreased value of ALT in the Y and Col groups in relation to standard parameters wassignificant at nine and 30 days after infection (d.a.i.) for the Y group compared withday 0 and the NI group. For the Col group, this increase was statistically significantat 9 d.a.i. compared with day 0 (Fig. 1).


The TcI and TcII Trypanosoma cruzi experimental infections induce distinct immune responses and cardiac fibrosis in dogs.

Duz AL, Vieira PM, Roatt BM, Aguiar-Soares RD, Cardoso JM, Oliveira FC, Reis LE, Tafuri WL, Veloso VM, Reis AB, Carneiro CM - Mem. Inst. Oswaldo Cruz (2014)

: alanine aminotransferase (ALT) serum level before infection (0) and nine,30, 60, 90 and 240 days after infection with Y strain or Colombian strain (Col)of Trypanosoma cruzi. The results are expressed as mean enzymelevel ± standard error. Significant differences at p < 0.05 are indicated byletter a for comparisons with day 0. Dotted line indicates mean ± 2 standarddeviations for all animals at day 0 as the standard parameter. #: differencesbetween the non-infected group (NI) and infected groups (Y or Col).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4325618&req=5

f01: : alanine aminotransferase (ALT) serum level before infection (0) and nine,30, 60, 90 and 240 days after infection with Y strain or Colombian strain (Col)of Trypanosoma cruzi. The results are expressed as mean enzymelevel ± standard error. Significant differences at p < 0.05 are indicated byletter a for comparisons with day 0. Dotted line indicates mean ± 2 standarddeviations for all animals at day 0 as the standard parameter. #: differencesbetween the non-infected group (NI) and infected groups (Y or Col).
Mentions: T. cruzi infection mainly alters hepatic enzymes - Our resultsdemonstrate alterations in hepatic enzymes compared to values in non-infected dogs. Thisstandard parameter was calculated as the mean value of all animals before infection withand without the value of two-times the standard deviation (SD) (mean ± 2 SD). Theincreased value of ALT in the Y and Col groups in relation to standard parameters wassignificant at nine and 30 days after infection (d.a.i.) for the Y group compared withday 0 and the NI group. For the Col group, this increase was statistically significantat 9 d.a.i. compared with day 0 (Fig. 1).

Bottom Line: In addition, CD8+ T-lymphocytes isolated from the peripheral blood produced higher levels of interleukin (IL)-4.In the chronic phase, a significant increase in the number of inflammatory cells was detected in the right atrium.In the chronic phase, significant heart inflammation and fibrosis were observed, demonstrating that strains of different DTUs interact differently with the host.

View Article: PubMed Central - PubMed

Affiliation: Laboratório de Imunopatologia, Núcleo de Pesquisa em Ciências Biológicas, Universidade Federal de Ouro Preto, Ouro Preto, MG, Brasil.

ABSTRACT
Trypanosoma cruzi infection may be caused by different strains with distinct discrete typing units (DTUs) that can result in variable clinical forms of chronic Chagas disease. The present study evaluates the immune response and cardiac lesions in dogs experimentally infected with different T. cruzi strains with distinct DTUs, namely, the Colombian (Col) and Y strains of TcI and TcII DTU, respectively. During infection with the Col strain, increased levels of alanine aminotransferase, erythrocytes, haematocrit and haemoglobin were observed. In addition, CD8+ T-lymphocytes isolated from the peripheral blood produced higher levels of interleukin (IL)-4. The latter suggests that during the acute phase, infection with the Col strain may remain unnoticed by circulating mononuclear cells. In the chronic phase, a significant increase in the number of inflammatory cells was detected in the right atrium. Conversely, infection with the Y strain led to leucopoenia, thrombopoenia, inversion of the ratio of CD4+/CD8+ T-lymphocytes and alterations in monocyte number. The Y strain stimulated the production of interferon-γ by CD4+ and CD8+ T-lymphocytes and IL-4 by CD8+ T-cells. In the chronic phase, significant heart inflammation and fibrosis were observed, demonstrating that strains of different DTUs interact differently with the host.

Show MeSH
Related in: MedlinePlus